Development of a Novel Composite Material with Carbon Nanotubes Assisted by Self-Assembled Peptides Designed in Conjunction with β-Sheet Formation

Sustained Release of Mitomycin C from Its Conjugate with Single-Walled Carbon Nanotubes Associated by Pegylated Peptide

“…In previous studies, 11,23) a novel composite material was developed using SWCNTs and peptides such as (KF) 14 , (EFEA) 7 , (KFKA) 7 , (KFCA) 7 , and (KFKA) 6 KFCA, which were designed to form a β-sheet structure suitable for wrapping the SWCNTs. The formation of the SWCNTs-peptide composite was confirmed by its performance in water, AFM, transmission electron microscopy observation, and molecular modeling.…”

“…The SWCNTs-CWKG(KWKG) 6 composite was prepared in 5 mL D 2 O solution containing 2 mg SWCNTs and 10 mg CWKG(KWKG) 6 peptide in a glass tube by sonicating for 1 h with an ultrasonic disruptor UD-201 (TOMY Digital Biology, Tokyo, Japan) in an ice water bath. 11,17) The chemical and structural stability of peptides during sonication was demonstrated in our previous study, where a complex formation of peptides with SWCNTs was indicated by atomic force microscopy (AFM) image of SWCNTs-peptide and β-sheet formation of peptide was assigned by circular dichroism (CD) spectrum.…”

“…9,10) In contrast, a novel composite of single-walled carbon nanotubes (SWCNTs) with amphiphilic designed peptides was developed and their chemical and physicochemical characteristics were evaluated with respect to biomedical applications. 11) SWCNTs are one of the most attractive materials in the biomedical field due to their unique cylindrical structure with broad surface areas and physical, chemical, electrical, and mechanical properties. 12,13) In addition, SWCNTs are prominently taken up into cells in vitro via direct penetration or endocytic pathway.…”

“…In SWCNTs composites wrapped with amphiphilic H-(-Lys-Trp-Lys-Gly-) 7 -OH [(KWKG) 7 )] peptides, the (KWKG) 7 peptide interacts with the surface of SWCNTs via π-π interaction at multiple points between the indole skeleton of the tryptophan residue and SWCNTs. 11) Hence, SWCNTs-(KWKG) 7 shows good dispersion stability in water because of electrostatic repulsion between themselves. Moreover, polyethylene glycol (PEG) modifications of the SWCNTs-(KWKG) 7 composites remarkably improved the dispersion stability even under a biological environment, thereby suggesting the tremendous potential of these composites as drug and gene carriers.…”

Development of Novel Drug and Gene Delivery Carriers Composed of Single-Walled Carbon Nanotubes and Designed Peptides With PEGylation

“…4 In our previous studies, we developed novel composite materials of SWCNTs using artificially designed peptides and evaluated their chemical and physicochemical characteristics with an aim toward biomedical application. [8][9][10] In addition to improving their dispersion stability in water, we showed that various functions could be introduced to the SWCNTs-peptide complexes via methods such as the introduction of special amino acids, chemical modification, and additional complex formation based on electrostatic interaction. [8][9][10] These results suggest the potential of the SWCNTs-peptide complex as a molecular platform on which a desirable structure can be constructed and a desirable function introduced for biomedical and industrial application.…”