Development of a multiplex Luminex assay for detecting swine antibodies to structural and nonstructural proteins of foot-and-mouth disease virus in Taiwan

Chen, Tsuhan; Lee, Fan; Lin, Yu‐Li; Pan, Chu-Hsiang; Shih, Chia-Ni; Tseng, Chih‐Wei; Tsai, Hsiang‐Jung

doi:10.1016/j.jmii.2014.05.009

Cited by 6 publications

(3 citation statements)

References 46 publications

(27 reference statements)

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“…The purified protein and antibodies were then shown to be specific to serotype O when tested against serotypes A, O, C and Asia-1 in an enzyme-linked immunosorbent assay (ELISA) and latex agglutination test (Suryanarayana et al 1999). The reliable differentiation of serotype-specific antibodies with recombinant VP1 was demonstrated by Wenger et al (1999) and by Chen et al (2016) in an indirect ELISA and Luminex assay respectively. Recombinant VP1 protein was also used as antigen on an immunochromatographic strip for specific rapid detection of FMDV serotype O (Yang et al 2010).…”

Section: Introductionmentioning

confidence: 98%

Production of foot-and-mouth disease virus SAT2 VP1 protein

et al. 2020

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The seven serotypes of foot-and-mouth disease virus (FMDV) differ on the surface exposed regions on the VP1, 2 and 3 proteins. Amongst the three, the VP1 protein has been produced the most for use in serotyping assays for some of the Euro-Asian serotypes. In this study the VP1 protein of the FMDV SAT2/ZIM/7/83 was expressed in Escherichia coli BL21 cells in Luria broth and EnPresso ® B media in shake flasks. Production was further developed and the VP1 protein was produced at 2.15 g L −1 in fed-batch fermentations at 2 L scale. The protein formed insoluble inclusion bodies that were isolated, denatured and refolded. When tested in ELISA, the protein was found to be highly reactive with serum from a SAT2 vaccinated guinea pig, and not reactive to SAT1 and SAT3 antisera. These results open avenues to evaluate recombinantly expressed VP1 proteins for differentiation of the three Southern African Territories serotypes of FMDV that co-occur in Southern and East Africa. In addition, this could mitigate the need for employing virus as reagent, or having to raise reagent antibodies.

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Section: Introductionmentioning

confidence: 98%

Production of foot-and-mouth disease virus SAT2 VP1 protein

et al. 2020

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“…However, less work has been carried out using this technology in the veterinary field (32–38) and there are only a few commercial kits available. Moreover, when compared to conventional ELISA, previous results have shown that xMAP formats can be more sensitive and reproducible (35).…”

Section: Introductionmentioning

confidence: 99%

Bead-Based Multiplex Assay for the Simultaneous Detection of Antibodies to African Swine Fever Virus and Classical Swine Fever Virus

et al. 2019

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African swine fever (ASF) and Classical swine fever (CSF) are both highly contagious diseases of domestic pigs and wild boar. In the last years, several cases of both diseases have been reported in the Caucasus, Russian Federation and Eastern Europe. Thus, the probability of encountering these two viruses in the same area is increasing. Since differentiation by clinical or post-mortem examination is not possible, laboratory tools for differential diagnosis are required. In the present work, we have developed a triplex bead-based assay using some of the most immunogenic antigens of each virus, for the simultaneous detection of antibodies; i.e. the VP72 and VP30 of ASF virus (ASFV) and the E2 protein of CSF virus (CSFV). The assay was firstly set up and optimized using well characterized reference serum samples specific for each pathogen. Then, a panel of 352 sera from experimentally infected animals with either ASFV or CSFV were analyzed in the multiplex assay. A collection of 253 field negative sera was also included in the study. The results of the multiplex analysis were compared to those obtained by two commercially available ELISAs for detection of antibodies against ASFV or CSFV, and considered in this study as the reference techniques. The data obtained showed values of 97.3% sensitivity and 98.3% specificity for detection of antibodies to ASFV and 95.7% of sensitivity and 99.8% specificity for detection of antibodies to CSFV. This multiplex assay allows the simultaneous and differential detection of antibodies against ASFV and CSFV, providing a valuable tool for surveillance studies. Moreover, this method is rather versatile, offering the possibility of increasing the panel of antigens from other swine diseases that could be of interest for a differential diagnosis along with ASF and CSF.

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“…This technology combines fluorescent-dyed microspheres, a detection instrument based on lasers read-out, digital signal processing and an analysis software (Christopher-Hennings et al, 2013;Graham et al, 2019). While BBMAs are widely applied in human health, with the development of methods for drug discovery (Komnatnyy et al, 2018), diseases diagnosis (Grignard et al, 2019;Lu et al, 2005) and immune response characterisation (Jones et al, 2002) among others, work in the veterinary field has been more limited, although interest has been rising recently (Chen et al, 2016;Fabian et al, 2020;Hoste et al, 2020;Laamiri et al, 2016;Ragan et al, 2018). To date, there are a few commercial assays available (References TRD-500 and TRD-502, Biovet Inc. Saint-Hyacinthe, Canada).…”

Section: Introductionmentioning

confidence: 99%

A multiplex assay for the detection of antibodies to relevant swine pathogens in serum

Aira

Penning

Eiden

et al. 2021

Transbounding Emerging Dis

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Livestock industry supports the livelihood of around 1.3 billion people in the world, with swine industry contributing with 30% of total livestock production worldwide. To maintain and guarantee this production, a pivotal point according to the OIE is addressing potential biohazards. To control them, permanent sero-surveillance is crucial to achieve more focused veterinary public health intervention and prevention strategies, to break the chains of transmission, and to enable fast responses against outbreaks.Within this context, multiplex assays are powerful tools with the potential to simplify surveillance programs, since they reduce time, labour, and variability within analysis.In the present work, we developed a multiplex bead-based assay for the detection of specific antibodies to six relevant pathogens affecting swine: ASFV, CSFV, PRRSV, SIV, TB and HEV. The most immunogenic target antigen of each pathogen was selected as the target protein to coat different microsphere regions in order to develop this multiplex assay. A total of 1544 serum samples from experimental infections as well as field samples were included in the analysis. The 6-plex assay exhibited credible diagnostic parameters with sensitivities ranging from 87.0% to 97.5% and specificities ranging from 87.9% to 100.0%, demonstrating it to be a potential high throughput tool for surveillance of infectious diseases in swine.

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Development of a multiplex Luminex assay for detecting swine antibodies to structural and nonstructural proteins of foot-and-mouth disease virus in Taiwan

Abstract: The results indicated that xMAP has the potential to detect antibodies to FMDV-SP-VP1 and NSP-3ABC and to distinguish FMDV-infected pigs from pigs infected with the swine vesicular disease virus.

Cited by 6 publications

References 46 publications

Production of foot-and-mouth disease virus SAT2 VP1 protein

Production of foot-and-mouth disease virus SAT2 VP1 protein

Bead-Based Multiplex Assay for the Simultaneous Detection of Antibodies to African Swine Fever Virus and Classical Swine Fever Virus

A multiplex assay for the detection of antibodies to relevant swine pathogens in serum

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