Development of a highly sensitive time-resolved fluoroimmunoassay for the determination of trace salbutamol in environmental samples

Fang, Song; Zhang, Yizhi; Liu, Xue; Qiu, Jun; Liu, Zhenjiang; Kong, Fanyu

doi:10.1016/j.scitotenv.2019.05.057

Cited by 29 publications

(6 citation statements)

References 31 publications

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“…The specific binding of immunoreagents is the basis of TRFICA, as with other immunoassays. An appropriate antigen-antibody ratio can enhance the binding of specific sites and improve TRFICA performance [29]. Thus, the concentrations of mAb 3B4 and the coating antigen DNC-4-BSA were screened using checkerboard titration.…”

Section: Optimization Of the Concentrations Of Mab And Coating Antigenmentioning

confidence: 99%

A Europium Nanosphere-Based Time-Resolved Fluorescent Immunochromatographic Assay for the Rapid Screening of 4,4′-Dinitrocarbanilide: Aiming at Improving Strip Method Performance

et al. 2023

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Considering that the strip method is simple and convenient for users, a Europium nanosphere-based time-resolved fluorescent immunochromatographic assay (TRFICA) for the rapid screening of 4,4′-dinitrocarbanilide (DNC) was developed to improve the performance of strip assays. After optimization, TRFICA showed IC50, the limit of detection, and cut-off values of 0.4, 0.07, and 5.0 ng mL−1, respectively. No significant cross-reactivity (CR < 0.1%) with 15 DNC analogs was observed in the developed method. TRFICA was validated for DNC detection in spiked chicken homogenates, and recoveries ranged from 77.3% to 92.7%, with coefficients of variation of <14.9%. Moreover, the time needed for the detection procedure, including the sample pre-treatment, was less than 30 min for TRFICA, which had never been achieved before in other immunoassays. The newly developed strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique for DNC analysis in chicken muscle.

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Section: Optimization Of the Concentrations Of Mab And Coating Antigenmentioning

confidence: 99%

A Europium Nanosphere-Based Time-Resolved Fluorescent Immunochromatographic Assay for the Rapid Screening of 4,4′-Dinitrocarbanilide: Aiming at Improving Strip Method Performance

et al. 2023

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“…Indirect competitive ELISAs have been developed to detect β 2 -agonists, including phenylethanolamine A [40], ractopamine [41], zilpaterol [42], clenbuterol [43,44] and salbutamol [45,46] in various types of samples. In these assays, the β 2 -agonist coated on the microtiter plate competes with β 2 -agonist in samples for binding to the primary anti-β 2 -agonist antibody.…”

Section: Indirect Competitive Elisamentioning

confidence: 99%

“…Most of the above indirect competitive ELISAs used HRP to label the secondary antibody. Fang et al [46] used Eu 3+ -labeled secondary antibody in the ELISA to detect salbutamol. Han et al [41] developed a gold nanoparticle-based indirect competitive ELISA to detect ractopamine by using a secondary antibody labeled with catalase through biotin-streptavidin interaction.…”

Section: Indirect Competitive Elisamentioning

confidence: 99%

Current Advances in Immunoassays for the Detection of β2-Agonists

Ouyang

2022

Foods

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β2-agonists are a group of synthetic phenylethanolamine compounds which are traditionally used for treating bronchospasm. These compounds can also increase skeletal muscle mass and decrease body fat. The illegal use of β2-agonists in food-producing animals results in residue of β2-agonists in edible tissues and causes adverse health effects in humans. Thus, the detection of β2-agonists at trace level in complex sample matrices is of great importance for monitoring the abuse of β2-agonists. Many methods have been developed to detect β2-agonists. Among them, a variety of antigen–antibody interaction-based techniques have been established to detect β2-agonists in various samples, including animal feed, urine, serum, milk, tissues and hair. In this review, we summarized current achievement in the extraction of β2-agonists from testing samples and detection of β2-agonists using immunological techniques. Future perspectives were briefly discussed.

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“…Studies have shown that SAL has the possibility of entering the ecological environment through animal feces and urine. While causing environmental pollution, it then enters the human body through indirect channels (Fang et al, 2019). SAL has already been a widespread environmental pollutant (Depaolini et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Docking guided phase display to develop fusion protein with novel scFv and alkaline phosphatase for one-step ELISA salbutamol detection

Hu,

Yang,

Chen

et al. 2023

Front. Microbiol.

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IntroductionSalbutamol (SAL) is a β2 adrenergic receptor agonist which has potential hazardous effects for human health. It is very important to establish a sensitive and convenient method to monitor SAL.MethodsHere we introduce a method to combine the information from docking and site specific phage display, with the aim to obtain scFv with high affinity to SAL. First, single chain variable fragment (scFv) antibodies against SAL were generated through phage display. By using molecular docking approach, the complex structure of SAL with antibody was predicted and indicated that H3 and L3 contribute to the binding. Then new libraries were created by randomization specific residues located on H3 and L3 according to the docking results.Results and discussionAnti-SAL scFv antibodies with high efficiency were finally identified. In addition, the selected scFv was fused with alkaline phosphatase and expressed in E coli to develop a rapid and low-cost one step ELISA to detect SAL.

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Development of a highly sensitive time-resolved fluoroimmunoassay for the determination of trace salbutamol in environmental samples

Cited by 29 publications

References 31 publications

A Europium Nanosphere-Based Time-Resolved Fluorescent Immunochromatographic Assay for the Rapid Screening of 4,4′-Dinitrocarbanilide: Aiming at Improving Strip Method Performance

A Europium Nanosphere-Based Time-Resolved Fluorescent Immunochromatographic Assay for the Rapid Screening of 4,4′-Dinitrocarbanilide: Aiming at Improving Strip Method Performance

Current Advances in Immunoassays for the Detection of β2-Agonists

Docking guided phase display to develop fusion protein with novel scFv and alkaline phosphatase for one-step ELISA salbutamol detection

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