Development of a multicomponent immunochromatographic test system for the detection of fluoroquinolone and amphenicol antibiotics in dairy products

Abstract

BACKGROUND Ciprofloxacin (CIP) and chloramphenicol (CAP) are relevant antibiotics of the fluoroquinolone (FQ) and amphenicol (AP) groups, respectively, widely used in veterinary practice and they contaminate agricultural products. In this study, a rapid and sensitive immunochromatographic assay (ICA) was developed for simultaneous detection of CIP and CAP in dairy products. The ICA was carried out in a direct competitive format using gold nanoparticles as a label. RESULTS The ICA developed here allowed for the detection of CIP and CAP in Triton X‐100‐containing buffered saline (PBST) within 15 min with instrumental detection limits of 20 pg mL−1 and 0.5 ng mL−1, respectively, and with a visual detection limit of 5 ng mL−1 for both antibiotics. The ICA showed cross‐reactivity (69–160%) to 19 antibiotics in the FQ group and no cross‐reactivity (<0.1%) to 2 antibiotics of the AP group. The ICA allowed detection of CIP and CAP in a panel of dairy products by employing a simple procedure of preliminary sample preparation. The detection limits for the two antibiotics were the same as in PBST. The analytical recoveries of CIP and CAP in dairy products ranged from 83% to 120%. CONCLUSION The analytical characteristics of the test system allow its use for the detection of antibiotics in milk and dairy products during all steps of production. © 2019 Society of Chemical Industry

Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice

“…In the positive sample, the GNP-labeled mAb bound to DAS was not captured by the coating antigen, and as a result, the T line was light red or colorless. 28 Both free and bound GNP-labeled mAbs were captured by the goat anti-mouse IgG antibody on the C line, forming a deep red color. 29 Negative and positive samples were judged by comparison between the T line and the C line, and the results could be visually observed within 5 min.…”

A rapid colloidal gold‐based immunochromatographic strip assay for monitoring nitroxynil in milk

“…Therefore it is essential to develop a rapid, simple and effective device for NIT detection in the field. Competitive format immunochromatographic strip assay based on the specific binding of antibody and antigen possesses distinct benefits for rapidly screening small analytes . Its user‐friendly format, simple analysis and low cost make it commonly used in real‐time detection of veterinary drug residues .…”

“…Competitive format immunochromatographic strip assay based on the specific binding of antibody and antigen possesses distinct benefits for rapidly screening small analytes. [19][20][21][22][23] Its user-friendly format, simple analysis and low cost make it commonly used in real-time detection of veterinary drug residues. [24][25][26][27][28] To the best of our knowledge, only one paper has reported an application for NIT detection based on the immunological method.…”

A highly sensitive monoclonal antibody−based paper sensor for simultaneously detecting valnemulin and tiamulin in porcine liver

“…Particularly, ICA results can be obtained and visually interpreted within 10 min. Up to now, ICA has been extensively applied in the rapid identification of toxins (Lan et al., 2019; Qiu et al., 2019), antibiotics (Hendrickson, Zvereva, Shanin, Zherdev, & Dzantiev, 2019; Yang et al., 2019), food additives (Deng et al., 2019), and other chemicals (Guo et al., 2019; Zvereva, Zherdev, Xu, & Dzantiev, 2018) in food components. As far as the authors know, a few literatures about the immunological methods for detecting PLEs have been reported.…”