Development of a Murine Model of Chronic Rhinosinusitis

Abstract

B-2.

From Mouse to Man: Translational Value of Animal Models of Allergic Rhinitis

“…Beyond this example, comparative descriptions of nasal mast cell histopathology are rarely reported in experimental AR models. In a more recent report in a chronic mouse model of fungal AR, descriptions of mast cell kinetics into the epithelium and mucosa are very similar to that found in human AR (Lindsay et al 2006). Despite the subjective evaluation and misidentification of nasal anatomy, this model reproduces many key features of human AR besides mast cell pathology.…”

“…However most of these studies have been based on inappropriate application of mouse anatomy to human disease. Specifically, the airspaces between the ethmoid turbinates of mice ( Figure 3B), have been misinterpreted to be analogous to human ethmoid sinuses (Bomer et al 1998;Jacob and Chole 2006;Lindsay et al 2006;Phillips et al 2009). Ethmoid turbinates in mice do not enclose sinus airways, are lined predominantly by olfactory neuroepithelium, and receive significant airflow Kimbell et al 1997).…”

Clara Cell 10-kD Protein Suppresses Chitinase 3-Like 1 Expression Associated with Eosinophilic Chronic Rhinosinusitis

“…Twenty-four hours after the last challenge (Day 103), nasal lavage fluid (NLF) was collected and the levels of interleukin IL-1b, tumor necrosis factor (TNF)-a, interferon (INF)-g, IL-5, IL-13, and eotaxin-1 in NLF were determined by ELISA as previously described (5). Sinonasal cavity structure was dissected, decalcified, embedded in paraffin, and sectioned as mentioned elsewhere (4,5). Histological examination was performed according to the methods previously reported (4,5).…”

“…Sinonasal cavity structure was dissected, decalcified, embedded in paraffin, and sectioned as mentioned elsewhere (4,5). Histological examination was performed according to the methods previously reported (4,5). In some experiments, respiratory sinonasal mucosa was dissected under a microscope and total RNA was extracted by using an RNeasy kit (Qiagen, Valencia, Calif).…”

“…Variable evidence has suggested that ostial patency impairment, mucociliary dysfunction, allergy, microorganism infection, and genetic factors are associated with CRS, but the etiology of CRS still remains largely unknown (1,2). Recently, based on previously published work (3,4), we developed a murine model of allergic ECRS (5), which mimics human ECRS well at the immunopathologic level and may allow us to study the regulatory mechanisms underlying the prolonged and exaggerated inflammatory processes in CRS (5,6).…”