Development of a Mouse Model for Chronic Cat Allergen-Induced Asthma

Grundström, Jeanette; Saarne, Tiiu; Kemi, Cecilia; Gregory, Joshua A.; Wadén, Konrad; Pils, Marina C.; Adner, Mikael; Gafvelin, Guro; Hage, Marianne van

doi:10.1159/000369066

Cited by 11 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our data indicated that low fat milk increased effectively both cell subsets in BALF comparably to OVA induced allergic airway inflammation. Consistent with the previous reports [5], [30], the pulmonary histological examination showed that OVA treated mice had various degrees of inflammation. Surprisingly, the milk challenged mice presented with more serious pulmonary inflammation as evident by increased eosinophil influx in lung and mucus hypersecretion.…”

Section: Discussionsupporting

confidence: 92%

Th2 related markers in milk allergic inflammatory mice model, versus OVA

El-housseiny

Ibrahim

Sellinger³

2017

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Experimental studies on allergic asthma are limited by the high cost of the administrated allergens. In this study we tested the allergic potency of low fat milk as a cheap substitute to the widely used standard allergen, ovalbumin (OVA). BALB/c female mice (4 weeks old) were sensitized intraperitoneally with low fat milk/or OVA followed by intranasal challenge with the two allergens on days 28 and 29. At day 31, serum, bronchoalveolar lavage fluid (BALF), and lungs were harvested. Mice of the low fat milk model showed infiltration of eosinophils, macrophages, lymphocytes, and neutrophils in BALF comparable to that of the OVA model. Both allergic protocols led to the production of similar numbers of Th2 cells and induced comparable expression of Th2 cytokine (IL-13) as evident by real time PCR for IL-13 and GATA3 (Th2 transcription factor) and confirmed by immunofluorescence for Th2 surface markers (T1/ST2). In addition, both mouse models had similar elevated levels of allergen specific antibody, IgG1 and IgE. Notably, HE, PAS, and LUNA stained lung sections from low fat milk treated mice had higher average pathological scores as compared to OVA treated mice. In conclusion, this study suggests that the low fat milk-induced inflammation showed hallmarks of allergic airway inflammatory model such as eosinophilic influx in BALF, increased numbers of Th2 cells, augmented expression of IL-13, elevated levels of circulatory IgG1 and IgE, signs of robust pulmonary inflammation, and most importantly it is a cheap and promising model for studying acute allergic airway inflammation and acute asthma.

show abstract

Section: Discussionsupporting

confidence: 92%

Th2 related markers in milk allergic inflammatory mice model, versus OVA

El-housseiny

Ibrahim

Sellinger³

2017

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…Lung sections stained by PAS were observed for numbers of goblet cells (stained red) in bronchiolar epithelium. A scoring system of 0–3 was used to indicate the relative number of goblet cells, with scores of 0 and 3 reflecting the same variations as described for H & E staining 38 , 39 . Lung sections stained by TRI were studied for collagen deposition (stained blue) and lung fibrosis, with scores of 0 and 3 reflecting the same variations as described for the two aforementioned staining methods.…”

Section: Methodsmentioning

confidence: 99%

Tregitope-linked Refined Allergen Vaccines for Immunotherapy in Cockroach Allergy

Prangtaworn

Chaisri

Seesuay

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Allergen-specific immunotherapy (AIT) facilitates long-term resolution of allergic morbidity resulting in reduced drug use and increased refractoriness to new sensitization. AIT effectiveness has been demonstrated in seasonal and perennial allergies, and insect stings. However, data and studies in AIT relative to cockroach (CR) allergy are relatively scarce. In this study, mice allergic to American CR (Periplaneta americana) were treated with a liposome (L)-entrapped vaccine made of mouse Tregitope289-Per a 9 of the CR, Tregitope167-Per a 9, or Per a 9 alone – or placebo. Allergic mice that received an individual vaccine intranasally had reduced Th2 response, reduced lung inflammation, and reduced respiratory tissue remodeling. However, only L-Tregitope289-Per a 9 and L-Tregitope167-Per a 9 induced expression of immunosuppressive cytokine genes (IL-10, TGF-β, and IL-35 for L-Tregitope289-Per a 9, and IL-10 and TGF-β for L-Tregitope167-Per a 9) and increment of idoleamine-2,3-dioxygenase 1 (IDO1), indicating that these vaccines caused allergic disease suppression and reversal of respiratory tissue remodeling via generation of regulatory lymphocytes. Liposome entrapped-recombinant Per a 9 (L-Per a 9) did not cause upregulation of immunosuppressive cytokine genes and IDO1 increment; rather, L-Per a 9 induced high expression of IFN-γ in lungs of treated mice, which resulted in mitigation of allergic manifestations. This study provides compelling evidence that both liposome-entrapped vaccines made of single refined major allergen alone and single refined major allergen linked with Tregitopes are effective for reducing allergen-mediated respiratory tissue inflammation and remodeling, but through different mechanisms.

show abstract

“…Sensitization and Challenge Protocols. Guinea pigs were sensitized twice through intranasal instillation of 200 μg of either HDM (Greer, Lenoir, NC, USA) or CDE (Greer, Lenoir, NC, USA), prepared as described previously [12], in 300 μL of PBS (Figure 1). On the challenge days, the guinea pigs were exposed to 100 μg of either HDM or CDE twice per week for 4, 8, or 12 weeks (Figure 1) under light anaesthesia with 5% isoflurane.…”

Section: Methodsmentioning

confidence: 99%

House Dust Mite and Cat Dander Extract Induce Asthma-Like Histopathology with an Increase of Mucosal Mast Cells in a Guinea Pig Model

Ramos‐Ramírez

Liu

Mogren

et al. 2023

Journal of Immunology Research

Self Cite

View full text Add to dashboard Cite

Background. Asthma is a chronic inflammatory disease with structural changes in the lungs defined as airway remodelling. Mast cell responses are important in asthma as they, upon activation, release mediators inducing bronchoconstriction, inflammatory cell recruitment, and often remodelling of the airways. As guinea pigs exhibit anatomical, physiological, and pharmacological features resembling human airways, including mast cell distribution and mediator release, we evaluated the effect of extracts from two common allergens, house dust mite (HDM) and cat dander (CDE), on histopathological changes and the composition of tryptase- and chymase-positive mast cells in the guinea pig lungs. Methods. Guinea pigs were exposed intranasally to HDM or CDE for 4, 8, and 12 weeks, and airway histology was examined at each time point. Hematoxylin and eosin, Picro-Sirius Red, and Periodic Acid-Schiff staining were performed to evaluate airway inflammation, collagen deposition, and mucus-producing cells. In addition, Astra blue and immunostaining against tryptase and chymase were used to visualize mast cells. Results. Repetitive administration of HDM or CDE led to the accumulation of inflammatory cells into the proximal and distal airways as well as increased airway smooth muscle mass. HDM exposure caused subepithelial collagen deposition and mucus cell hyperplasia at all three time points, whereas CDE exposure only caused these effects at 8 and 12 weeks. Both HDM and CDE induced a substantial increase in mast cells after 8 and 12 weeks of challenges. This increase was primarily due to mast cells expressing tryptase, but not chymase, thus indicating mucosal mast cells. Conclusions. We here show that exposure to HDM and CDE elicits asthma-like histopathology in guinea pigs with infiltration of inflammatory cells, airway remodelling, and accumulation of primarily mucosal mast cells. The results together encourage the use of HDM and CDE allergens for the stimulation of a clinically relevant asthma model in guinea pigs.

show abstract

Development of a Mouse Model for Chronic Cat Allergen-Induced Asthma

Cited by 11 publications

References 41 publications

Th2 related markers in milk allergic inflammatory mice model, versus OVA

Th2 related markers in milk allergic inflammatory mice model, versus OVA

Tregitope-linked Refined Allergen Vaccines for Immunotherapy in Cockroach Allergy

House Dust Mite and Cat Dander Extract Induce Asthma-Like Histopathology with an Increase of Mucosal Mast Cells in a Guinea Pig Model

Contact Info

Product

Resources

About