Development of a novel aptasensor using jellyfish collagen as matrix and thrombin detection in blood samples obtained from patients with various neurodisease

Derkuş, Burak; Arslan, Yavuz Emre; Bayraç, Abdullah Tahir; Kantarcıoğlu, İlkim; Emregül, Kaan C.; Emregül, Emel

doi:10.1016/j.snb.2016.01.095

Cited by 42 publications

(29 citation statements)

References 54 publications

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“…The jellyfish collagen, isolated and characterized by our group as previously described [28], was prepared by dissolving in 0.1 M acetic acid so as to obtain the desired concentration (1 mg.mL -1 ) in the final solution. To prepare the jellyfish collagen-nHA-TBA electrodes, HA nanoparticles were added (considering the HA:Collagen ratio of 1:10 w/w) into the eppendorf in which the jellyfish collagen (0.42 mg.mL -1 ) was situated.…”

Section: Preparation Of Jellyfish Collagen-nha-tba Aptasensormentioning

confidence: 99%

Enhancement of aptamer immobilization using egg shell-derived nano-sized spherical hydroxyapatite for thrombin detection in neuroclinic

Derkuş

Arslan

Emregül

et al. 2016

Talanta

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Section: Preparation Of Jellyfish Collagen-nha-tba Aptasensormentioning

confidence: 99%

Enhancement of aptamer immobilization using egg shell-derived nano-sized spherical hydroxyapatite for thrombin detection in neuroclinic

Derkuş

Arslan

Emregül

et al. 2016

Talanta

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“…[4][5][6] These in vivo applications include wound dressings, 7 artificial tissue and organ production, 8 cartilage tissue regeneration, 9,10 drug delivery systems, 11 and biomedical engineering. 12 Collagen has been extracted from a range of sources, but is commonly obtained from bovine, porcine, and equine sources for in vivo use. 13 However, these sources have problems with bovine spongiform encephalopathy (BSE), other transmittable spongiform encephalopathies (TSEs), and potential viral vectors that could be transmissible to humans.…”

Section: Introductionmentioning

confidence: 99%

In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices

Widdowson

Picton

Vince³

et al. 2017

J Biomed Mater Res

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Jellyfish have emerged as a source of next generation collagen that is an attractive alternative to existing sources, such as bovine and porcine, due to a plentiful supply and providing a safer source through lack of bovine spongiform encephalopathy (BSE) transmission risk and potential viral vectors, both of which could be transmitted to humans. Here we compare collagen implantable sponges derived for the first time from the Rhizostoma pulmo jellyfish. A further novelty for the research was that there was a comparison for sponges that were either uncrosslinked or crosslinked using 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDC), and an assessment on how this affected resorption, as well as their biocompatibility compared to bovine type I collagen sponges. The scaffolds were prepared and examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and scanning electron microscopy (SEM). The samples were implanted in adult male Wistar rats for in vivo experimentation. Both crosslinked and uncrosslinked jellyfish collagen sponges showed a significant reduction in histopathology scores over the course of the study, whereas the bovine collagen sponge scores were not significantly reduced. Both jellyfish collagen sponges and the bovine sponge were tolerated well by the hosts, and a recovery was visible in all samples, suggesting that R. pulmo jellyfish‐derived collagen could offer compelling biocompatibility with wound healing applications. We also demonstrate that noncrosslinked samples could be safer with better resorption times than crosslinked samples. © 2017 The Authors Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1524–1533, 2018.

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“…Protein extracts were centrifuged at 12000 rpm for 1 hr, and collected supernatants were stored at 4°C until use. Finally, the protein composition was analyzed using SDS‐PAGE technique as previously described by our group . Protein extracts obtained from BSC tissue and 3D‐dCBS were subjected to electrophoresis in 12% polyacrylamide gel.…”

Section: Methodsmentioning

confidence: 99%

A novel method for constructing an acellular 3D biomatrix from bovine spinal cord for neural tissue engineering applications

Arslan

Efe

Arslan

2019

Biotechnology Progress

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In this study, we aimed at generating 3‐dimensional (3D) decellularized bovine spinal cord extracellular matrix‐based scaffolds (3D‐dCBS) for neural tissue engineering applications. Within this scope, bovine spinal cord tissue pieces were homogenized in 0.1 M NaOH and this viscous mixture was molded to attain 3D bioscaffolds. After resultant bioscaffolds were chemically crosslinked, the decellularization process was conducted with detergent, buffer, and enzyme solutions. Nuclear remnants in the native tissue and 3D‐dCBS were determined with DNA content analysis and agarose gel electrophoresis. Afterward, 3D‐dCBS were biochemically characterized in depth via glycosaminoglycan (GAG) content, hydroxyproline (HYP) assay, and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). Cellular survival of human adipose‐derived mesenchymal stem cells (hAMSCs) on the 3D‐dCBS for 3rd, 7th, and 10th days was assessed via MTT assay. Scaffold and cell/scaffold constructs were also evaluated with scanning electron microscopy and histochemical studies. DNA contents for native and 3D‐dCBS were respectively found to be 520.76 ± 18.11 and 28.80 ± 0.20 ng/mg dry weight (n = 3), indicating a successful decellularization process. GAG content, HYP assay, and SDS‐PAGE results proved that the extracellular matrix was substantially preserved during the decellularization process. In conclusion, it is believed that the novel decellularization method may allow fabricating 3D bioscaffolds with desired geometry from soft nervous system tissues.

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Development of a novel aptasensor using jellyfish collagen as matrix and thrombin detection in blood samples obtained from patients with various neurodisease

Cited by 42 publications

References 54 publications

Enhancement of aptamer immobilization using egg shell-derived nano-sized spherical hydroxyapatite for thrombin detection in neuroclinic

Enhancement of aptamer immobilization using egg shell-derived nano-sized spherical hydroxyapatite for thrombin detection in neuroclinic

In vivo comparison of jellyfish and bovine collagen sponges as prototype medical devices

A novel method for constructing an acellular 3D biomatrix from bovine spinal cord for neural tissue engineering applications

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