Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit

Garcia-Martinez, G.; Bustabad, E. A.; Perrot, Hubert; Gabrielli, C.; Bucur, Bogdan; Lazerges, Mathieu; Rose, Daniel; Rodríguez-Pardo, L.; Fariña, José; Compère, Chantal; Arnau, Antonio

doi:10.3390/s110807656

Cited by 64 publications

(35 citation statements)

References 17 publications

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“…3 together with finite element analysis (FEA) predictions, where it was assumed that the additional mass added was evenly distributed on the resonators' surface. It can be seen that the FBARs exhibit a greater responsitivity, R m =~6.9 kHz-pg" 1 , than the SMRs, 7? m =~5.1 kHz-pg" 1 .…”

Section: B Comparison Of the Mass Responsitivities Of Fbars And Smrsmentioning

confidence: 99%

“…It can be seen that the FBARs exhibit a greater responsitivity, R m =~6.9 kHz-pg" 1 , than the SMRs, 7? m =~5.1 kHz-pg" 1 . It is worthwhile noting that the precise values of responsitivity depend in part on the resonators' size and design, and therefore these specific values can not be taken as an universal FBAR nor SMR response to mass loadings.…”

Section: B Comparison Of the Mass Responsitivities Of Fbars And Smrsmentioning

confidence: 99%

“…Bulk acoustic wave (BAW) resonators, such as the quartz crystal microbalance (QCM), are nowadays routinely used in gravimetric sensing applications [1][2][3]. Historically, the emergence of the quartz crystal as a microbalance can be traced back to Sauerbrey [4], who realised that a resonating quartz crystal could be used for the micro gravimetric measurement of sputtered thin films.…”

Section: Introductionmentioning

confidence: 99%

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Experimental comparison of FBARs and SMRs responsitivities to mass loadings

Garcia‐Gancedo

Pedrós²,

Iborra³

et al. 2012

2012 IEEE International Ultrasonics Symposium

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-The utilisation of thin film technology to develop film bulk acoustic resonators (FBARs) and solidly mounted resonators (SMRs), offers great potential to outperform the sensitivity and minimum detection limit of gravimetric sensors. Up to now, the choice between FBARs and SMRs depends primarily on the users' ability to design and fabricate Bragg reflectors and/or membranes, because neither of these two types of resonators has been demonstrated to be superior to the other. In the work reported here, it is shown that identically designed FBARs and SMRs resonating at the same frequency exhibit different responsitivities, R m , to mass loadings, being the FBARs more responsive than the SMRs. For the specific device design and resonant frequency (~2 GHz) of the resonators presented, FBARs' mass responsitivity is ~20% greater than that of SMRs, and although this value should not be taken as universal for all possible device designs, it clearly indicates that FBAR devices should be favoured over SMRs in gravimetric sensing applications.

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Section: B Comparison Of the Mass Responsitivities Of Fbars And Smrsmentioning

confidence: 99%

Section: B Comparison Of the Mass Responsitivities Of Fbars And Smrsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Experimental comparison of FBARs and SMRs responsitivities to mass loadings

Garcia‐Gancedo

Pedrós²,

Iborra³

et al. 2012

2012 IEEE International Ultrasonics Symposium

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“…DNA sensors have been used to detect DNA (Rogers et al, 2001;Wang et al, 1998), proteins (Ban et al, 2004;Leung et al, 2012), and even small molecules/ions (Li and Lu, 2000;Zhang et al, 2014) in the form of optical Rogers et al, 2001;Vo-Dinh et al, 2001), electrochemical (Kinsella and Ivanisevic, 2007;Odenthal and Gooding, 2007;Wang, 2006), or mass-sensitive (Garcia-Martinez et al, 2011;Mannelli et al, 2005) for a variety of biological applications. One particular example can be using DNA probes to differentiate healthy, gingivitis, and periodontitis subjects using DNA-DNA hybridization techniques (Palys et al, 1998).…”

Section: Dna As a Biomarker Binding Platformmentioning

confidence: 99%

Materiomics for Oral Disease Diagnostics and Personal Health Monitoring: Designer Biomaterials for the Next Generation Biomarkers

Zhang

Wang

Khalili

et al. 2016

OMICS: A Journal of Integrative Biology

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We live in exciting times for a new generation of biomarkers being enabled by advances in the design and use of biomaterials for medical and clinical applications, from nano-to macro-materials, and protein to tissue. Key challenges arise, however, due to both scientific complexity and compatibility of the interface of biology and engineered materials. The linking of mechanisms across scales by using a materials science approach to provide structure-process-property relations characterizes the emerging field of 'materiomics,' which offers enormous promise to provide the hitherto missing tools for biomaterial development for clinical diagnostics and the next generation biomarker applications towards personal health monitoring. Put in other words, the emerging field of materiomics represents an essentially systematic approach to the investigation of biological material systems, integrating natural functions and processes with traditional materials science perspectives. Here we outline how materiomics provides a game-changing technology platform for disruptive innovation in biomaterial science to enable the design of tailored and functional biomaterials-particularly, the design and screening of DNA aptamers for targeting biomarkers related to oral diseases and oral health monitoring. Rigorous and complementary computational modeling and experimental techniques will provide an efficient means to develop new clinical technologies in silico, greatly accelerating the translation of materiomics-driven oral health diagnostics from concept to practice in the clinic.

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“…QCM-D technique has been used widely in different studies, i.e: cellular characterization [25,26,[91][92][93], protein detection [27,[94][95][96][97][98] and DNA detection [99][100][101][102][103][104], and viscosity analysis of different substances [37,46,67,100,[105][106][107][108].…”

Section: Impulse Excitation and Decay Methodsmentioning

confidence: 99%

Diseño y evaluación de un biosensor basado en resonadores de cristal de cuarzo (QCR) para caracterizar muestras biológicas relacionadas con enfermedades artríticas

Ahumada¹,

Armando²

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IVResumen de la tesis de Luis Armando Carvajal Ahumada, presentada como requisito parcial para la obtención del grado de DOCTOR en INGENIERÍA BIOMÉDICA. Madrid, 03 de Abril de 2017 DISEÑO Y EVALUACIÓN DE UN SENSOR BASADO EN RESONADORES DE CRISTAL DE CUARZO (QCR) PARA CARACTERIZAR MUESTRAS BIOLÓGICAS RELACIONADAS CON LA DIAGNOSIS DE ENFERMEDADES ARTRÍTICASResumen:Los resonadores de cristal de cuarzo son dispositivos ampliamente utilizados como biosensores debido a su alta sensibilidad ante fenómenos físico-químicos que ocurren en su superficie, a la reproducibilidad de las mediciones y por ser transductores fáciles de utilizar. Sauerbrey[1] fue el primero en demostrar que el resonador cristal de cuarzo puede ser utilizado como una microbalanza útil para caracterizar pequeñas deposiciones de masa en el electrodo del cristal. Sauerbrey demostró que, si se deposita una delgada capa de material rígido sobre el cristal de cuarzo, tanto el cristal como el material depositado vibrarán a la misma frecuencia y se generará un cambio en la frecuencia de resonancia inicial del cristal. Específicamente, la frecuencia de resonancia disminuye al aumentar la cantidad de masa depositada.El cristal de cuarzo también puede operar en medio fluidos. En este caso además del desplazamiento en frecuencia del cristal, se genera una pérdida significativa en el factor de calidad del resonador. Para fluidos Newtonianos (viscosidad constante), Kanazawa y Gordon[2] demostraron que el desplazamiento en frecuencia que sufre el resonador así como el cambio en el ancho de la curva de admitancia, depende de la densidad y la viscosidad del líquido. Este fenómeno permite utilizar los resonadores de cristal de cuarzo como pequeños viscosímetros siempre que se conozca la densidad del fluido a analizar.En el caso de deposición de fluidos viscoelásticos, la medición del cambio en la frecuencia de resonancia del cristal no es suficiente para obtener el valor de viscosidad del fluido. Por tanto, se requiere de la medición de una segunda variable (mitad del ancho de media banda -Γ) para obtener el valor correcto de la viscosidad del fluido depositado.[3] Para fluidos pseudoplásticos (viscosidad aparente dependiente de la velocidad de cizallamiento aplicada), se requiere de un modelo matemático que explique el comportamiento de la viscosidad del fluido a distintas velocidades de cizallamiento. En particular para el análisis de muestras con este comportamiento, se ha elegido el modelo Rouse ya que explica con suficiente detalle el comportamiento pseudoplástico y resulta simple de aplicar a las medidas experimentales.En este trabajo se propone el diseño de un biosensor basado en un resonador de cristal de cuarzo (QCR) para la caracterización de muestras biológicas que emulan el comportamiento del líquido sinovial.El líquido sinovial es una mezcla de plasma dializado y ácido hialurónico con comportamiento pseudoplástico. Su principal función es la de actuar como lubricante en las articulaciones V manteniendo al mínimo la fricción entre los huesos, especialment...

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Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit

Cited by 64 publications

References 17 publications

Experimental comparison of FBARs and SMRs responsitivities to mass loadings

Experimental comparison of FBARs and SMRs responsitivities to mass loadings

Materiomics for Oral Disease Diagnostics and Personal Health Monitoring: Designer Biomaterials for the Next Generation Biomarkers

Diseño y evaluación de un biosensor basado en resonadores de cristal de cuarzo (QCR) para caracterizar muestras biológicas relacionadas con enfermedades artríticas

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