Development of a Monoclonal Antibody Against Porcine Circovirus2 Cap Protein

Abstract

Female BALB/c mice were immunized with a commercial PCV2 vaccine, and a monoclonal antibody (MAb) designated as 3H11 was achieved by hybridoma techniques. The MAb specifically reacted with Cap protein of PCV2, which has been identified by western blot. Immunoperoxidase monolayer assay results showed that 3H11 did not cross-react with PCV1-infected cells. Therefore, this work suggested that 3H11 could be a useful tool as a specific diagnostic reagent for PCV2 research.

Structural roles of PCV2 capsid protein N-terminus in PCV2 particle assembly and identification of PCV2 type-specific neutralizing epitope

“…Hence, we assume that loop replacement will only change the local structures at the epitope regions but not the overall β-strands core region and capsid viral assembly. To this end, we performed systematic affinity screening between a PCV2 type-specific neutralizing mAb, named 3H11 [40], and chimeric PCV2 capsid by replacing the capsid protein surface loop sequence by corresponding PCV1 capsid protein sequence. We have made seven chimeric PCV2 capsid proteins by replacing the loop sequences at the following loop regions one by one: BC-loop (residues 58–66), CD-loop (residues 79–94), DE-loop (residues 108–116), EF-loop (residues 124–146), FG-loop (residues 153–156), GH-loop (residues 162–193) and HI-loop (residues 204–208) (S1 Table) .…”