Development of a mandarin fish Siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (ISKNV)

Dong, Chuanfu; Weng, Shaoping; Shi, Xiujuan; Xu, Xiaopeng; Shi, Nan; He, Jianguo

doi:10.1016/j.virusres.2008.04.004

Cited by 155 publications

(128 citation statements)

References 33 publications

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“…The establishment of healthy fish cell lines is essential for isolation, identification and characterization of infectious viruses from fishes (Parameswaran et al, 2007;Dong et al, 2008). Although a large number of cell lines (more than 160) have been established, relatively only a few cell lines were derived from marine fish (Fryer and Lannan, 1994;Chen et al, 2003;Bryson et al, 2006;Buonocore et al, 2006;Parameswaran et al, 2007;Zhou et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Characterization of two grouper Epinephelus akaara cell lines: Application to studies of Singapore grouper iridovirus (SGIV) propagation and virus–host interaction

et al. 2009

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Section: Introductionmentioning

confidence: 99%

Characterization of two grouper Epinephelus akaara cell lines: Application to studies of Singapore grouper iridovirus (SGIV) propagation and virus–host interaction

et al. 2009

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“…The MFF-1 and KCF-1 cell lines were developed and characterized from mandarin fish fry and caudal fin of a young koi, respectively, and kept in our laboratory (Dong et al 2008(Dong et al , 2011. Both MFF-1 and KCF-1 cells were grown in complete DMEM supplemented with 10 % fetal bovine serum (Gibco, Life Technologies, Carlsbad, CA, USA) at 25°C.…”

Section: Cell Lines and Megalocytiviral Strainsmentioning

confidence: 99%

“…Very few piscine cell lines are susceptible to megalocytivirus (Dong et al 2008;Imajoh et al 2007), unlike ranaviruses, which propagate well in a very broad range of cell lines (Pham et al 2012;Zhang et al 1999). In most cases, normal piscine cell lines only support the propagation of megalocytivirus in very limited number of viral passages.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous reports, an epithelial-like cell line called MFF-1 was developed and was shown to be highly susceptible to both freshwater-borne and marine-borne megalocytiviruses (Dong et al 2008(Dong et al , 2010Ma et al 2012;Shuang et al 2013). However, MFF-1 was suspected to be a naturally transformed cell line due to its high variability of chromosomal distribution (Dong et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…However, MFF-1 was suspected to be a naturally transformed cell line due to its high variability of chromosomal distribution (Dong et al 2008). Development of more susceptible cell lines is necessary to understand the infection mechanism of megalocytiviruses and for a broader screening of suitable cellular substrates for cell-cultured vaccines.…”

Section: Introductionmentioning

confidence: 99%

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Cloning of a new fibroblast cell line from an early primary culture from mandarin fish (Siniperca chuatsi) fry for efficient proliferation of megalocytiviruses

et al. 2013

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Megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. However, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. In this study, a new fibroblast-like cell line was established from an early primary culture from mandarin fish fry by a single cell cloning and was designated as MFF-8C1. The MFF-8C1 cells grow well in Dulbecco's modified Eagle's medium supplemented with 10 % fetal bovine serum and had been subcultured more than 60 passages since the initial recovery culture in October 2009. Chromosomal analysis revealed that 91 % of the MFF-8C1 cells maintained a normal diploid chromosome number (2n = 48) in the 46th passage. Infection experiments showed that both freshwater-borne and marine-borne megalocytiviruses induce severe cytopathic effects in infected MFF-8C1 cells characterized by the rounding and enlargement of cells, which are highly consistent with the previous description of the infection in other susceptible cells with megalocytivirus. Megalocytivirus infections were further confirmed by a transmission electron microscopy. Furthermore, the MFF-8C1-cultured megalocytiviral suspension was highly virulent to infected mandarin fish. In summary, a new fibroblast cell line from mandarin fish fry that was highly permissive to megalocytiviruses was established. The MFF-8C1 cell line is a promising cellular substrate candidate for cell-cultured vaccine production of megalocytivirus.

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Designing cell lines for viral vaccine production: Where do we stand?

Genzel

2015

Biotechnology Journal

101

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Established animal cells, such as Vero, Madin Darby canine kidney (MDCK) or chicken embryo fibroblasts (CEFs), are still the main cell lines used for viral vaccine production, although new "designer cells" have been available for some years. These designer cell lines were specifically developed as a cell substrate for one application and are well characterized. Later screening for other possible applications widened the product range. These cells grow in suspension in chemically defined media under controlled conditions and can be used for up to 100 passages. Scale-up is easier and current process options allow cultivation in disposable bioreactors at cell concentrations higher than 1 × 10(7) cells/mL. This review covers the limitations of established cell lines and discusses the requirements and screening options for new host cells. Currently available designer cells for viral vaccine production (PER.C6, CAP, AGE1.CR, EB66 cells), together with other new cell lines (PBS-1, QOR/2E11, SogE, MFF-8C1 cells) that were recently described as possible cell substrates are presented. Using current process knowledge and cell line development tools, future upstream processing could resemble today's Chinese hamster ovary (CHO) cell processes for monoclonal antibody production: small scale bioreactors (disposable) in perfusion or fed-batch mode with cell concentrations above 1 × 10(8) cells/mL.

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Development of a mandarin fish Siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (ISKNV)

Cited by 155 publications

References 33 publications

Characterization of two grouper Epinephelus akaara cell lines: Application to studies of Singapore grouper iridovirus (SGIV) propagation and virus–host interaction

Characterization of two grouper Epinephelus akaara cell lines: Application to studies of Singapore grouper iridovirus (SGIV) propagation and virus–host interaction

Cloning of a new fibroblast cell line from an early primary culture from mandarin fish (Siniperca chuatsi) fry for efficient proliferation of megalocytiviruses

Designing cell lines for viral vaccine production: Where do we stand?

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