Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair

Miyaguchi, Hajime; Kakuta, Masaya; Iwata, Yuko; Matsuda, Hideaki; Tazawa, Hidekatsu; Kimura, Hiroko; Inoue, Hiroyuki

doi:10.1016/j.chroma.2007.06.017

Cited by 58 publications

(57 citation statements)

References 18 publications

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“…66,67 Scissors used to cut hair should be cleaned between samples using a water-based detergent and/or wiped clean with a solvent (e.g. methanol).…”

Section: Sample Homogeneitymentioning

confidence: 99%

“…The majority of published hair testing methods are for drugs of abuse, including opiates, 42,60,69 cocaine, 64,87,98 amphetamines 66,67,75,84 and cannabinoids, 58,85,86,99 employing GC-MS or LC-MS-MS. Although new methods have been reported using specialist analytical instrumentation including quadruple time-of-flight and matrix-assisted laser desorption/ionization time of flight, 97,100 standard instrumentation and method parameters (temperature programme, ions selected for monitoring, collision energies, etc.)…”

Section: Confirmation Techniquesmentioning

confidence: 99%

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Hair testing is taking root

Cooper

2011

Ann Clin Biochem

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An increasing number of toxicology laboratories are choosing to expand the services they offer to include hair testing in response to customer demands. Hair provides the toxicologist with many advantages over conventional matrices in that it is easy to collect, is a robust and stable matrix that does not require refrigeration, and most importantly, provides a historical profile of an individual's exposure to drugs or analytes of interest. The establishment of hair as a complementary technique in forensic toxicology is a direct result of the success of the matrix in medicolegal cases and the wide range of applications. However, before introducing hair testing, laboratories must consider what additional requirements they will need that extend beyond simply adapting methodologies already validated for blood or urine. Hair presents many challenges with respect to the lack of available quality control materials, extensive sample handling protocols and low drug concentrations requiring greater instrument sensitivity. Unfortunately, a common pitfall involves over-interpretation of the findings and must be avoided.

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“…66,67 Scissors used to cut hair should be cleaned between samples using a water-based detergent and/or wiped clean with a solvent (e.g. methanol).…”

Section: Sample Homogeneitymentioning

confidence: 99%

Section: Confirmation Techniquesmentioning

confidence: 99%

Hair testing is taking root

Cooper

2011

Ann Clin Biochem

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“…Several common washing solvents have been reported, including water, methanol and acetone. The most common wash procedure is using 0.1% SDS and water to wash the hair alternately 3-5 times each (Takayama et al, 2003;Miyaguchi et al, 2007). To remove external contamination of hair, the wash procedure is necessary.…”

Section: Rat Hair Pretreatmentmentioning

confidence: 99%

The potential use of diisononyl phthalate metabolites hair as biomarkers to assess long-term exposure demonstrated by a rat model

Hsu

Liao

2015

Chemosphere

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“…The extraction of ketamine and its metabolites from hair samples is essential since hair is not a biological fluid but a solid sample. Therefore, the conventional acidic methanol extraction method was initially adopted for the rat hair (Srogi, 2006;Kintz, 2007;Musshoff and Madea, 2007a,b;Miyaguchi et al, 2007). As a consequence, no notable difference was observed in the extracted amounts of ketamine and norketamine when using MeOH-TFA = 50:1 (v/v), MeOH:5 M HCl = 20:1 (v/v), MeOH-5 M HCl = 9:1 (v/v), and 1 M HCl as the extraction solvents.…”

Section: Analysis Of Ketamine and Norketamine In Rat Hair Using Acidimentioning

confidence: 99%

“…For example, conventional acidic methanol extraction has been confirmed to be effective in the detection of ketamine and norketamine in hair; however, this method requires a long time for extraction. Recently, Miyaguchi et al (2007) have developed a micropulverized extraction method and succeeded in the rapid quantification of methamphetamine and amphetamine in human hair. This method is useful for the pretreatment of hair because it is rapid and requires a very small amount of hair.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of ketamine and norketamine in rat hair using micropulverized extraction and ultra‐performance liquid chromatography–electrospray ionization mass spectrometry

Inagaki

Makino

Fukushima

et al. 2009

Biomedical Chromatography

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A new method for the rapid and simultaneous detection of ketamine and its major metabolite, norketamine, in rat hair has been developed by combining micropulverized extraction and ultraperformance liquid chromatography-electrospray ionization mass spectrometry. By using reversed-phase UPLC, ketamine and norketamine were well separated within 2 min. Using ketamine-dosed rat hair, the conditions for micropulverized extraction were optimized, and the limits of detection and quantification of the developed method were found to be 1.7 and 5.7 pg/mg hair for ketamine, respectively. The precisions achieved with this method were slightly better than that obtained with conventional acidic methanol extraction method. Using this proposed method, analysis of the washed rat hair could be completed within 16-17 min. This method is expected to be applied for the analysis of the hair samples of not only rats but also ketamine abusers.

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Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair

Cited by 58 publications

References 18 publications

Hair testing is taking root

Hair testing is taking root

The potential use of diisononyl phthalate metabolites hair as biomarkers to assess long-term exposure demonstrated by a rat model

Rapid detection of ketamine and norketamine in rat hair using micropulverized extraction and ultra‐performance liquid chromatography–electrospray ionization mass spectrometry

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