Development of a method for the determination of polyamines including N-acetylated forms in human saliva via benzoylation and gas chromatography-mass spectrometry

Peña, Javier; Laespada, María Esther Fernández; Pinto, Carmelo Garcı́a; Pavón, José Luis Pérez

doi:10.1016/j.chroma.2021.462278

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…In general, polyamines are analyzed in samples using chromatographic methods (Cho & Kang, 2018; Coradduzza et al, 2021; Liu et al, 2012; Novita Sari et al, 2021; Peña et al, 2021; Teti et al, 2002). As polyamines are polycations in solution, they can be easily analyzed by capillary electrophoresis (CE; Daniel et al, 2015; Elbashir et al, 2014; Igarashi et al, 2021; Sugimoto et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the stability of polyamines in human serum at different storage temperatures using capillary electrophoresis with indirect ultraviolet detection

Silva

Nishida

Calixto

et al. 2023

Biomedical Chromatography

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Polyamines are low molecular weight compounds that are present in all living organisms. They are related to the pathological processes, and have been studied as biomarkers for tumor progression, being analyzed in patients' biological fluids. However, polyamines can undergo degradation in serum samples, depending on storage conditions, which impairs their quantification in these matrices. In this work, capillary electrophoresis using indirect ultraviolet detection has been developed and applied to evaluate the stability of polyamines [cadaverine (Cad), putrescine (Put), spermine (Spm), and spermidine (Spd)] in human serum at different storage temperatures. By using this method, Cad, Put, Spm, and Spd were separated in less than 4 min. The range of the correlation coefficients was 0.993-0.998. The corresponding limits of detection and quantification were as follows (in mg L -1 ): Spm: 0.209 and 0.697; Spd: 0.165 and 0.549; Put: 0.189 and 0.632; Cad: 0.125 and 0.417. Besides, the coefficient of variation was lower than 1% for all analytes and the recovery was 92%-110%. The method was successfully applied for polyamines spiked in human serum samples from healthy people. The results showed that the degradation of polyamines was lower in samples stored in a freezer (À20 C).

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Section: Introductionmentioning

confidence: 99%

Evaluation of the stability of polyamines in human serum at different storage temperatures using capillary electrophoresis with indirect ultraviolet detection

Silva

Nishida

Calixto

et al. 2023

Biomedical Chromatography

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“…Therefore, they have been widely used in military, medical, transportation, environmental protection, quality inspection, anti-counterfeiting, home, and other fields. [7][8][9][10] However, there are still some issues with the commercially available gas sensors, such as poor selectivity and stability. Further improvement of various performance indicators of gas sensors, as well as new materials for gas sensing and new gas sensors developed, are receiving increasing attention.…”

Section: Introductionmentioning

confidence: 99%

“Visualization” Gas—Gas Sensors Based on High Performance Novel MXenes Materials

Wang,

Kuai

et al. 2023

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The detection of toxic, harmful, explosive, and volatile gases cannot be separated from gas sensors, and gas sensors are also used to monitor the greenhouse effect and air pollution. However, existing gas sensors remain with many drawbacks, such as lower sensitivity, lower selectivity, and unstable room temperature detection. Thus, there is an imperative need to find more suitable sensing materials. The emergence of a new 2D layered material MXenes has brought dawn to solve this problem. The multiple advantages of MXenes, namely high specific surface area, enriched terminal functionality groups, hydrophilicity, and good electrical conductivity, make them among the most prolific gas‐sensing materials. Therefore, this review paper describes the current main synthesis methods of MXenes materials, and focuses on summarizing and organizing the latest research results of MXenes in gas sensing applications. It also introduces the possible gas sensing mechanisms of MXenes materials on NH3, NO2, CH3, and volatile organic compounds (VOCs). In conclusion, it provides insight into the problems and upcoming challenges of MXenes materials for gas sensing.

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Systematic evaluation of benzoylation for liquid chromatography-mass spectrometry analysis of different analyte classes

Lenhart,

Booth,

Simcox

et al. 2024

Journal of Chromatography A

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Development of a method for the determination of polyamines including N-acetylated forms in human saliva via benzoylation and gas chromatography-mass spectrometry

Cited by 5 publications

References 35 publications

Evaluation of the stability of polyamines in human serum at different storage temperatures using capillary electrophoresis with indirect ultraviolet detection

Evaluation of the stability of polyamines in human serum at different storage temperatures using capillary electrophoresis with indirect ultraviolet detection

“Visualization” Gas—Gas Sensors Based on High Performance Novel MXenes Materials

Systematic evaluation of benzoylation for liquid chromatography-mass spectrometry analysis of different analyte classes

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