2023
DOI: 10.3389/fbioe.2023.1225849
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Development of a native-locus dual reporter system for the efficient screening of the hyper-production of natural products in Streptomyces

Abstract: Streptomyces is renowned for its abundant production of bioactive secondary metabolites, but most of these natural products are produced in low yields. Traditional rational network refactoring is highly dependent on the comprehensive understanding of regulatory mechanisms and multiple manipulations of genome editing. Though random mutagenesis is fairly straightforward, it lacks a general and effective strategy for high throughput screening of the desired strains. Here in an antibiotic daptomycin producer S. ro… Show more

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“…On day 4, when the wild type strain (WT) begins to form the white aerial mycelia, both ΔlimB and limB -overexpression strains grow as substrate mycelia. Moreover, the red pigment, which is produced from a type II polyketide synthase 30 , 31 , is only produced in WT at this time point. On day 7, when the limB -overexpression strain has developed to aerial mycelia and produced the red pigment, no aerial mycelia is observed in the ΔlimB mutant yet.…”
Section: Resultsmentioning
confidence: 96%
“…On day 4, when the wild type strain (WT) begins to form the white aerial mycelia, both ΔlimB and limB -overexpression strains grow as substrate mycelia. Moreover, the red pigment, which is produced from a type II polyketide synthase 30 , 31 , is only produced in WT at this time point. On day 7, when the limB -overexpression strain has developed to aerial mycelia and produced the red pigment, no aerial mycelia is observed in the ΔlimB mutant yet.…”
Section: Resultsmentioning
confidence: 96%