Development of a hygiene standard for raw milk based on the Lumac ATP-bioluminescence method

Bell, Catherine Elizabeth; Bowles, Catherine; Toszeghy, M.J.K.; Neaves, P.

doi:10.1016/0958-6946(96)00007-6

Cited by 20 publications

(11 citation statements)

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“…ATP bioluminescent methods can be used for monitoring raw cow's milk quality from the point of view of somatic cell count and microbial count (Bell, Bowles, Toszeghy, & Neaves, 1996;Frundzhyan, Parkhomenko, Brovko, & Ugarova, 2008). ATP bioluminescence assay was also used for estimation of the microbial load on meat (Siragusa, Cutter, Dorsa, & Koohmaraie, 1995).…”

Section: Applicationsmentioning

confidence: 99%

Determination of microbial load for different beverages and foodstuff by assessment of intracellular ATP

Bottari

Santarelli

Neviani

2015

Trends in Food Science & Technology

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Section: Applicationsmentioning

confidence: 99%

Determination of microbial load for different beverages and foodstuff by assessment of intracellular ATP

Bottari

Santarelli

Neviani

2015

Trends in Food Science & Technology

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“…Therefore, the ATP-Bioluminescence technique that uses the MLS equipment can be a quick and auxiliary method of checking the microbiological quality of the UHT milk, because this technique provides results similar to the standard method (Brazil, 1997). Some literature (Siragusa et al, 1995;Bell et al, 1996;Costa, 2006;Simm et al, 2008;Luo et al, 2009) demonstrated representative variation of results when comparisons were made between mesophilic standard count and ATPBioluminescence. Other works (Pires et al, 2005;Costa et al, 2006;Vilar et al, 2008) reported techniques involving ATPBioluminescence that measure the ATP regardless of its origin (microbial or not), making the establishment of statistical correlations between the results in RLU and the aerobic mesophilic microorganisms count extremely difficult, since the relation between the microorganism number and the amount of ATP is not constant.…”

Section: Resultsmentioning

confidence: 99%

ATP-Bioluminescence as a method to evaluated microbiological quality of UHT milk

Cunha

Lage

Araújo

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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New approaches are needed to quickly indicate possible contamination of UHT milk, among them the technique of ATP-Bioluminescence. Therefore, the aim of this study was to compare the results of culture methods with the results of ATP-Bioluminescence technique of 102 UHT whole milk samples incubated at 48, 72, and 168 hours. UHT milk samples were analyzed for the presence of mesophilic and psychrotrophic aerobic microorganisms using Plate Count Agar (PCA), Brain-Heart Infusion (BHI) media and Petrifilm TM Aerobic Count (AC) plates. The ATP-Bioluminescence technique was applied through the Microbial Luminescent Screening (MLS) system. Significant correlations were found between counts of aerobic mesophilic microorganisms on PCA, Petrifilm TM AC, BHI and results of ATP bioluminescence technique (P≤0.05). The ATP-Bioluminescence technique had higher correlation with counting method in PCA than BHI media. At lower pass/fail limits of Relative Light Units (60, 50, 45 and 40 RLU), the number of samples identified as positive increased and statistically agreed with aerobic mesophilic microorganism counts (P>0.05). For the dairy industry, the ATP-Bioluminescence technique may become an important tool that assists the official methods to quickly monitor the microbiological quality of UHT milk though this will likely require a threshold below 150 RLU.Keywords: ATP-Bioluminescence, microbiology, milk, quality, UHT RESUMO Novos métodos são necessários para detectar de forma rápida a contaminação do leite UAT, entre eles, a técnica de ATP-Bioluminescência. Portanto, objetivou-se comparar os resultados de métodos de cultura tradicionais com os resultados de ATP-Bioluminescência de 102 amostras de leite UAT integral incubadas por 48, 72 e 168 horas. Os leites UAT foram analisados quanto à presença de microorganismos aeróbicos mesófilos e psicrotróficos usando os ágares PCA, BHI e placas Petrifilm TM AC. A técnica de ATP-Bioluminescência foi aplicada por meio do sistema Microbial Luminescent Screening (MLS). Significantes correlações foram obtidas entre as contagens de micro-organismos mesófilos

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“…Adenosine triphosphate bioluminescence is a common biochemical method for detecting bacterial and nonbacterial contamination and rapidly assessing hygienic quality in the dairy industry in small areas; however, ATP swab tests are not useful when large areas need inspection (Bell et al . ; Turner et al . ).…”

Section: Introductionmentioning

confidence: 96%

Formation of dairy fouling deposits on food contact surfaces

Fysun

Kern

Wilke

et al. 2019

Int J of Dairy Tech

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This study aimed to compare microbiological, biochemical and physical methods to quantify dairy fouling deposits. The influence of factors affecting dairy fouling formation is investigated by selected methods with respect to material type (polytetrafluoroethylene and stainless steel) and temperature (4 and 20 °C) for a defined time. The factors were investigated using nonmicrobiologically caused and microbiologically caused dairy deposits formed by UHT and pasteurised milk inoculated with Pseudomonas fragi. Both deposit types exhibited different adhesion behaviours. The highest positive correlation coefficient was found between biochemical and microbiological methods (0.932) obtained at both incubation temperatures after 24 and 48 h.

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Development of a hygiene standard for raw milk based on the Lumac ATP-bioluminescence method

Cited by 20 publications

References 1 publication

Determination of microbial load for different beverages and foodstuff by assessment of intracellular ATP

Determination of microbial load for different beverages and foodstuff by assessment of intracellular ATP

ATP-Bioluminescence as a method to evaluated microbiological quality of UHT milk

Formation of dairy fouling deposits on food contact surfaces

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