Development of a novel enzyme-linked immunosorbent assay (ELISA) for measurement of serum CTRP1

Chalupová, Lenka; Žákovská, Alena; Adamcová, K

doi:10.1016/j.clinbiochem.2012.09.006

Cited by 44 publications

(27 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Circulating CTRP1 levels have been reported to significantly increase in subjects with hypertension [14] and metabolic syndrome [15]. In the present study, we explored the relationship between CTRP1 and T2DM.…”

Section: Discussionmentioning

confidence: 95%

“…Levels of adiponectin and CTRP1 were measured with the commercial ELISA kits manufactured by Antibody and Immunoassay Services (the University of Hong Kong, Hong Kong) [21] and Biovendor (Czech Republic) [15], respectively. The CTRP1 assay was highly specific to human CTRP1 without cross-reacting with other family members, and the intra- and inter assay variations were 2.7% and 8.5%, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Recent human studies have indicated that the circulating levels of CTRP1 were significantly increased in subjects with hypertension [14] and metabolic syndrome [15]. However, no data has been available concerning the relationship between CTRP1 and T2DM.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Circulating Complement-C1q TNF-Related Protein 1 Levels Are Increased in Patients with Type 2 Diabetes and Are Associated with Insulin Sensitivity in Chinese Subjects

Pan

Lü

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

BackgroundComplement-C1q TNF-related protein 1 (CTRP1), a member of the CTRP superfamily, possesses anti-inflammatory and anti-diabetic effects in mice. However, the clinical relevance of CTRP1 has been seldom explored. The current study aimed to investigate the association of circulating CTRP1 and type 2 diabetes mellitus (T2DM) in a Chinese population.Design and MethodsSerum CTRP1 and adiponectin levels of 96 T2DM patients and 85 healthy subjects were determined by ELISA, and their associations with adiposity, glucose and lipid profiles were studied. In a subgroup of this study, the 75-g oral glucose tolerance test (OGTT) was performed in 20 healthy and 20 T2DM subjects to evaluate the relationship among serum levels of CTRP1 and adiponectin, insulin secretion and insulin sensitivity.ResultsSerum CTRP1 levels were significantly increased in patients with T2DM, compared with healthy controls (p<0.001). Similar to adiponectin, serum levels of CTRP1 were significantly correlated to several parameters involved in glucose metabolism and insulin resistance, and independently associated with fasting glucose levels (p<0.05) after BMI and gender adjustments. Furthermore, CTRP1 levels were positively correlated to insulin secretion, while negatively to insulin sensitivity, as measured by OGTT.ConclusionCTRP1 is a novel adipokine associated with T2DM in humans. The paradoxical increase of serum CTRP1 levels in T2DM subjects may be due to a compensatory response to the adverse glucose and lipid metabolism, which warrants further investigation.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Circulating Complement-C1q TNF-Related Protein 1 Levels Are Increased in Patients with Type 2 Diabetes and Are Associated with Insulin Sensitivity in Chinese Subjects

Pan

Lü

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Overexpression of CTRP1 also improves insulin sensitivity and glucose tolerance under conditions of obesity [18]. A recent clinical study demonstrated that high levels of circulating CTRP1 are associated with metabolic syndrome [19]. Thus, these observations suggest that CTRP1 is associated with obesity-related metabolic disorders.…”

Section: Introductionmentioning

confidence: 86%

Association of Circulating C1q/TNF-Related Protein 1 Levels with Coronary Artery Disease in Men

et al. 2014

View full text Add to dashboard Cite

ObjectiveObesity is a major risk factor for cardiovascular disease. Recent evidence demonstrates that dysregulation of fat-derived hormones, also known as adipokines, is linked with the pathogenesis of obesity-related disorders including coronary artery disease (CAD). Here, we investigated whether circulating level of an adipokine C1q/TNF-related protein (CTRP) 1 is associated with the prevalence of CAD.Methods and ResultsConsecutive 76 male CAD patients were enrolled from inpatients that underwent coronary angiography. Sixty four healthy male subjects served as controls. Plasma CTRP1 concentration was determined by enzyme-linked immunosorbent assay. CTRP1 levels were correlated positively with systolic blood pressure (BP) and triglyceride levels, and negatively with HDL cholesterol levels in all subjects. Plasma levels of CTRP1 were significantly higher in CAD patients than in control subjects (CAD: 443.3±18.6 ng/ml, control: 307.8±21.5 ng/ml, p<0.001). Multiple logistic regression analysis with body mass index, systolic BP, glucose, total cholesterol, HDL cholesterol, triglyceride, adiponectin and CTRP1 revealed that CTRP1 levels, together with systolic BP and HDL cholesterol, correlated with CAD.ConclusionsOur data indicate the close association of high CTRP1 levels with CAD prevalence, suggesting that CTRP1 represents a novel biomarker for CAD.

show abstract

“…Additionally, patients with metabolic syndrome and type 2 diabetes have increased circulating levels of CTRP-1 compared with healthy individuals. This is positively correlated with BMI, fasting glucose, TNF-α (tumour necrosis factor-alpha) and HBA1c (glycated haemoglobin A1c) (Chalupova et al 2013, Xin et al 2014. Circulating levels of CTRP-1 are also increased in non-obese hypertensive patients; hence, CTRP-1 has been suggested as a critical protein associated with the pathophysiology of obesity-related hypertension (Jeon et al 2008).…”

Section: Ctrp-1 Complement-c1qmentioning

confidence: 97%

Adipocytes, aldosterone and obesity-related hypertension

Cat¹,

Friederich‐Persson²,

White³

et al. 2016

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Aldosterone and obesity a nguyen dinh cat and others 57:1 F7-F21 AbstractUnderstanding the mechanisms linking obesity with hypertension is important in the current obesity epidemic as it may improve therapeutic interventions. Plasma aldosterone levels are positively correlated with body mass index and weight loss in obese patients is reported to be accompanied by decreased aldosterone levels. This suggests a relationship between adipose tissue and the production/secretion of aldosterone. Aldosterone is synthesized principally by the adrenal glands, but its production may be regulated by many factors, including factors secreted by adipocytes. In addition, studies have reported local synthesis of aldosterone in extra-adrenal tissues, including adipose tissue. Experimental studies have highlighted a role for adipocyte-secreted aldosterone in the pathogenesis of obesity-related cardiovascular complications via the mineralocorticoid receptor. This review focuses on how aldosterone secretion may be influenced by adipose tissue and the importance of these mechanisms in the context of obesity-related hypertension.

show abstract

Development of a novel enzyme-linked immunosorbent assay (ELISA) for measurement of serum CTRP1

Cited by 44 publications

References 12 publications

Circulating Complement-C1q TNF-Related Protein 1 Levels Are Increased in Patients with Type 2 Diabetes and Are Associated with Insulin Sensitivity in Chinese Subjects

Circulating Complement-C1q TNF-Related Protein 1 Levels Are Increased in Patients with Type 2 Diabetes and Are Associated with Insulin Sensitivity in Chinese Subjects

Association of Circulating C1q/TNF-Related Protein 1 Levels with Coronary Artery Disease in Men

Adipocytes, aldosterone and obesity-related hypertension

Contact Info

Product

Resources

About