Development of a high throughput (HT) Raman spectroscopy method for rapid screening of liquid blood plasma from prostate cancer patients

Medipally, Dinesh; Maguire, Adrian; Bryant, Jane; Armstrong, John G.; Dunne, Mary; Finn, Marie; Lyng, Fiona M.; Meade, Aidan D.

doi:10.1039/c6an02100j

Cited by 57 publications

(40 citation statements)

References 32 publications

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“…This set-up also has the added advantage of providing high quality, consistent Raman spectra from a sample volumes as low as 1μL. Medipally et al also reported the benefits of using inverted Raman geometry to analyse plasma samples of small volume (20µL) from prostate cancer patients [50]. Note, enhanced methods such as Surface Enhanced Raman Spectroscopy (SERS) are not considered in this review.…”

Section: Real Time Analysismentioning

confidence: 99%

“…In the study conducted by Medippally et. al., rubber band baseline correction, Savitzky-Golay smoothing algorithm and vector-normalisation was performed on the raw Raman spectra of liquid plasma prior to post processing, in R based statistical software [50]. In studies aimed at early diagnosis of oral cancer using Raman spectroscopy by Sahu et.…”

Section: Data Preprocessingmentioning

confidence: 99%

“…The adapted EMSC algorithm can also be employed to remove known spectral interferents. In several studies of human serum, β-carotene has been observed as a string contributor to the spectrum, particularly when using 532nm or lower as source, as the scattering from the conjugated antioxidant species are resonantly enhanced [29,50,83]. The LMWF species are not easily removed by centrifugal separation [69], but their contributions can be effectively "digitally" removed using the adapted EMSC protocol.…”

Section: S~ [ × ] + [ × ] + [∑ ]mentioning

confidence: 99%

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Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

2020

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There is compelling evidence in the literature to support the application of Raman spectroscopy for analysis of bodily fluids in their native liquid state. Naturally, the strategies described in the literature for Raman spectroscopic analysis of liquid samples have advantages and disadvantages. Herein, recent advances in the analysis of plasma/serum in the liquid state are reviewed. The potential advantages of Raman analysis in the liquid form over the commonly employed infrared absorption analysis in the dried droplet form are initially highlighted.Improvements in measurement protocols based on inverted microscopic geometries, clinically adaptable substrates, data preprocessing and analysis, and applications for routine monitoring of patient health as well as therapeutic administration are reviewed. These advances suggest that clinical translation of Raman spectroscopy for rapid biochemical analysis can be a reality.In the future, this method will prove to be highly beneficial to clinicians for rapid screening and monitoring of analytes and drugs in the biological fluids, and to the patients themselves, enabling early treatment, before the disease becomes symptomatic, allowing early recovery.

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Section: Real Time Analysismentioning

confidence: 99%

Section: Data Preprocessingmentioning

confidence: 99%

Section: S~ [ × ] + [ × ] + [∑ ]mentioning

confidence: 99%

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Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

2020

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“…В настоящее время в литературе нет единого мнения о принадлежности этих сигналов каким-либо конкретным химическим группам. Большинство исследователей склоняется к тому, что основной вклад в интенсивность этих пиков вносит β-каротин [20,21] ряд авторов ассоциируют данные полосы поглощения с сигналами от фенилаланина, D-маннозы, α-спирали и коллагена [22,23]. Различная интенсивность пиков спектров комбинационного рассеяния света в определенной степени отражает существующие различия в интегральных метаболомических профилях сывороток крови пациентов с КРР [24].…”

Section: результаты и обсуждениеunclassified

Использование Спектральной Эллипсометрии И Спектроскопии Комбинационного Рассеяния Света В Скрининговой Диагностике Колоректального Рака

Кручинин

Кручинина

Прудникова

et al. 2019

Журнал Технической Физики

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Diagnostic monitoring of groups of healthy patients and patients with colorectal cancer (CRC) at various stages of the development of the disease was carried out using Raman spectroscopy (CRS) and measuring the state of polarized light in the vicinity of surface plasmon resonance (SPR) observation conditions. For the diagnosis, the reaction of serum antigens with antibodies to serum M2-pyruvate kinase (M2-PK) was used near the observation conditions of SPR, for which we used the Ellips-SPEC spectral ellipsometric complex created in the Institute of Semiconductor Physics of the Siberian Branch of the Russian Academy of Sciences (ISP SB RAS). The complex has high sensitivity, accuracy and non-destructive impact on the sample under study. The intensities of the peaks at 1005, 1157 and 1520 cm-1 in the CRS spectra of CRC patients as compared with healthy individuals as well as the intensity of the equilibrium plasmon signal were significantly lower, correlating with the stage of the disease and the presence of metastases. It allows us to consider these optical methods as a promising diagnostic approaches in the diagnosis of CRC, including in the early stages of the disease.

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“…For example, proof-of-principle studies have successfully demonstrated that RS of blood plasma/serum can discriminate between healthy controls and oral cancer [9], head and neck cancer [10], and prostate cancer [11] with sensitivity and specificity above 75%. RS has also been applied to investigate intact RBCs and hemoglobin to detect biomarkers related to malaria, diabetes, genetic blood disorders, and heart failure [5].…”

Section: Introductionmentioning

confidence: 99%

Development of a flow cell based Raman spectroscopy technique to overcome photodegradation in human blood

Hansson

Allen

Qutob

et al. 2019

Biomed. Opt. Express

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Raman spectroscopy of blood offers significant potential for label-free diagnostics of disease. However, current techniques are limited by the use of low laser power to avoid photodegradation of blood; this translates to a low signal to noise ratio in the Raman spectra. We developed a novel flow cell based Raman spectroscopy technique that provides reproducible Raman spectra with a high signal to noise ratio and low data acquisition time while ensuring a short dwell time in the laser spot to avoid photodamage in blood lysates. We show that our novel setup is capable of detecting minute changes in blood lysate spectral features from natural aging. Moreover, we demonstrate that by rigorously controlling the experimental conditions, the aging effect due to natural oxidation does not confound the Raman spectral measurements and that blood treated with hydrogen peroxide to induce oxidative stress can be discriminated from normal blood with a high accuracy of greater than 90% demonstrating potential for use in a clinical setting.

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Development of a high throughput (HT) Raman spectroscopy method for rapid screening of liquid blood plasma from prostate cancer patients

Abstract: High throughput Raman spectroscopy method for rapid and accurate diagnosis of prostate cancer using liquid plasma samples.

Cited by 57 publications

References 32 publications

Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

Использование Спектральной Эллипсометрии И Спектроскопии Комбинационного Рассеяния Света В Скрининговой Диагностике Колоректального Рака

Development of a flow cell based Raman spectroscopy technique to overcome photodegradation in human blood

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