Abstract:Aim. The aim of this study was to develop a loop-mediated isothermal amplification (LAMP) assay for African swine
fever virus (ASFV) detection. Methods. Primer design was performed using publicly available full genome sequences
of ASFV. A panel of heterologous DNA samples and reference ASFV DNA samples were used for the assay specificity
testing. The limit of detection (LOD) was assessed using purified and quantified serial dilution of the amplified target
sequence. LAMP product detection was performed via gel… Show more
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