Development of a mini core collection from Sri Lankan traditional rice for flowering time variation

Rathnathunga, E. U. U.; Senanayake, Gamini; Dissanayake, N.; Seneweera, Saman; Geekiyanage, Sudarshanee

doi:10.21475/ajcs.2016.10.09.p7865

Cited by 5 publications

(6 citation statements)

References 18 publications

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“…There are around 500 varieties in the collection while most of the varieties contain more than one accession. Our research group had characterized 755 accessions belonging to several varieties to be used as a breeding resource for manipulation of photoperiod responsiveness, nutrient quality and abiotic stress tolerance in the future: Using 384 accessions belonging to 55 varieties from above, a mini-core collection was developed for flowering time variation (Rathnathunga et al, 2016b). Among the several Sri Lankan rice varieties incorporated in breeding programmes at IRRI, submergence tolerance had been achieved through sub1A-2 gene of Sri Lankan rice James wee (Singh et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Within variety flowering time variation leads to yield variation in Sri Lankan traditional rice “Sudu wee”

Rathnathunga

Geekiyanage

2016

Ceylon J. Sci.

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Sri Lankan traditional rice is an important breeding resource for development of rice for ecological adaptation during the era of climate change. There are around 2000 accessions belonging to more than 500 varieties comprised of one to several accessions in each with wider morphological differences, flowering times and yield variations. Genetic identity of Sri Lankan traditional rice accessions belonging to each rice variety is not established at molecular level. Variety Sudu wee comprises of around 30 accessions at PGRC. Sudu wee could be useful in re-introduction programmes of traditional rice due to relatively shorter crop duration. The objective of the present study was to evaluate the diversity of 29 Sudu wee accessions from PGRC, based on days to flowering, (DF), 12 morphological and 2 physiological characters during non-inductive photoperiod season. DF varied from 62 to 106 days while accession 4193 did not flower during the experimental period of nearly 7 months. Leaf temperature at heading (LeT) and Leaf temperature difference at heading (LeTD), vegetative morphological characters and yield components varied among Sudu wee accessions. Seventy nine percent of total observed variation among accessions was explained through Principal Component Analysis. In the dendogram of accessions, 10 clusters were formed at the rescale distance of 5. Seed morphology indicated the genetic similarity among accessions while significantly different DF could be an indication of flowering time gene variation among similar genotypes. Increased DF reduced the grain yield increasing the vegetative growth among accessions of Sudu wee. Our results would be useful in future breeding for manipulating flowering time and yield within similar genetic backgrounds.

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Section: Introductionmentioning

confidence: 99%

Within variety flowering time variation leads to yield variation in Sri Lankan traditional rice “Sudu wee”

Rathnathunga

Geekiyanage

2016

Ceylon J. Sci.

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“…The present study processes the utmost significance as it is the first research project to employ DNA based varietal identification for rice in Sri Lanka. There are more than 2,000 rice accessions including landraces, wild types, and improved cultivars present in the country (Rathnathunga et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Establishment of a Scheme of DNA Fingerprinting and Sequencing to Distinguish the Caryopsis Samples of the Exportable Rice Cultivars from a Panel of Mega-Varieties

Ananda

Wijesundara

Herath

et al. 2020

SL J. Agric. Ecosys.

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The Rice Research and Development Institute (RRDI) of Sri Lanka has developed five exportable rice cultivars; At 362, Bg 94-1, Bg 360, Bg 1165-2, and Bw-Bs-1-2-31.The present study was conducted to establish the identities of these five cultivars at caryopsis level in comparison compared to those of mega rice cultivars (Bg 352, Bg 300, Bg 358, Bg 359, Bg 357, Bg 379-2, and At 353) in Sri Lanka using DNA fingerprinting and sequencing. These rice cultivars were grown in a greenhouse and a field using breeder-seeds. The seeds were harvested and subjected to a morphometric analysis using decision tree algorithms based on the size and colour of seeds and caryopses. The algorithms estimated the percentage accuracy of detection based on morphometric analysis ranged from 3.13-84.38 %. Similar seed and caryopsis combinations were grouped and exposed them to a panel of human subjects to discriminate the samples in each combination and subjected the data to calculate Kappa (K) and inter-rater reliability (IRR) statistics. The K was always 0.00, and IRR was 27% implying the inability of accurate visual differentiation. In the DNA fingerprinting analysis, a set of six SSR markers (RM206, RM246, RM251, RM335, RM475, and RM23744) were selected that can establish the cultivar identity. In addition, the combined analysis of DNA sequencing of 12 cultivars with three selected loci, (Seq 7-8, HvSSR12-34 and RM23744) authenticated the varietal identities.

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“…Rathnathunga et al (2016) developed a mini core collection of Sri Lankan traditional rice based on wide naturally occurring flowering time variation. In this study, a wide variation in flowering time for photoperiod was detected among selected early maturing accessions: twelve accessions were identified as delayed flowering under inductive SD condition, while fifteen accessions were early flowering under SD condition.…”

Section: Discussionmentioning

confidence: 99%

“…Sri Lanka possesses a wide gene pool of traditional rice with high environmental adaptability through different morphological and physiological characters including plant height, days to flowering (DF), grain shape, pericarp colour and presence of awns (Team of NRC Research Project 12-129, 2014, 2015. Rathnathunga et al (2016) evaluated 384 rice accessions from 53 Sri Lankan traditional rice varieties for morphological and flowering time variation and found that 39 accessions did not flower during the experimental period of 210 days in late short day season. According to PGRC (1999), there were 352 accessions, which flowered at or below 75 days under tested conditions in Sri Lanka.…”

Section: Introductionmentioning

confidence: 99%

Photoperiod sensitivity of very early maturing Sri Lankan rice for flowering time and plant architecture

Padukkage¹,

Senanayake²,

Geekiyanage³

2017

Open Agriculture

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Unavailability of information on photoperiod sensitivity of traditional rice is a disadvantage in breeding rice for adaptation to changing climatic conditions and for optimum plant architecture. This experiment was conducted to address the above problem through determination of variation in days to flowering (DF) and morphological traits of twenty eight selected very early maturing Sri Lankan traditional rice accessions under three photoperiod conditions: short day (SD), day neutral (DN) and long day (LD). Although rice is considered to be a facultative short day plant, 12 accessions (2088, 2979, 4615, 3970, 4245, 2091, 3450, 3883, 4217, 6741, 3738 and 3677) flowered significantly late under SD over LD, while accession 4223 flowered significantly late under DN. Fifteen accessions (3943, 4042, 4734, 3693, 4513, 3845, 4390, 4144, 4220, 4223, 4237, 4387, Bg 300, At 308 and Bg 379-2) flowered significantly early under SD over LD. Four accessions (3457, 3884, 6305 and 4358) were non-responsive to photoperiod for flowering time. Plant height significantly increased only in accession 4217 under SD. Higher plant height under DN was obtained from At 308 and accession 3883. Photoperiod did not affect plant height in accession 4237 and Bg 379-2. LD only or both LD and DN conditions produced significantly higher plant height irrespective of photoperiod responsiveness for DF in rest 26 accessions. Variation in response to photoperiod for DF and plant architecture in very early maturing Sri Lankan rice indicates the potential diversity of genetic factors for photoperiod response for future use in rice breeding.

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Development of a mini core collection from Sri Lankan traditional rice for flowering time variation

Cited by 5 publications

References 18 publications

Within variety flowering time variation leads to yield variation in Sri Lankan traditional rice “Sudu wee”

Within variety flowering time variation leads to yield variation in Sri Lankan traditional rice “Sudu wee”

Establishment of a Scheme of DNA Fingerprinting and Sequencing to Distinguish the Caryopsis Samples of the Exportable Rice Cultivars from a Panel of Mega-Varieties

Photoperiod sensitivity of very early maturing Sri Lankan rice for flowering time and plant architecture

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