Development of a Method for Fucoxanthin Production Using the Haptophyte Marine Microalga Pavlova sp. OPMS 30543

Abstract

The natural pigment fucoxanthin has attracted global attention because of its superior antioxidant properties. The haptophyte marine microalgae Pavlova spp. are assumed to be promising industrial fucoxanthin producers as their lack of a cell wall could facilitate the commercialization of cultured cells as a whole food. This study screened promising Pavlova strains with high fucoxanthin content to develop an outdoor cultivation method for fucoxanthin production. Initial laboratory investigations of P. pinguis NBRC 102807, P. lutheri NBRC 102808, and Pavlova sp. OPMS 30543 identified OPMS 30543 as having the highest fucoxanthin content. The culture conditions were optimized for OPMS 30543. Compared with f/2 and Walne's media, the use of Daigo's IMK medium led to the highest biomass production and highest fucoxanthin accumulation. The presence of seawater elements in Daigo's IMK medium was necessary for the growth of OPMS 30543. OPMS 30543 was then cultured outdoors using acrylic pipe photobioreactors, a plastic bag, an open tank, and a raceway pond. Acrylic pipe photobioreactors with small diameters enabled the highest biomass production. Using an acrylic pipe photobioreactor with 60-mm diameter, a fucoxanthin productivity of 4.88 mg/L/day was achieved in outdoor cultivation. Thus, this study demonstrated the usefulness of Pavlova sp. OPMS 30543 for fucoxanthin production in outdoor cultivation.

Fucoxanthin Production of Microalgae under Different Culture Factors: A Systematic Review

“…They were assessed further for eligibility based on the defined criteria: (1) inclusion of studies, specifically fucoxanthin production of microalgae involving fucoxanthin content or both growth (biomass concentration) and fucoxanthin content under different culture conditions, and the (2) exclusion of studies involving environmental studies (studies utilised fucoxanthin to analyse the microalgae community in a particular habitat). Finally, 28 articles were included in the present systematic review [ 4 , 5 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. The information from these articles was extracted and is summarised in Table 1 .…”

“…Several studies compared the fucoxanthin content of microalgae and macroalgae [ 25 , 39 , 40 ]. The fucoxanthin content of the macroalgae ranged from 0.001 to 0.356% DW [ 16 , 25 ]. Microalgae demonstrated that their fucoxanthin content is up to 100 times higher than that of macroalgae.…”

“…Meanwhile, four different nitrogen sources (200 mg/L) such as sodium nitrate (NaNO 3 ), potassium nitrate (KNO 3 ), urea (CO(NH 2 ) 2 ) and ammonium chloride (NH 4 Cl) in the Daigo’s IMK medium were tested on the biomass concentration and fucoxanthin production of Pavlova sp. OPMS 30543 [ 16 ]. Media consisting of KNO 3 boosted the highest biomass concentration (1.8 g/L), while the lowest biomass concentration (0.82 g/L) was obtained using NH 4 Cl as a nitrogen source.…”

Anti-Inflammatory and Anticancer Effects of Microalgal Carotenoids

“…(Haptophyta), Odontella aurita [54,55], Nitzschia laevis (formerly Nitzschia amabilis) [56], and Chaetoceros neogracili (formerly Chaetoceros gracilis) (Bacillariophyta), the coccolithophore Pleurochrysis carterae (Haptophyta, Coccolithophyceae) [57], Phaeodactylum tricornutum (Bacillariophyta) [46], and the microalga strain Pavlova sp. OPMS 30543 (Haprophyta) [58].…”

High-Value Compound Induction by Flashing Light in Diacronema Lutheri and Tetraselmis Striata Ctp4

“…Furthermore, the biochemical profile is rich in lipids containing eicosapentaenoic acid (EPA) and the carotenoids lutein and β-carotene, which are important compounds for nutraceutical applications (Schüler et al, 2021). On the other hand, the haptophyte Diacronema lutheri (formerly known as Pavlova lutheri) is less studied for industrial production, although being promising strains for aquafeeds and human nutrition due to high contents of EPA and docosahexaenoic acid (DHA) and pigments such as fucoxanthin, diatoxanthin or β-carotene (Guihéneuf and Stengel, 2017;Tibbetts et al, 2019;Kanamoto et al, 2021).…”