Development of a Novel High‐Performance Thin Layer Chromatography–Based Method for the Simultaneous Quantification of Clinically Relevant Lipids from Cells and Tissue Extracts

Abstract

Lipids such as cholesterol, triacylglycerols, and fatty acids play important roles in the regulation of cellular metabolism and cellular signaling pathways and, as a consequence, in the development of various diseases. It is therefore important to understand how their metabolism is regulated to better define the components involved in the development of various human diseases. In the present work, we describe the development and validation of a high‐performance thin layer chromatography (HPTLC) method allowing the separation and quantification of free cholesterol, cholesteryl esters, nonesterified fatty acids, and triacylglycerols. This method will be of interest as the quantification of these lipids in one single assay is difficult to perform.

Is cholesterol a risk factor for breast cancer incidence and outcome?

“…J o u r n a l P r e -p r o o f 26 Cancer cells also often contain significant amount of cholesteryl ester [113], which may play a role in cancer. In that case, it appears that an accumulation of cholesteryl esters within the breast tumor contributes to its aggressive nature, in particular by stimulating cellular proliferation.…”

Adipocytes Promote Breast Cancer Cell Survival and Migration through Autophagy Activation

“…HPTLC was performed as previously described [29,30]. Briefly, lipid extracts were spotted on HPTLC precoated silica gel glass plates 60F254 (20 × 10 cm; 1.05642.0001; Merck Millipore, Molsheim, France) using a Camag autosampler ATS4 sample applicator (Camag, Muttenz, Switzerland) and compared with standard calibration curves.…”