Development of a new high‐performance liquid chromatography‐mass spectrometric method for the analysis of ecdysteroids in biological extracts

Wainwright, Geoffrey; Prescott, Mark; Lomas, Lee; Webster, S. G.; Rees, Huw H.

doi:10.1002/(sici)1520-6327(1997)35:1/2<21::aid-arch3>3.0.co;2-#

Cited by 18 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wainwright et al . [25] reported that for free ecdysteroids, the major ions observed in the spectra are the protonated pseudomolecular ion [M+H] + and ions corresponding to the successive losses of water molecules, [M+H- n H 2 0] + ( n = 1–4).…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Analysis of Pfaffia glomerata Inflorescences by LC-ESI-MS/MS

et al. 2014

View full text Add to dashboard Cite

Pfaffia glomerata contains high levels of β-ecdysone, which has shown a range of beneficial pharmacological effects. The present study demonstrated that inflorescences of P. glomerata contain other important bioactive compounds in addition to β-ecdysone. The identification of compounds from inflorescences using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) was performed for the first time. The eight compounds identified were β-ecdysone, flavonoid glycosides such as quercetin-3-O-glucoside, kaempferol-3-O-glucoside and kaempferol-3-O-(6-pcoumaroyl)-glucoside, oleanane-type triterpenoid saponins such as ginsenoside Ro and chikusetsusaponin IV, in addition to oleanonic acid and gluconic acid. This study provided information on the phytochemicals contained in P. glomerata inflorescences revealing the potential application of this plant part as raw material for the phytotherapeutic and cosmetic industries.

show abstract

Section: Resultsmentioning

confidence: 99%

Phytochemical Analysis of Pfaffia glomerata Inflorescences by LC-ESI-MS/MS

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The final methanol extract was concentrated to 50 µl, and 10 µl was injected onto an HPLC C16 column (Diaspher-C16, BioChemMak ST, Moscow, Russia, 2×150 mm, pore size 110A, particle size 5 µm), the mobile phase was 15% acetonitrile in water and the flow rate was 0.5 ml/ min. 20-hydroxyecdysone and 25-S-inokosterone concentrations were determined by HPLC with a quadrupole mass-spectrometric detection system (Agilent 1100 Series LC/MSD VL, Palo Alto, CA) in the SIM mode according to Wainwright et al (1997). Sample size varied from 5 to 6 measurements for each group.…”

Section: E Content Measurementsmentioning

confidence: 99%

Effects of octopamine on reproduction, juvenile hormone metabolism, dopamine, and 20‐hydroxyecdysone contents in Drosophila

Грунтенко

Karpova

Alekseev

et al. 2007

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The effect of an experimentally increased octopamine content (feeding flies with OA) on the levels of juvenile hormone (JH) degradation, dopamine (DA), and 20-hydroxyecdysone (20E) contents, oogenesis, and fecundity of wild type Drosophila flies has been studied. OA feeding of the flies was found to (1) cause a considerable decrease in JH degradation in females, but not males, of D. melanogaster and D. virilis; (2) have no effect on DA content in D. melanogaster and D. virilis; (3) increase 20E contents in D. virilis females; (4) decrease to a large extent the number of vitellogenic (stages 8-10) and mature (stage 14) oocytes in D. virilis; and (5) decrease the fecundity of D. melanogaster and D. virilis. A possible mechanism of action of OA as a neurohormone on the reproductive function of Drosophila is discussed.

show abstract

“…Ecdysteroids from haemolymph and tissue samples were extracted and analysed by methods modified from Lafont et al (1982) and Wainwright et al (1997). Haemolymph (approximately 0.5 ml) and tissue samples (50 to 200 mg wet weight) were mixed with 8 ml water and 8 ml chloroform.…”

Section: Ecdysteroid Measurementsmentioning

confidence: 99%

Variations in ecdysteroid levels and Cytochrome p450 expression during moult and reproduction in male shore crabsCarcinus maenas

Styrishave

Rewitz²,

Lund³

et al. 2004

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Ecdysteroid levels were investigated by HPLC-MS over the moult cycle and in relation to reproduction in male shore crabs Carcinus maenas. Ecdysone (E), 20-hydroxyecdysone (20E) and Ponasterone A (PoA) were quantified in the haemolymph, hepatopancreas and testis. Also, the expression of 2 recently discovered Cytochrome p 450 genes (CYP330A1 and CYP4C39) inducible by ecdysteroids was studied in the hepatopancreas by Northern blot hybridisation analysis. In the haemolymph and hepatopancreas, all 3 ecdysteroids varied over the moult cycle with high levels in premoult and low levels in postmoult and intermoult. In the testis, 20E and E were present at high levels except in Postmoult Stage A, where low levels were observed. PoA was never observed in the testis. Ecdysteroids were quantified in the red and green colour forms of late intermoult C 4 crabs. In both phenotypes, 20E was the dominating ecdysteroid in late intermoult. In the haemolymph, 20E levels did not vary between the 2 phenotypes, but haemolymph 20E levels were negatively related to size. Also, haemolymph 20E levels varied with season in late-intermoult crabs, with higher levels during spring and autumn than during summer. Green crabs had significantly higher testicular E levels than red crabs. Ecdysteroid levels were negatively related to CYP330A1 and CYP4C39 gene expression. CYP330A1 and CYP4C39 mRNA levels were low during intermoult and premoult but high during postmoult. The results suggest that E and 20E are involved in both growth and reproduction whereas PoA is involved in moulting but not in reproduction and that the testis of male shore crab may be a possible source of ecdysone production in addition to the Y-organ. The results also support the concept that the probability of male shore crabs entering a new moult cycle decreases with increasing size.

show abstract

Development of a new high‐performance liquid chromatography‐mass spectrometric method for the analysis of ecdysteroids in biological extracts

Cited by 18 publications

References 16 publications

Phytochemical Analysis of Pfaffia glomerata Inflorescences by LC-ESI-MS/MS

Phytochemical Analysis of Pfaffia glomerata Inflorescences by LC-ESI-MS/MS

Effects of octopamine on reproduction, juvenile hormone metabolism, dopamine, and 20‐hydroxyecdysone contents in Drosophila

Variations in ecdysteroid levels and Cytochrome p450 expression during moult and reproduction in male shore crabsCarcinus maenas

Contact Info

Product

Resources

About