Development of a method for the determination of ultra‐trace level mercury in adipose tissue by cold vapour atomic fluorescence spectrometry

Levine, Keith; Fernando, Reshan; Lang, Michelle; Essader, Amal; Handy, Robert W.; Collins, Bradley J.

doi:10.1155/s1463924600000146

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…However, in a case-control study, Hg was detected in subcutaneous and visceral fat obtained from women undergoing abdominal surgery for uterine leiomyoma tumors, and in subcutaneous fat of women receiving liposuction for cosmetic reasons (average values: 5.9–13 ng Hg/g fat, Qin et al 2010). Hg has also been detected in the adipose tissue of rats (<1–5.6 ng/g, Levine et al 2000) and marine mammals (3–190 ng/g, Wagemann et al 1998). Adipose tissue contains adipocytes, which are comprised mainly of lipid droplets (~95%), as well as stromal vascular cells with less lipid content (Lee et al 2013).…”

Section: Discussionmentioning

confidence: 99%

The influence of obesity on blood mercury levels for U.S. non-pregnant adults and children: NHANES 2007–2010

Rothenberg

Korrick

Fayad

2015

Environmental Research

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Background In animal studies obesity is associated with higher blood and tissue mercury concentrations; however human studies are lacking. Although the mechanism underlying this association is uncertain, obesity may alter the metabolism and distribution of methylmercury. Objectives We determined whether obesity influenced blood mercury levels, the majority of which was methylmercury, for U.S. non-pregnant adults (≥20 years) and children (2–19 years) after controlling for methylmercury intake through fish and shellfish consumption, and other confounders. Methods We completed secondary data analysis of the National Health and Nutrition Examination Survey (NHANES) (2007–2010) for participants who consumed fish/shellfish within 24 hours of blood collection for mercury analysis. Weighted least squares regression models related blood mercury levels (the dependent variable) to methylmercury exposure (μg) from fish consumed in the previous 24 hours, body mass index (BMI) (for adults), BMI z-scores (for children), and other confounders. Results: In adjusted models, blood mercury levels were inversely correlated with BMI for adults [β, 95% confidence interval (CI) = −0.54 (−0.90, −0.18)]. For children, blood mercury levels were inversely correlated with BMI z-scores but the trend was not significant [β (95% CI) = −0.016 (−0.066, 0.035)]. When obese adults or children were compared with those who were overweight/normal weight, blood mercury averaged 22% lower for obese adults (95% CI: −33%, −8.2%), while blood mercury did not differ significantly for obese children [β (95% CI) = −1.7% (−31%, +39%)]. Conclusions After adjusting for the main, if not exclusive, exogenous source of methylmercury exposure (through fish/shellfish intake) and other confounders, our results support potential changes in the metabolism, distribution or excretion of methylmercury with increasing BMI (for adults).

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Section: Discussionmentioning

confidence: 99%

The influence of obesity on blood mercury levels for U.S. non-pregnant adults and children: NHANES 2007–2010

Rothenberg

Korrick

Fayad

2015

Environmental Research

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“…The procedure was also applied to the analysis of biological, environmental and general merchandise materials. For the analysis of adipose tissue, 272 samples were first lyophilized to facilitate homogenization and the accurate weighing of 50 mg samples. Closed-vessel, microwave-assisted digestion with HNO 3 -H 2 SO 4 was followed by oxidation of the liberated mercury species with potassium bromate-bromide reagent.…”

Section: Cadmiummentioning

confidence: 99%

Atomic Spectrometry Update. Advances in atomic emission, absorption, and fluorescence spectrometry, and related techniques

Evans

Dawson²,

Fisher

et al. 2001

J. Anal. At. Spectrom.

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“…The most commonly employed mode of instrumental analysis for this purpose has been cold vapor atomic absorption spectrometry (CVAAS). Cold vapor atomic fluorescence spectrometry (CVAFS) has been employed for the determination of mercury in adipose, [18] oyster, [19] fecal, urine, milk, and kidney, [20] and blood and plasma [21] tissue samples. [13] Although mercury has also been determined in human brain tissue by neutron activation analysis (NAA) [14] and by inductively coupled plasma mass spectrometry (ICP-MS), [15] both of these techniques are cost-prohibitive for many laboratories.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Method for the Determination of Mercury in Small Rat Brain Tissue Samples by Cold Vapor Atomic Fluorescence Spectrometry

et al. 2002

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A rugged, sensitive, semi-automated method for the determination of trace-level mercury in small rat brain tissue samples by cold vapor atomic fluorescence spectrometry (CVAFS) is described. Small tissue aliquots (100 mg) are digested for 6-8 h at an elevated temperature in a mixture of nitric and sulfuric acids. Following digestion, samples are oxidized with potassium bromate/bromide at room temperature and treated with ANALYTICAL LETTERS a continuous flow of stannous chloride to generate mercury vapor. The experimentally determined method quantitation limit (MQL) in the rat brain matrix is 10 pg Hg/mL, which corresponds to 1.5 ng Hg/g brain tissue. Replicate matrix spike preparations (n ¼ 4) at this level were determined with a high level of accuracy (89.8%) and precision (6.9% RSD). Replicates (n ¼ 36) of a certified reference material (DOLT-2, trace metals in dogfish liver) were prepared and analyzed over a four year period in order to assess the ruggedness and accuracy of the method. The average recovery for mercury in this material was 99.9% of the certified level.

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Development of a method for the determination of ultra‐trace level mercury in adipose tissue by cold vapour atomic fluorescence spectrometry

Abstract: A method for the determination of total mercury in rat adipose

Cited by 5 publications

References 10 publications

The influence of obesity on blood mercury levels for U.S. non-pregnant adults and children: NHANES 2007–2010

The influence of obesity on blood mercury levels for U.S. non-pregnant adults and children: NHANES 2007–2010

Atomic Spectrometry Update. Advances in atomic emission, absorption, and fluorescence spectrometry, and related techniques

Development and Validation of a Method for the Determination of Mercury in Small Rat Brain Tissue Samples by Cold Vapor Atomic Fluorescence Spectrometry

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