Development of a high-throughput and sensitive assay of fusion genes in lung cancer by array-based MALDI-TOFMS

Abstract

This study established a high-throughput, sensitive and cost-effective method to detect three lung fusion genes of 96 samples at one time.

MoS2-Assisted LDI Mass Spectrometry for the Detection of Small Molecules and Quantitative Analysis of Sulfonamides in Serum

“…Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), with the merits of simplicity, high speed, high throughput, high salt tolerance, and low sample consumption, is superior to numerous mass spectrometry analytical techniques. 11−13 It has become an excellent and powerful tool not only for the analysis of macromolecules such as proteins, 14,15 peptides, 16 nucleic acids, 17,18 and polymers 19 but also for determination of small molecules with the aid of nanomaterials as matrices such as metal/metal oxides, carbon-based materials, and silicon-based nanostructures. 20,21 However, there are still several shortcomings that need to be addressed.…”

“…However, high organic reagent consumption, time-consuming sample pretreatment, and complicated operation procedures are the primary obstacles, especially in analyzing high-throughput samples, if needed. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), with the merits of simplicity, high speed, high throughput, high salt tolerance, and low sample consumption, is superior to numerous mass spectrometry analytical techniques. − It has become an excellent and powerful tool not only for the analysis of macromolecules such as proteins, , peptides, nucleic acids, , and polymers but also for determination of small molecules with the aid of nanomaterials as matrices such as metal/metal oxides, carbon-based materials, and silicon-based nanostructures. , …”