Development of a method for simultaneous screening of four natural-derived steroids and their analogues used as dietary supplements via liquid chromatography-quadrupole-time of flight mass spectrometry and liquid chromatography-tandem mass spectrometry

Abstract

Natural-derived steroids and their analogues are present in various plants and insects. To minimize the chance of missing a positive doping test and avoiding potentially serious health problems, adequate screening methods are necessary for the detection of a wide range of natural-derived steroids and their analogues in dietary supplements. In this study, an accurate and simple liquid-chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to determine and quantify the natural-derived steroids and their analogues according to the International Conference on Harmonization of technical

Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition

“…Turkesterone is a PE, with 27 carbon atoms and seven OH-groups; it is considered that the OH groups at C-20 and C-11 are responsible for the anabolic effects [28,215]. Turkesterone is considered to possess an anabolic effect, and it is used as DS by athletes, instead of anabolic steroids [28,30]. It is found in some endemic plants, such as A. turkestanica, R. carthamoides, Triticum aestivum L., Vitex scabra, etc.…”