Development of a feeding strategy for high cell and PHA density fed-batch fermentation of Ralstonia eutropha H16 from organic acids and their salts

Huschner, Franz; Grousseau, Estelle; Brigham, Christopher J.; Plassmeier, Jens; Popović, Milan; Rha, ChoKyun; Sinskey, Anthony J.

doi:10.1016/j.procbio.2014.12.004

Cited by 60 publications

(35 citation statements)

References 55 publications

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“…A look at more recent studies shows a shift toward addressing these issues. Firstly, several recent studies have achieved reasonably good results using more realistic waste carbon substrates, including agricultural residues [98,108], waste glycerol [99,183], VFAs [61,62,96,101], and plant oils [94,102]. Secondly, many of these studies are doing so without use of O 2 -enriched air, which can significantly improve productivity, but also adds significant expense, and is therefore not generally recommended [51,139].…”

Section: Discussionmentioning

confidence: 99%

“…Most often, feeding rates are predicted indirectly through using other parameters that can be rapidly and reliably measured using standard bioreactor equipment. Some of the more popular methods involve the use of off-gas CO 2 measurements [79,80,107,108], dissolved oxygen (DO) measurements [49,67,77,109], pH measurements [53,54], optical density [58,75], and combinations thereof [58,75,101]. In general, with these approaches, carbon limitation results in reduced oxygen demand, which manifests itself as a large drop in CO 2 production, reduced agitation rates, and also a rise in the DO signal.…”

Section: Production Of Phas In Fed-batch Bioreactor Systemsmentioning

confidence: 99%

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Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity

2018

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Microbial polyhydroxyalkanoates (PHAs) are promising biodegradable polymers that may alleviate some of the environmental burden of petroleum-derived polymers. The requirements for carbon substrates and energy for bioreactor operations are major factors contributing to the high production costs and environmental impact of PHAs. Improving the process productivity is an important aspect of cost reduction, which has been attempted using a variety of fed-batch, continuous, and semi-continuous bioreactor systems, with variable results. The purpose of this review is to summarize the bioreactor operations targeting high PHA productivity using pure cultures. The highest volumetric PHA productivity was reported more than 20 years ago for poly(3-hydroxybutryate) (PHB) production from sucrose (5.1 g L−1 h−1). In the time since, similar results have not been achieved on a scale of more than 100 L. More recently, a number fed-batch and semi-continuous (cyclic) bioreactor operation strategies have reported reasonably high productivities (1 g L−1 h−1 to 2 g L−1 h−1) under more realistic conditions for pilot or industrial-scale production, including the utilization of lower-cost waste carbon substrates and atmospheric air as the aeration medium, as well as cultivation under non-sterile conditions. Little development has occurred in the area of fully continuously fed bioreactor systems over the last eight years.

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Section: Discussionmentioning

confidence: 99%

Section: Production Of Phas In Fed-batch Bioreactor Systemsmentioning

confidence: 99%

Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity

2018

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“…The same strategy was tested by Kim et al [12] to feed fructose and/or γ-butyrolactone to C. necator ATCC 17699. A combination of pH-stat with DO-stat strategies was also proposed by Huschner et al [30] for feeding a mixture of sodium salts of acetic, propionic, and butyric acids to C. necator H16. There are no reports on the use of the DO-stat strategy to feed UCO or other oils containing substrates as the sole carbon sources for the fed-batch cultivation of C. necator.…”

Section: International Journal Of Polymer Sciencementioning

confidence: 99%

A Process Engineering Approach to Improve Production of P(3HB) byCupriavidus necatorfrom Used Cooking Oil

Cruz

Gouveia

Dionı́sio

et al. 2019

International Journal of Polymer Science

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Different feeding strategies, namely, exponential feeding and DO-stat mode, were implemented for the production of poly(3-hydroxybutyrate), P(3HB), by Cupriavidus necator DSM 428 with used cooking oil (UCO) as the sole carbon source. With the exponential feeding strategy, a cell dry mass of 21.3 ± 0.9 g L −1 was obtained, with a polymer content of 84.0 ± 4.5 wt.%, giving an overall volumetric productivity of 4.5 ± 0.2 g L −1 day −1 . However, the highest P(3HB) volumetric productivity, 12.6 ± 0.8 g L −1 day −1 , was obtained when the DO-stat mode was implemented together with the use of ammonium hydroxide for pH control, which served as an additional nitrogen source and allowed to reach higher cell dry mass (7.8 ± 0.6 g L −1 ). The P(3HB) obtained in all experiments had a high molecular mass, ranging from 0.6 × 10 5 to 2.6 × 10 5 g mol −1 , with low polydispersity indexes of 1.2-1.6. Melting and glass transition temperatures were also similar for the polymer produced with both cultivation strategy, 174°C and 3.0-4.0°C, respectively. The polymer exhibited a crystallinity ranging from 52 to 65%. The DO-stat strategy to feed oil containing substrates as the sole carbon sources was reported for the first time in this study, and the preliminary results obtained show that it is a promising strategy to improve P(3HB) production. Nevertheless, the process requires further optimization in order to make it economically viable.

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“…Presently the biodegradable polymer available in the largest quantity on the market is poly(lactic acid), but it is produced by traditional chemical synthesis [4][5][6][7][8][9][10]. However, in the past few decades, several fermentation techniques were developed in order to facilitate the production of microbial polyesters [11][12][13][14][15][16][17] and reduce the price of the product. One of the most important and most frequently studied microbial polyesters is poly-[(R)-3-hydroxybutyrate].…”

Section: Introductionmentioning

confidence: 99%

Enzymatic degradation of poly-[(R)-3-hydroxybutyrate]: Mechanism, kinetics, consequences

Polyák

Dohovits

Nagy

et al. 2018

International Journal of Biological Macromolecules

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Poly-[(R)-3-hydroxybutyrate] (PHB) films prepared by compression molding and solvent casting, respectively, were degraded with the intracellular depolymerase enzyme natively synthetized by the strain Bacillus megaterium. Quantitative analysis proved that practically only (R)-3-hydroxybutyric acid (3-HBA) forms in the enzyme catalyzed reaction, the amount of other metabolites or side products is negligible. The purity of the product was verified by several methods (UV-VIS spectroscopy, liquid chromatography, mass spectroscopy). Degradation was followed as a function of time to determine the rate of enzymatic degradation. Based on the Michaelis-Menten equation a completely new kinetic model has been derived which takes into consideration the heterogeneous nature of the enzymatic reaction. Degradation proceeds in two steps, the adsorption of the enzyme onto the surface of the PHB film and the subsequent degradation reaction. The rate of both steps depend on the preparation method of the samples, degradation proceed almost twice as fast in compression molded films than in solvent cast samples. The model can describe and predict the formation of the reaction product as a function of time. The approach can be used even for the commercial production of 3-HBA, the chemical synthesis of which is complicated and expensive.

show abstract

Development of a feeding strategy for high cell and PHA density fed-batch fermentation of Ralstonia eutropha H16 from organic acids and their salts

Cited by 60 publications

References 55 publications

Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity

Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity

A Process Engineering Approach to Improve Production of P(3HB) byCupriavidus necatorfrom Used Cooking Oil

Enzymatic degradation of poly-[(R)-3-hydroxybutyrate]: Mechanism, kinetics, consequences

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