Development of a Genetic Transformation Method for Seabuckthorn (&amp;lt;i&amp;gt;Hippophae rhamnoides&amp;lt;/i&amp;gt; L.)

Sriskandarajah, Sridevy; Clapham, David; Lundquist, Per-Olof

doi:10.4236/ajps.2014.55067

Cited by 5 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gene introduction and stress-tolerant studies on seabuckthorn were initially performed with conventional methods that led to successful transformation. Stable trans formants of H. rhamnoides with insertion of lectin gene [53] and kanamycin resistance [54] are brought out by the standard transformation techniques (Table 4).…”

Section: Preliminary Genetic Modificationsmentioning

confidence: 99%

A review on genetic diversity, micropropagation and transformations in the high-value medicinal plant of Himalayas-Seabuckthorn (Hippophae sp.)

Balasubramaniam,

Ganeshan,

Natesan

et al. 2024

J Pharmacogn Phytochem

View full text Add to dashboard Cite

Seabuckthorn (Hippophae sp.) is popularly known as the "Gold Rush" for its commercial value. It holds well-established vegetative propagation and in vitro regeneration techniques. Accordingly, micropropagation of Hippophae sp. from multiple explants follows the developmental stages of establishment, shoot proliferation, and rooting. The successful in vitro propagation protocols utilized Murashige and Skoog medium and Woody Plant Medium supplemented with cytokinin for shoot organogenesis, somatic embryogenesis, and auxins for high rooting frequency. Subsequent acclimatization of Hippophae sp. with a reasonable survival rate was recorded under greenhouse conditions. The genetic transformations in seabuckthorn are yet to be studied in detail. Still, initial studies were performed with conventional methods using Agrobacterium rhizogenes (indirect) and biolistics (direct), which could support extensive transformation research in the future for commercialization and conservation. This short review provides an overview of compiled works on the tissue culture of Hippophae rhamnoides and Hippophae salicifolia that could assist in further assessment and vast applications.

show abstract

Section: Preliminary Genetic Modificationsmentioning

confidence: 99%

A review on genetic diversity, micropropagation and transformations in the high-value medicinal plant of Himalayas-Seabuckthorn (Hippophae sp.)

Balasubramaniam,

Ganeshan,

Natesan

et al. 2024

J Pharmacogn Phytochem

View full text Add to dashboard Cite

show abstract

“…Still, the notion of using gene transfer or gene editing methods may prove unacceptable for a crop destined for the health food market. Since only a few protocols are available for regeneration [83] and transformation of sea buckthorn [84], a lack of efficient regeneration protocols could also hamper the application of these methods.…”

Section: Sea Buckthorn Breeding Around the Worldmentioning

confidence: 99%

The Systematics, Reproductive Biology, Biochemistry, and Breeding of Sea Buckthorn—A Review

Nybom,

Ruan,

Rumpunen

2023

Genes

View full text Add to dashboard Cite

Both the fruit flesh and seeds of sea buckthorn have multiple uses for medicinal and culinary purposes, including the valuable market for supplementary health foods. Bioactive compounds, such as essential amino acids, vitamins B, C, and E, carotenoids, polyphenols, ursolic acid, unsaturated fatty acids, and other active substances, are now being analyzed in detail for their medicinal properties. Domestication with commercial orchards and processing plants is undertaken in many countries, but there is a large need for improved plant material with high yield, tolerance to environmental stress, diseases, and pests, suitability for efficient harvesting methods, and high contents of compounds that have medicinal and/or culinary values. Applied breeding is based mainly on directed crosses between different subspecies of Hippophae rhamnoides. DNA markers have been applied to analyses of systematics and population genetics as well as for the discrimination of cultivars, but very few DNA markers have as yet been developed for use in selection and breeding. Several key genes in important metabolic pathways have, however, been identified, and four genomes have recently been sequenced.

show abstract

“…Particle bombardment method for transforming sea buckthorn was tested by Sriskandarajah et al, 2014. The early stages of induced adventitious shoots from roots were chosen as a novel target tissue for the transformation procedure.…”

Section: Achievements In Biotechnologymentioning

confidence: 99%

Sea Buckthorn a Boon for Trans-Himalayan Region of Ladakh: A Review

Lamo¹,

Solanki²

2019

View full text Add to dashboard Cite

Sea buckthorn (Hippophae rhamnoides L.) is an ecologically and economically important plant species and is currently being domesticated in various parts of the world including India. Sea buckthorn achieved a unique status in the trans-Himalayan Ladakh because of its immense medicinal and therapeutic potentials. High nutrient and medicinal values of the fruit attracts researchers to exploit in the field of biotechnology, neutraceutical, pharmaceutical, cosmetic and environmental sciences. However, unrestrained exploitation and even destruction of sea buckthorn resources leads to loss in its diversification and extinction. Therefore, there is need to conserve the wild genotypes of sea buckthorn to make them available as a source for breeding of new plant varieties in future and judicious utilization of wild species can bring more benefit to mankind throughout the world. Application of DNA markers to facilitate marker-aided selection (MAS) for diversity studies and identification of cold resistant genes have been developed, further improvements is still needed. Advances in plant genomics, genetic mapping and QTL analysis provide further means to improve the understanding of crop diversity at species and gene levels, it also accelerated the dissection of genetic control of important traits and development of high value genotypes in the context of breeding programs. Advances in biotechnology provided many tools that will benefit in providing quality planting material, generation of new cultivars and mass propagation therefore help in area expansion in areas like Ladakh. DRDO is an active organization in collaboration with other institutes to enhance the research field in sea buckthorn and grant patent on its products. There is no doubt that the future holds great promise for sea buckthorn cultivation in Ladakh and efforts need to be made to certify sea buckthorn as organic.

show abstract

Development of a Genetic Transformation Method for Seabuckthorn (<i>Hippophae rhamnoides</i> L.)

Cited by 5 publications

References 15 publications

A review on genetic diversity, micropropagation and transformations in the high-value medicinal plant of Himalayas-Seabuckthorn (Hippophae sp.)

A review on genetic diversity, micropropagation and transformations in the high-value medicinal plant of Himalayas-Seabuckthorn (Hippophae sp.)

The Systematics, Reproductive Biology, Biochemistry, and Breeding of Sea Buckthorn—A Review

Sea Buckthorn a Boon for Trans-Himalayan Region of Ladakh: A Review

Contact Info

Product

Resources

About