Development of a novel bioactive glass suitable for osteosarcoma‐related bone grafts

Abstract

In this study, zinc borate-based glasses with increasing gallium content (0, 2.5, 5, 10, and 15 wt % Ga) were synthesized and their effect on the viability and proliferation of preosteoblasts and osteosarcoma cancer cells were investigated. Methyl thiazolyl tetrazolium (MTT) cell viability assays using glass degradation extracts revealed that the extracts from glasses with lower Ga contents could enhance the viability of preosteoblasts, while extracts from the glass composition with 15 wt % Ga caused statistically significant reduction of their viability. MTT cell viability assays using the extracts and osteosarcoma cells showed that only extracts from the glass composition with 5 wt % Ga (G3) did not cause a statistically significant increase in the viability of cancer cells for all degradation periods (1 day, 7 days, and 28 days). G3 was selected as the most suitable composition for the osteosarcoma-related graft operations as it could improve the viability of preosteoblasts without increasing the viability of cancer cells. The viability of preosteoblasts and osteosarcoma cells in contact with the glass powders were also investigated using MTT assays. The results showed that the G3 powders could enhance the viability of preosteoblasts while decreasing the viability of osteosarcoma cells. Finally, live/dead assays revealed that suppression of proliferation appeared to be the mechanism causing the observed reductions in the viability of osteosarcoma cells exposed to G3 powders. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1186-1193, 2018.

Bioactive Glasses and Glass-Ceramics for Healthcare Applications in Bone Regeneration and Tissue Engineering

“…This is important to prevent thermal stresses between the glass coatings and this alloy when used in medical implants, which is common.Gallium oxide has been explored as an additive to zinc borate bioactive glasses to confer them with antibacterial properties [199]. The same authors also investigated the suitability of gallium-releasing zinc borate bioactive glasses for osteosarcoma-related bone graft operations using MTT and live/dead assay [200]. They concluded that glass powders containing 5 wt.% of Ga 2 O 3 could enhance the viability of preosteoblasts while reducing that of osteosarcoma cells.…”

In vitro evaluation of novel titania‐containing borate bioactive glass scaffolds

“…Cell viability assays using 3‐(4,5‐dimethylthiazolyl‐2)‐2,5‐diphenyltetrazolium bromide (MTT) assay kits were performed to determine in vitro cytotoxicity of the borate‐based glass scaffolds (Rahimnejad Yazdi, Torkan, Waldman, & Towler, 2018). Pre‐osteoblastic MC3T3‐E1 (ATCC CRL‐2593) cells and glass scaffold degradation extracts were used.…”

“…Fluorescence‐based live‐dead assay (Molecular Probes Inc. OR) with calcein AM for labeling live cells and EthD‐1 for labeling dead cells were used to evaluate the viability of the pre‐osteoblastic MC3T3‐E1 (ATCC CRL‐2593) cells after exposure to glass scaffold degradation extracts (Rahimnejad Yazdi et al, 2018). Similar to MTT assay, pre‐osteoblastic cells were cultured in minimum essential medium (MEM) α supplemented with 10% fetal bovine serum and 1% antibiotic–antimycotic and maintained in a cell culture incubator for 24 hr.…”

Gallium containing bioactive materials: A review of anticancer, antibacterial, and osteogenic properties

“…Decreased osteosarcomas viability using containing 5% wt. Ga 2 O 3 glass extract for 7 days [ 91 ] B 2 O 3 –CaO–Na 2 O–K 2 O–MgO–P 2 O 5 –Ce 2 O 3 (1, 3, 5%)/Ga 2 O 3 (1, 5%) Characterization of scaffold and powder by SEM-EDX, XRD, FTIR, DTA-TG. In vitro bioactivity and degradation studies in SBF.…”

“…A borate glass system (B 2 O 3 –Na 2 O–CaO–P 2 O 5 –ZnO-Ga 2 O 3 ) incorporating up to 16 wt % of gallium was investigated by Yazdi et al [ 91 ]. Degradation rate and weight loss of the glass in deionized water were reduced with gallium addition: it followed to changes in the glass network, where gallium substituted boron [ 92 ].…”