Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers

Yang, Zaijun; Chen, Zhenyong; Peng, Zhengsong; Yu, Yan; Liao, Mingli; Wei, Shuhong

doi:10.1186/s12864-017-3960-7

Cited by 45 publications

(34 citation statements)

References 27 publications

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“…It was systemically observed and studied from 2012, and our work showed that the multi-pistil trait can be stably transmitted to offspring. Our study showed that the multi-pistil trait is controlled by the single semi-dominant gene 12TP, differing from previous reports (Chen et al 1983;Peng 2003;Peng et al 2013;Yang et al 2017).…”

Section: Multi-pistil Gene 12tp Is a Semi-dominant Genecontrasting

confidence: 99%

“…Differences in cytoplasms (Wu et al 2000) or the presence of foreign cytoplasms (e.g., that of Aegilops kotschyi or Aegilops ventricosa), have been additionally shown to inhibit the expression of some 'tri-grain' genes (Ma et al 2000). The multi-pistil genes are located on 2DL, 5DS, 6BS and 6B (Tong and Tong 1984;Shen et al 1992;Ma et al 2000;Peng et al 2008;Wang et al 2009;Yang et al 2017). Map-based cloning, which requires finding closely linked markers, is the most effective method for the cloning of multipistil genes.…”

Section: Introductionmentioning

confidence: 99%

“…reports have described three pistil (TP) mutants, in which spike morphology is normal but two additional pistils initiate after emergence of the first one, resulting in three pistils developing per floret (Chen et al 1983;Tong and Tong 1984;Peng 2003;Wang et al 2009). As a result, three seeds can be cultivated per floret, increasing the number of grains per spike (Yang et al 2017). Most wheat multi-pistil mutants belong to 'tri-grain' wheat morphology, in which florets contain a lemma, a palea, two lodicules, three stamens, and three fertile pistils.…”

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confidence: 99%

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Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP

Zhu

et al. 2019

Journal of Integrative Agriculture

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We identified a wheat (Triticum aestivum L.) multi-pistil mutant from an F 2 breeding population in 2012, named 12TP (three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait. Using homozygous normal and multi-pistil lines (near isogenic lines; NILs) derived from the original mutant 12TP, a simple sequence repeat (SSR) marker assay located the 12TP locus on chromosome arm 2DL. Four SSR markers were linked to 12TP and their order was Xcfd233-Xcfd62-12TP-Xwmc41-Xcfd168 at 15.85, 10.47, 2.89, and 10.37 cM. The average genetic expressivity of the trait 'three pistils in one floret' was more than 98% in seven homozygous 12TP lines; however, the average genetic expressivity in heterozygous F 1 plants was about 49%. Thus, the 12TP is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants. Mutant 12TP is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat.

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Section: Multi-pistil Gene 12tp Is a Semi-dominant Genecontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP

Zhu

et al. 2019

Journal of Integrative Agriculture

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“…The presence of transcription factors (TF) binding motifs were analyzed with the PlantTFcat online tool (http://plantgrn.noble.org/PlantTFcat) [15]. SSRs (Simple Sequence Repeats), a type of molecular marker, is a potent tool for genetic studies and marker assisted selection (MAS) in many crops [16, 17]. In addition, in this research, we adopted the MISA search tool for SSR screening [18].…”

Section: Methodsmentioning

confidence: 99%

Transcriptome analysis reveals salinity responses in four Tartary buckwheat cultivars

Song

Liu

Wang

et al. 2019

Preprint

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12Tartary buckwheat (Fagopyrum tataricum) is an annual herbaceous crop with a higher content of rutin and 13 flavonoids than other crops. In order to understand the salt tolerance mechanism of Tartary buckwheat, we 14 conducted a transcriptome sequencing study. Two existing and two new salt-tolerant varieties of Tartary 15 buckwheat were used as experimental materials. These varieties were subjected to 100 mM NaCl for 48 16 hours treatment at the 3 leaf stage. Leaf RNA was extracted from 8 samples and performed RNA-seq. We de 17 novo assembled reads into a transcriptome dataset containing 62,637 unigenes with N50 length of 1596 bp. 18A total of 205. 2 million clean reads were obtained, and 33,495 (53.47%) of them were annotated. GO and 19 KEGG classification showed enrichment of these unigenes in 47 sub-categories, 25 KOG functional 20 categories and 129 pathways, respectively. With 11 contrasts, we obtained many differentially expressed 21 genes (DEGs) of transcriptome data in 8 samples. The relative expression of Unigene0009545 and 22Unigene0057231 in M1 and that of Unigene0023096 and Unigene0060321 in M2 was increased significantly 23 under salt stress and may be involved in salt tolerance. These findings will provide valuable reference 24 genetic information for the study on salt tolerance mechanism in plants and assist in cultivating strong 25 salt-tolerant varieties of Tartary buckwheat for greater yield in saline-alkali soil. 26

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“…GBS is a cost-efficient and effective genotyping method (Yang et al, 2017;Bhattarai et al, 2018;Hackett et al, 2018). In this study, 390.30 Gb clean reads were generated.…”

Section: Genetic Purity Genetic Distance and Relationshipsmentioning

confidence: 99%

Peer Review #2 of "Development of core-collections for Guizhou tea genetic resources and GWAS of leaf size using SNP developed by genotyping-by-sequencing (v0.1)"

2020

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An accurate depiction of the genetic relationship, the development of core collection, and genome-wide association analysis (GWAS) are key for the effective exploitation and utilization of genetic resources. Here, genotyping-by-sequencing (GBS) was used to characterize 415 tea accessions mostly collected from the Guizhou region in China. A total of 30,282 high-quality SNPs was used to estimate the genetic relationships, develop core collections, and perform GWAS. We suggest 198 and 148 accessions to represent the core set and mini-core set, which consist of 47% and 37% of the whole collection, respectively, and contain 93-95% of the total SNPs. Furthermore, the frequencies of all alleles and genotypes in the whole set were very well retained in the core set and mini-core set. The 415 accessions were clustered into 14 groups and the core and the mini-core collections contain accessions from each group, species, cultivation status and growth habit. By analyzing the significant SNP markers associated with multiple traits, nine SNPs were found to be significantly associated with four leaf size traits, namely MLL, MLW, MLA and MLSI (P<1.655E-06).This study characterized the genetic distance and relationship of tea collections, suggested the core collections, and established an efficient GWAS analysis of GBS result.

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Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers

Cited by 45 publications

References 27 publications

Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP

Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP

Transcriptome analysis reveals salinity responses in four Tartary buckwheat cultivars

Peer Review #2 of "Development of core-collections for Guizhou tea genetic resources and GWAS of leaf size using SNP developed by genotyping-by-sequencing (v0.1)"

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