2015
DOI: 10.3139/113.110367
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Development of a Method for the Analysis of Microbial Load Reduction Factors on Dishes Cleaned by Hand and by Machine

Abstract: The aim of this study was to develop arepeatable method for the measurement of microbial load reduction factors after hand and machine dishwashing processes that meet the requirementsa ssociated with consumer studies, based on non-pathogen test strains. Microorganisms were isolated from tableware, identified and cultured for this purpose, mixed with soiling agents and lyophilised for storage purposes. Detailed instructionsfor ambient conditions and disinfection, and preparation and applicationo f soiling agent… Show more

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Cited by 6 publications
(3 citation statements)
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“…They were rehydrated for 30 min in 0·5 ml tryptic soy broth (TSB, Merck KGaA, Darmstadt, Germany). For M. luteus DSM 28269, an actively growing culture on tryptic soy agar (TSA, Merck KGaA) was received from the University of Bonn, who first isolated the micro‐organism from dishwashers (Berger et al ).…”
Section: Methodsmentioning
confidence: 99%
“…They were rehydrated for 30 min in 0·5 ml tryptic soy broth (TSB, Merck KGaA, Darmstadt, Germany). For M. luteus DSM 28269, an actively growing culture on tryptic soy agar (TSA, Merck KGaA) was received from the University of Bonn, who first isolated the micro‐organism from dishwashers (Berger et al ).…”
Section: Methodsmentioning
confidence: 99%
“…Therefore, four apathogenic bacterial strains were tested: Micrococcus luteus (M. luteus), Lysinibacillus macroides (L. macroides) [8], Pseudomonas fluorescens (P. fluorescens) [17], and Geobacillus atrophaeus (G. atrophaeus).…”
Section: Initial Selection Of Test Strainsmentioning
confidence: 99%
“…Studies have already been conducted that tested isolated bacterial strains within manual and automatic dishwashing experiments in the domestic area [8,9]. There are different standard guidelines regarding commercial dishwashers, such as the standards of the German Institute for Standardization DIN 10510, DIN 10511, DIN 10512 and the DIN 10522, which describe type-specific methods of testing [10 -14].…”
Section: Introductionmentioning
confidence: 99%