Development of a genetic framework to improve the efficiency of bioactive delivery from blueberry

Abstract

In the present study, we applied a novel high-throughput in vitro gastrointestinal digestion model to phenotype bioaccessibility of phenolics in a diverse germplasm collection representing cultivated highbush blueberries. Results revealed significant (P < 0.05) differences between accessions, years, and accession by year interaction for relative and absolute bioaccessibility of flavonoids and phenolic acids. Broad sense heritability estimates revealed low to moderate inheritances of relative and absolute bioaccessibility, suggesting that besides environmental variables, genetics factors could control bioaccessibility of phenolics. Acylated anthocyanins had significantly higher relative bioaccessibility than non-acylated anthocyanins. Correlation analysis indicated that relative bioaccessibility did not show significant association with fruit quality or raw concentration of metabolites. The study also identified accessions that have high relative and absolute bioaccessibility values. Overall, combining the bioaccessibility of phenolics with genetic and genomic approaches will enable the identification of genotypes and genetic factors influencing these traits in blueberry.

Boosting the Bioaccessibility of Dietary Bioactives by Delivery as Protein–Polyphenol Aggregate Particles

“…Also of note, in a recent study analyzing bioaccessibility of blueberry polyphenols in a multi-stage in vitro digestion system, polyphenol bioaccessibility was highly dependent upon the genetic makeup of each blueberry genotype. 61 The same was recently observed for carotenoid bioaccessibility in spinach 62 and cassava. 63 These results indicate that the levels of bioactive phytochemicals inherently present in fruits and vegetables may not consistently predict levels of uptake after consumption of these same fruits and vegetables.…”

“…Also of note, in a recent study analyzing bioaccessibility of blueberry polyphenols in a multi-stage in vitro digestion system, polyphenol bioaccessibility was highly dependent upon the genetic makeup of each blueberry genotype . The same was recently observed for carotenoid bioaccessibility in spinach and cassava .…”

Autopolyploid inheritance and a heterozygous reciprocal translocation shape chromosome genetic behavior in tetraploid blueberry (Vaccinium corymbosum)

“…Blueberries (Vaccinium spp.) are well-recognized as a rich source of health-promoting phytochemicals, which have contributed to a rapid increase in consumer demand and production over the past two decades (Mengist et al, 2020a). Domesticated only c. 100 yr ago, tetraploid highbush (V. corymbosum; HB) is the most widely grown blueberry, accounting for vast majority of the US blueberry production (Mengist et al, 2020b).…”

Mixed Berry Juice and Cellulose Fiber Have Differential Effects on Peripheral Blood Mononuclear Cell Respiration in Overweight Adults

“…Anthocyanin species were designated as 3-O-glucoside by the comparison of chromatography and SIR to authentic standards. The designation of arabinoside and galactoside was performed based on elution order from our previous separations [ 20 , 22 ].…”