Development of a micropropagation protocol for Malus orientalis using axillary buds

Amirchakhmaghi, Narjes; Hosseinpour, Batool; Yousefzadeh, Hamed

doi:10.1007/s11627-019-09992-4

Cited by 8 publications

(2 citation statements)

References 36 publications

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“…In the present study, the regeneration from GGBs of A. costularis was effectively inhibited by adding high concentration of cytokinin (2.0 mg/l TDZ), and the inhibition was released by culturing GGB on the medium without cytokinin (Table 3), which con rmed cytokinins controlling GGB organogenesis rstly proposed by Higuchi et al (1987). Different from the role of cytokinins in GGB system of fern plants, cytokinin TDZ effectively induced protocorm-like body (PLB) formation from root tips of Doritaenopsis (Park et al, 2003), cytokinin BA was bene cial to the shoot multiplication of Malus orientalis (Amirchakhmaghi et al 2019). Although the resurrection plant Selaginella pulvinata belongs to fern-allies, not GGBs but adventitious buds were induced by culturing the sterilized frond tips on the media containing various concentrations of BAP or TDZ (Yu et al 2021b).…”

Section: Discussionmentioning

confidence: 64%

In vitro propagation and long-term observation of acclimated plants in endangered tree fern Alsophila costularis

Song

Wang

et al. 2022

Plant Cell Tiss Organ Cult

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Alsophila costularis Barker (Cyathea costularis), an endangered tree fern with tree-like erect stem, attracts gardening enthusiasts as a special ornamental plant. In vitro propagation can be advantageous for germplasm conservation and commercial application of A. costularis. Here, we described in vitro propagation of A. costularis via spore culture and green globular bodies (GGBs) system, as well as the long-term observation of acclimated plants regenerated from GGBs. In spore culture, the low concentration of mineral salt (1/8 MS) was bene cial for sporophyte formation on gametophytes, but sporophytes per conical ask was only 8 plantlets. In GGB system, cytokinin thidiazuron (TDZ) was essential for GGB induction and multiplication. The maximum of GGB induction frequency (93.33%) was obtained on 1/2MS medium with 2.0 mg/l TDZ by using juvenile sporophytes as explants, and the same medium was optimal for GGB multiplication. 1/4 MS supplemented with 0.1% (w/v) activated carbon (AC) was appropriate for plantlet regeneration from GGB, GGB differentiation frequency was 100%, and 42.40 plantlets could be regenerated from one piece of GGBs. The maximum of plantlet height (4.64 cm) was obtained on 1/2 MS with 0.1% (w/v) AC. After 6 years of acclimatization cultivation for plantlets regenerated from GGBs, plants in the plastic pots with diameter of 60 cm showed the excellent vegetative and reproductive growth, and the mature spores of these plants could produce sporophytes. Morphological and histological observation demonstrated that A. costularis GGBs was a green structure consisting of multiple single GGBs with hair-like structures. One single GGB could develop into one plantlet.* Plantlet regeneration e ciency indicates the number of plantlets regenerated from one piece of GGBs, and the initial diameter and fresh weight of GGBs was 3.0 mm and 5.0 mg, respectively.

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Section: Discussionmentioning

confidence: 64%

In vitro propagation and long-term observation of acclimated plants in endangered tree fern Alsophila costularis

Song

Wang

et al. 2022

Plant Cell Tiss Organ Cult

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“…Furthermore, regenerating transgenic lines successfully requires micropropagation and determines if a transformation protocol is effective in plants ( Aroonpong, Chang 2015;Teixeira da Silva et al 2019). Multiple studies on apple micropropagation have been reported (Modgil et al 1999;Sicurani et al 2001;Nagy et al 2005;Li et al 2014;Amirchakhmaghi et al 2019). Different explants in the apple regeneration have been selected and studied, such as the axillary bud, thin cell layers, leaf shoot apices, stem cuttings or shoot tips (Yepes, Aldwinckle 1994;Caboni et al 2000;Dobránszki, Teixeira da Silva 2010;Modgil et al 2012).…”

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confidence: 99%

Establishment of an efficient micropropagation system in enhancing rooting efficiency via stem cuttings of apple rootstock M9T337

Shi¹,

Dong²,

Song³

et al. 2021

Hort. Sci. (Prague)

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Rootstocks play a vital role in regulating the environmental adaptability and controlling the growth and development of apple trees. M9T337, an excellent apple rootstock widely used in commercial orchards, could confer dwarf tree architectures, early fruiting and suitability for high-density planting. However, the rooting ability of M9T3337 is low when it is vegetatively propagated, and researchers have not yet established an efficient micropropagation system. The present study systematically evaluated the multiplication in adventitious shoots and the in vitro formation of adventitious roots to determine the effects of the culture media and plant growth regulators of M9T337 and a rapid micropropagation system was developed. For the shoot multiplication, the highest multiplication index of 3.93 was obtained on Murashige and Skoog (MS) media supplemented with 2.0 mg/L 6-BA, 0.1 mg/L NAA and 0.3 mg/L GA3 from 12 combinations of 6-BA and NAA. Stronger and taller adventitious shoots were grown on MS supplemented with 1.8 mg/L 6-BA and 0.5 mg/L NAA. The optimal media with 100% rooting was obtained using ½ MS supplemented with 0.3 mg/L IBA or MS supplemented with 0.6 mg/L IBA for the rooting induction, resulting in mean rooting numbers of 13.00 and 11.33, respectively. Additionally, the effect on rooting of adding 0.3 mg/L IBA or not on the 1/2 MS and MS media was compared; the results suggested that an appropriate IBA concentration was the key to successful rooting. The rooted plantlets were acclimatised in a shaded greenhouse with an 84% survival rate. The established micropropagation system could be used for the rapid propagation of M9T337 for commercial production.

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An Efficient Micropropagation System for the Vulnerable Wild Apple Species, Malus sieversii, and Confirmation of Its Genetic Homogeneity

et al. 2022

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Development of a micropropagation protocol for Malus orientalis using axillary buds

Cited by 8 publications

References 36 publications

In vitro propagation and long-term observation of acclimated plants in endangered tree fern Alsophila costularis

In vitro propagation and long-term observation of acclimated plants in endangered tree fern Alsophila costularis

Establishment of an efficient micropropagation system in enhancing rooting efficiency via stem cuttings of apple rootstock M9T337

An Efficient Micropropagation System for the Vulnerable Wild Apple Species, Malus sieversii, and Confirmation of Its Genetic Homogeneity

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