Development of a multiplex probe combination-based one-step real-time reverse transcription-PCR for NA subtype typing of avian influenza virus

Sun, Zhihao; Qin, Tao; Meng, Fanqiang; Chen, Sujuan; Peng, Daxin; Liu, Xiufan

doi:10.1038/s41598-017-13768-4

Cited by 8 publications

(8 citation statements)

References 31 publications

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“…Three In uenza A virus isolates A/mallard/Huadong/S/2005 (H5N1) [18], A/chicken/Jiangsu/WJ-14/2015 (H7N9) [19] and A/chicken/Taixing/10/2010 (H9N2) [20] were used in this study. Viruses were ampli ed in 10-day-old speci c-pathogen-free (SPF) chicken embryonated eggs.…”

Section: Methodsmentioning

confidence: 99%

Influenza A virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1

Yang

Wang

et al. 2020

Preprint

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BACKGROUND: Although influenza A virus (IAV) employs diverse strategies to evade IFN responses by inhibiting the synthesis of IFN, how IAV regulates signaling downstream of IFN is incompletely understood.METHODS: In this study, we used Western blot-based protein analysis coupled with RT-qPCR, overexpression and RNA interference to investigate the regulation of JAK1 by IAV infection.RESULTS: The results indicated that JAK1 was ubiquitinated and degraded, resulting in inhibition of type I and type II IFN responses, demonstrating that IAV antagonizes the IFN-activated JAK/STAT signaling pathway by inducing the degradation of JAK1. Furthermore. IAV infection upregulated the suppressor of cytokine signaling (SOCS) protein SOCS1, and SOCS1 mediated the ubiquitination and degradation of JAK1. CONCLUSION: Collectively, our findings suggest that IAV infection induces SOCS1 expression to promote JAK1 degradation, which in turn inhibits host innate immune responses.

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Section: Methodsmentioning

confidence: 99%

Influenza A virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1

Yang

Wang

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Three Influenza A virus isolates A/mallard/Huadong/ S/2005 (H5N1) [18], A/chicken/Jiangsu/WJ-14/2015 (H7N9) [19] and A/chicken/Taixing/10/2010 (H9N2) [20] were used in this study. Viruses were amplified in 10-day-old specific-pathogen-free (SPF) chicken embryonated eggs.…”

Section: Virus and Cellsmentioning

confidence: 99%

Influenza a virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1

Yang

Wang

et al. 2020

Virol J

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Background: Although influenza A virus (IAV) employs diverse strategies to evade IFN responses by inhibiting the synthesis of IFN, how IAV regulates signaling downstream of IFN is incompletely understood. Methods: In this study, we used Western blot-based protein analysis coupled with RT-qPCR, overexpression and RNA interference to investigate the regulation of JAK1 by IAV infection. Results: The results indicated that JAK1 was ubiquitinated and degraded, resulting in inhibition of type I and type II IFN responses, demonstrating that IAV antagonizes the IFN-activated JAK/STAT signaling pathway by inducing the degradation of JAK1. Furthermore. IAV infection upregulated the suppressor of cytokine signaling (SOCS) protein SOCS1, and SOCS1 mediated the ubiquitination and degradation of JAK1. Conclusion: Collectively, our findings suggest that IAV infection induces SOCS1 expression to promote JAK1 degradation, which in turn inhibits host innate immune responses.

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“…A/chicken/Jiangsu/WJ-14/2015 (H7N9) was obtained from the Key Laboratory for Animal Infectious Diseases, Ministry of Agriculture, Yangzhou University, Jiangsu, China (Sun et al, 2017). Strain was propagated in 10-day-old specificpathogen-free (SPF) embryonic chicken eggs.…”

Section: Viruses and Cellsmentioning

confidence: 99%

Evaluation of the Viricidal Efficacy of Four Chemical Disinfectants against Emerged H7N9 Avian Influenza Virus in China

Peng

Chen

Miao

et al. 2020

IJAR

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Background: Establishment of scientific disinfection measures in domestic poultry farms and live-poultry markets (LPMs) are critical to prevent the spread of H7N9 subtype avian influenza that outbreaks in poultry and infection in humans. The current study was aimed to evaluate the efficacy of four common disinfectants to inactivate H7N9 subtype avian influenza virus (AIV). Methods: According to the disinfection technical specification, the average inactivation logarithmic values of four disinfectants against the epidemic H7N9 subtype AIV, including deciquam solution, glutaral and benzalkonium bromide solution, sodium dichloroisocyanurate powder, and peroxyacetic acid solution, were systematically evaluated. Result: Our data showed that incubation of 0.005% (w/v) deciquam solution for 30 min contact time, or 0.05% (v/v) glutaral and benzalkonium bromide solution for 10 min, or 0.24 g/L sodium dichloroisocyanurate powder for 30 min, or 0.05% (v/v) peroxyacetic acid solution for 10 min, can completely inactivate H7N9 subtype AIV. These results suggested that glutaral and benzalkonium bromide solution as well as peroxyacetic acid solution at recommended concentrations can be effectively used to cut off the spread of H7N9 subtype AIV in poultry farms and LPMs, however, deciquam solution and sodium dichloroisocyanurate powder need to increase their working concentration.

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Development of a multiplex probe combination-based one-step real-time reverse transcription-PCR for NA subtype typing of avian influenza virus

Cited by 8 publications

References 31 publications

Influenza A virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1

Influenza A virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1

Influenza a virus antagonizes type I and type II interferon responses via SOCS1-dependent ubiquitination and degradation of JAK1

Evaluation of the Viricidal Efficacy of Four Chemical Disinfectants against Emerged H7N9 Avian Influenza Virus in China

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