Development of a Linear Ion Trap Mass Spectrometer Capable of Analyzing Megadalton MALDI Ions

“IgG’s: contending with aggregating circumstances”

“…19 In addition, MALDI TOF MS that employs a high MW dynode detector is also effective for directly and quantitatively assaying antibody aggregates, including mega-Dalton IgM species. 20 As described above, protein aggregation (including IgG's) frequently involves conformational shifts that expose hydrophobic domains. 5,8,19 This alters protease accessibility, and has led to the development of limited proteolysis as an aggregate assay that can additionally inform on shifts in protease accessibility within specific domains.…”