Development of a new 7BS.7HL winter wheat-winter barley Robertsonian translocation line conferring increased salt tolerance and (1,3;1,4)-β-D-glucan content

Türkösi, Edina; Darkó, Éva; Rakszegi, Marianna; Molnár, István; Molnár-Láng, Márta; Cseh, András

doi:10.1371/journal.pone.0206248

Cited by 15 publications

(13 citation statements)

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“…The frequency of PMCs where the centromere of the 2S k univalent divided was 7.89%. Our results are comparable with other reports, showing that the frequency of univalent misdivision range between 3% up to 51% [26,30,31]. When both approaches were compared (40 + M2R + M2S k vs 40 + M2RS + M2RL + M2S k ), no significant difference in the frequency at which the Aegilops univalent misdivided was observed.…”

Section: Discussionsupporting

confidence: 90%

“…Many reports have shown that RobTs in wheat can arise by centromeric misdivision of univalents at AI, followed by segregation of the derived telocentric chromosomes to the same nucleus, and fusion of the broken parts [26,30,31]. The focus of this study was to exploit the formation of RobTs to transfer of the Lr54 + Yr37 gene loci from Ae.…”

Section: Discussionmentioning

confidence: 99%

“…Wheat breeders can use a large collection of aneuploid stocks for the induction of wheat-wheat and wheat-alien RobTs. This can be performed in a directed manner by producing the appropriate wheat or alien monosomic lines [28][29][30][31]. In wheat breeding the most common RobTs are the T1BL.1RS and T1AL.1RS translocations [25].…”

mentioning

confidence: 99%

“…Another issue is the resultant telocentric chromosomes have a tendency of rejoin as RobTs. It was reported that the frequency of wheat-alien RobTs recovered ranges from 4% to 20%, depending on the genetic background, the chromosomes involved, and environmental conditions used [26,30,31].To increase the rate of RobT formation, it is therefore necessary to reduce the random factors connected with appearance of centric breaks. In this study, we postulated that the use of telosomic plants for cross-hybridization with plants carrying an alien-substitution will overcome the random process of centric break formation in the univalent (Figure 1b).…”

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confidence: 99%

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confidence: 99%

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Recovery of 2R.2Sk Triticale-Aegilops kotschyi Robertsonian Chromosome Translocations

et al. 2019

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Robertsonian translocations (RobTs) in the progeny of triticale (×Triticosecale Wittmack) plants with monosomic substitution of Aegilops kotschyi chromosome 2S k (2R) were investigated by fluorescence in-situ hybridization. Chromosome 2S k of Ae. kotschyi is reported to possess many valuable loci, such as Lr54 + Yr37 leaf and stripe (yellow) rust resistance genes. We used a standard procedure to produce RobTs, which consisted of self-pollination of monosomic triticale plants, carrying 2R and 2S k chromosomes in monosomic condition. This approach did not result in RobTs. Simultaneously, we succeeded in producing 11 plants carrying 2R.2S k compensatory RobTs using an alternative approach that utilized ditelosomic lines of triticale carrying 2RS (short arm) and 2RL (long arm) telosomic chromosomes. Identification of molecular markers linked to Lr54 + Yr37 genes in the translocation plants confirmed that these resources can be exploited in current triticale breeding programmes.Agronomy 2019, 9, 646 2 of 12 from wild relatives into the wheat genetic background [10]. Recently, several attempts were made to transfer rust resistance genes from Aegilops, Agropyron and Triticum species into triticale [11][12][13][14][15].Aegilops species are closely related to wheat (and triticale, per se) and carry a number of valuable traits, which have been effectively incorporated into wheat by developing wheat-Aegilops hybrids and deriving addition, substitution and translocation lines [16]. Aegilops kotschyi Boiss. (2n = 4x = 28 chromosomes, U-and S-genomes) is a wild tetraploid goatgrass native to Northern Africa, the Mid-East, and Western Asia. Ae. kotschyi germplasm is exploited in wheat breeding [17] as a source of high grain protein, iron and zinc [18]. Moreover, Antonov and Marais [19] observed leaf rust resistance that was effective against the infection of Puccinia triticina in Ae. kotschyi. Marais et al. [20] identified the Lr54 and Yr37 leaf rust and stripe rust resistance genes, and developed aT2DS.2S k L wheat-Ae. kotschyi translocation line. The first Lr54 + Yr37 marker was developed by Heyns et al. [21]. Moreover, translocation gene sequences were cloned and specific SSR markers were developed [22].Homoeologous recombination based engineering is the most common way for efficiently utilizing the wild relative gene pool for crop improvement [23]. The generation of translocation lines is the most promising pathway for the exploitation of alien germplasm in crop breeding [23]. In distant hybrids, unpaired chromosomes are present as univalents during meiosis. Monosomic chromosomes are prone to centric breaks at anaphase I of meiosis, which misdivide and the broken ends fuse during the interkinesis of meiosis II [24][25][26]. Fusion of the misdivided products may result in the formation of a Robertsonian translocation (RobT) [27].Several steps are required to generate RobTs (Figure 1a), with self-pollination of double-monosomic plants being the most common method used in the induction of RobTs [26]. Wheat breeders can use a l...

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%