2019
DOI: 10.1111/jphp.13152
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Development of a high-performance liquid chromatography with tandem mass spectrometry method for identifying common adulterant content in saffron (Crocus sativus L.)

Abstract: Objectives This study aimed to develop an efficient and reliable method for estimating common adulterants in saffron by detecting their characteristic components to warrant its efficacy and regular use as a highly valuable medicinal herb. Methods A selective and sensitive high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) method was developed to estimate the common adulterants in saffron from corn stigma, chrysanthemum and safflower through the simultaneous determination of speci… Show more

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Cited by 15 publications
(6 citation statements)
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“…In 2019, high performance liquid chromatography (HPLC) with tandem mass spectroscopy was used to detect corn stigma, chrysanthemum, and safflower as adulterants in saffron, through the identification of the components of allantoin, chlorogenic acid (ChA) and hydroxysafflor yellow A (HSYA) (Kong et al, 2019). Using this technique, along with gel permeation chromatography (GPC), Sudan dyes were detected in Egyptian spices with a limit of quantification of 0.1 mg/kg (Sebaei et al, 2019).…”
Section: Chromatographymentioning
confidence: 99%
“…In 2019, high performance liquid chromatography (HPLC) with tandem mass spectroscopy was used to detect corn stigma, chrysanthemum, and safflower as adulterants in saffron, through the identification of the components of allantoin, chlorogenic acid (ChA) and hydroxysafflor yellow A (HSYA) (Kong et al, 2019). Using this technique, along with gel permeation chromatography (GPC), Sudan dyes were detected in Egyptian spices with a limit of quantification of 0.1 mg/kg (Sebaei et al, 2019).…”
Section: Chromatographymentioning
confidence: 99%
“…Many scholars have used the LC‐MS approach to discover markers of adulteration of saffron. The allantoin, chlorogenic acid, and hydroxysafflor yellow A content is helpful for the detection of saffron samples adulterated with corn stigma, chrysanthemum, and safflower (Kong et al, 2019). Kaempferol derivatives are authenticity markers and geniposide is an adulteration marker for saffron (Guijarro‐Díez et al, 2017).…”
Section: Mass Spectrometry‐based Metabolomics For Determination Of Ad...mentioning
confidence: 99%
“…Specifically, it may be undeclared additions of colored stamens [ 4 , 7 10 ], styles [ 10 12 ], or petals [ 9 , 10 ] of the saffron itself. For this purpose, petals of safflower ( Carthamus tinctorius L.) [ 1 , 2 , 4 , 6 8 , 10 23 ], petals of calendula ( Calendula officinalis L.) [ 1 , 2 , 4 , 7 , 10 13 , 15 , 17 , 20 ], rhizomes of turmeric ( Curcuma longa L.) [ 1 , 2 , 4 , 7 , 8 , 10 , 12 , 15 , 17 , 18 , 20 , 21 , 23 ], extracts of gardenia fruits ( Gardenia jasminoides J. Ellis) [ 1 , 4 , 5 , 7 , 8 , 17 , 24 27 ], petals of mountain arnica ( Arnica montana L.) [ 1 , 2 , 15 , 17 ], dye from seeds of achiote ( Bixa orellana L.) [ 2 , 15 , 17 ], petals of chrysanthemum (C hrysanthemum morifolium L.) [ 6 , 16 , 17 ], petals of hemerocallis ( Hemerocallis sp.) [ 2 , 15 , 17 ], stigmas of crocus vernus ( Crocus vernus (L.) Hill) [ 2 , 15 ], dye from pomegranate fruits or petals ( Punica granatum L.) [ 10 , 17 ], dye from flowers of buddleia ( Buddleja officinalis ...…”
Section: Introductionmentioning
confidence: 99%