Development of a highly specific co-dominant marker for genotyping the Ph-3 (tomato late blight resistance) locus by comparing cultivated and wild ancestor species

Ren, Zhiyong; You, Z. P.; Munir, Sadia; Zhang, Yuyang; Li, Hanxia; Zhang, Junhong; Wang, Taotao; Zheng, Wei; Ye, Zhibiao

doi:10.1007/s11032-019-0953-z

Cited by 5 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As for the Ph-3 and Ty-3 genes, we found eight (E11, E36, E45, E48, E55, E109, E111 and PDLUC) and three (E55, PDLUC and PDVIT) genotypes evidencing the homozygous resistance alleles for Ph-3 and Ty-3, respectively. The first gene is usually incorporated in breeding programs [33], whereas the interest in the second one is crucial since the vector of the TYLCV, the white tobacco fly Bemisia tabaci, shows an increased proliferation under HT [7]. Three additional markers targeting the genes Sw-5, Tm-2 and Ve-1 were designed in the present work, deriving from the multiple alignment of resistant and susceptible alleles retrieved from GenBank accessions (Figures S2 and S3).…”

Section: Molecular Screening For Resistance Genes In Parental Linesmentioning

confidence: 99%

Accelerating the Development of Heat Tolerant Tomato Hybrids through a Multi-Traits Evaluation of Parental Lines Combining Phenotypic and Genotypic Analysis

Olivieri

Graci

Francesca

et al. 2021

Plants

View full text Add to dashboard Cite

The constitution of heat tolerant F1 hybrids is a challenge to ensure high yield and good fruit quality in the global climate. In the present work, we evaluated 15 genotypes for yield-related traits highly affected by high temperatures (HT). This phenotypic analysis allowed to identify four parental genotypes showing promising yield performances under HT conditions. Two of these genotypes also exhibited good fruit quality traits. A molecular marker analysis was carried out for six resistance genes to pathogens mostly affecting tomatoes. This analysis evidenced the presence of a maximum of three resistant alleles in parental genotypes. Exploring single nucleotide polymorphisms (SNPs) revealed by two high-throughput genotyping platforms allowed identifying additional 12 genes potentially involved in resistance to biotic stress, to be further investigated. Following these considerations, 13 F1 hybrids were constituted combining the parental genotypes and then evaluated for multiple traits under HT conditions. By estimating a hybrid index based on yield performances, desirable quality and resistance gene, we identified seven hybrids showing the best performances. The promising results obtained in the present work should be confirmed by evaluating the best hybrids selected for additional years and environments before proposing them as novel commercial hybrids that could maintain high performances under HT conditions.

show abstract

Section: Molecular Screening For Resistance Genes In Parental Linesmentioning

confidence: 99%

Accelerating the Development of Heat Tolerant Tomato Hybrids through a Multi-Traits Evaluation of Parental Lines Combining Phenotypic and Genotypic Analysis

Olivieri

Graci

Francesca

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…Оценка устойчивости к фитофторозу растений томата путем инокуляции отделенных листьев подтвердила соответствие между наличием последовательности Ph-3-GLR/S и фенотипической резистентностью. Показано, что маркер Ph-3-GLR/S был более точным, чем TG328 и может служить высокоспецифичным и надежным кодоминантным маркером для селекции на наличие гена Ph-3 [42].…”

Section: Breeding Seed Production and Plant Biotechnologyunclassified

Molecular and genetic basis of tomato resistance to major fungal diseases

Maslova,

Shamshin,

Grosheva

et al. 2023

Ovoŝi Rossii

View full text Add to dashboard Cite

This review presents an analysis of the literature, which indicates the popularity of breeding methods in the fight against oomycete and fungal diseases of tomato: late blight (pathogen − Phytophthora infestans (Mont.) de Bary), fusarium wilt (pathogen − Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder and Hansen), early blight or alternariosis (pathogens − representatives of the genus Alternaria), cladosporiosis (pathogen−Cladosporium fulvum Cook.). Theoretical and practical achievements, the latest developments in genetics, molecular biology, plant physiology, microbiology are considered. Special attention is paid to modern information about the diversity of tomato pathogens, their morphological, genetic, physiological features, including racial composition; the presence of pathogen resistance genes in the gene pool of the culture under study, their mapping and the possibility of introduction into the genome from other sources; about the developed markers of target genes and loci of quantitative traits; the degree of associations between a molecular marker and a target gene; about the features of inheritance of the studied trait, as well as the possibility of pyramiding R-genes and QTL in one genotype. To accelerate and improve the efficiency of tomato breeding for resistance to pathogens, these issues are of great importance. Their study will strengthen the integration of modern biotechnology with the traditional breeding process, which is carried out by classical methods.

show abstract