Development of a Novel Method for the Clinical Visualization and Rapid Identification of Multidrug-Resistant Candida auris

Zhang, X R; Ma, Tian; Wang, Y C; Hu, Shan; Yang, Ying

doi:10.1128/spectrum.04912-22

Cited by 5 publications

(4 citation statements)

References 49 publications

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“…Although the time to results is short and the cost of analysis is affordable (Dhiman et al, 2011 ), this assay still requires an expensive analyzer and trained technicians thus limiting broad availability of the method. Molecular amplification-based methods, such as Polymerase Chain Reaction (PCR) or Loop-Mediated Amplification Method (LAMP), are also powerful tools and accurate for Candida auris identification (Yamamoto et al, 2018 ; Alvarado et al, 2021 ; Mulet Bayona et al, 2021 ; Zhang et al, 2023 ). However, commercially available kits are mainly used in positive blood cultures or other direct samples considering their high cost (Lockhart et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

A lateral flow immunoassay for the rapid identification of Candida auris from isolates or directly from surveillance enrichment broths

Chalin,

Arvor,

Hervault

et al. 2024

Front. Microbiol.

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IntroductionCandida auris is a recently discovered yeast with a multi-drug resistant profile associated with high mortality rates. The rapid identification of Candida auris in hospital settings is crucial to allow appropriate therapeutic and rapid implementation of infection management measures. The aim of this study was to develop a lateral flow immunoassay (LFIA) for the rapid identification of Candida auris.MethodsHighly specific monoclonal antibodies were obtained by immunizing mice with membrane proteins from Candida auris which were then used to develop a LFIA whose performance was assessed by testing 12 strains of Candida auris and 37 strains of other Candida species. Isolates were grown on either Sabouraud dextrose, CHROMagarTM Candida Plus or HardyCHROMTMCandida + auris agar plates. The strains were also cultured on salt sabouraud-dextrose with chloramphenicol or a commercially available Salt-Sabouraud Dulcitol Broth with chloramphenicol and gentamicin, and processed using a simple centrifugation protocol to recover a pellet. Finally, the colonies or yeast extract were transferred to the LFIA to determine the specificity and sensitivity of the assay.ResultsThe LFIA reached 100% specificity and sensitivity from solid agar plates. For both enrichment broths, some Candida non-auris species were able to grow, but the LFIA remained 100% specific. The use of a dextrose-based sabouraud broth resulted in earlier identification with the LFIA, with most of the Candida auris strains detected at 24 h.ConclusionThe developed LFIA prototype represents a powerful tool to fight the emerging threat of Candida auris. Clinical validation represents the next step.

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Section: Discussionmentioning

confidence: 99%

A lateral flow immunoassay for the rapid identification of Candida auris from isolates or directly from surveillance enrichment broths

Chalin,

Arvor,

Hervault

et al. 2024

Front. Microbiol.

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“…A recent study by Jacques F. Meis et al identified C. auris at the level of different clades through high-resolution mass spectrometry ( Jamalian et al, 2023 ) and this provides us with new tools for faster and more accurate identification for a rapid response to nosocomial C. auris infection and to trace the source of the infection. In addition, YANG et al applied recombinase-aided amplification combined with lateral flow strips (RAA-LFS) to detect C. auris and successfully developed a rapid and effective method for this purpose ( Zhang et al, 2023 ). In conclusion, C. auris identification methods that are faster, cheaper, and have higher resolutions remain the focus of future research.…”

Section: Discussionmentioning

confidence: 99%

Global characteristics and trends in research on Candida auris

Wang,

Cheng,

Wang

et al. 2023

Front. Microbiol.

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IntroductionCandida auris, a fungal pathogen first reported in 2009, has shown strong resistance to azole antifungal drugs and has caused severe nosocomial outbreaks. It can also form biofilms, which can colonize patients’ skin and transmit to others. Despite numerous reports of C. auris isolation in various countries, many studies have reported contradictory results.MethodA bibliometric analysis was conducted using VOSviewer to summarize research trends and provide guidance for future research on controlling C. auris infection. The analysis revealed that the United States and the US CDC were the most influential countries and research institutions, respectively. For the researchers, Jacques F. Meis published the highest amount of related articles, and Anastasia P. Litvintseva’s articles with the highest average citation rate. The most cited publications focused on clade classification, accurate identification technologies, nosocomial outbreaks, drug resistance, and biofilm formation. Keyword co-occurrence analysis revealed that the top five highest frequencies were for ‘drug resistance,’ ‘antifungal susceptibility test,’ ‘infection,’ ‘Candida auris,’ and ‘identification.’ The high-frequency keywords clustered into four groups: rapid and precise identification, drug resistance research, pathogenicity, and nosocomial transmission epidemiology studies. These clusters represent different study fields and current research hotspots of C. auris.ConclusionThe bibliometric analysis identified the most influential country, research institution, and researcher, indicating current research trends and hotspots for controlling C. auris.

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“…From these 62 articles, only 25 represented original research papers [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ].…”

Section: Molecular Assays Used For the Active Surveillance Of ...mentioning

confidence: 99%

Candida auris Updates: Outbreak Evaluation through Molecular Assays and Antifungal Stewardship—A Narrative Review

Ionescu,

Luchian,

Damian

et al. 2024

CIMB

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Candida auris was reported by the WHO as second to Cryptococcus neoformans, in the list of nineteen fungal priority pathogens, along with two species with a new nomenclature, Nakaseomyces glabrata (Candida glabrata) and Pichia kudriavzevii (Candida krusei). This novel classification was based on antifungal resistance, the number of deaths, evidence-based treatment, access to diagnostics, annual incidence, and complications and sequelae. We assessed which molecular assays have been used to diagnose Candida auris outbreaks in the last five years. Using “Candida auris; outbreak; molecular detection” as keywords, our search in PubMed revealed 32 results, from which we selected 23 original papers published in 2019–2024. The analyzed studies revealed that the detection methods were very different: from the VITEK® 2 System to MALDI TOF (Matrix-Assisted Laser Desorption Ionization–Time of Flight), NGS (Next-Generation Sequencing), WGS (Whole Genome Sequencing), and commercially available real-time PCR (Polymerase Chain Reaction) assays. Moreover, we identified studies that detected antifungal resistance genes (e.g., FKS for echinocandins and ERG11 for azoles). The analyzed outbreaks were from all continents, which confirms the capability of this yeast to spread between humans and to contaminate the environment. It is important that real-time PCR assays were developed for accurate and affordable detection by all laboratories, including the detection of antifungal resistance genes. This will allow the fast and efficient implementation of stewardship programs in hospitals.

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Development of a Novel Method for the Clinical Visualization and Rapid Identification of Multidrug-Resistant Candida auris

Abstract: Candida auris is a highly lethal, multidrug-resistant, invasive fungus. However, conventional methods of C. auris identification are time-consuming and laborious and have low sensitivity and high error rates.

Cited by 5 publications

References 49 publications

A lateral flow immunoassay for the rapid identification of Candida auris from isolates or directly from surveillance enrichment broths

A lateral flow immunoassay for the rapid identification of Candida auris from isolates or directly from surveillance enrichment broths

Global characteristics and trends in research on Candida auris

Candida auris Updates: Outbreak Evaluation through Molecular Assays and Antifungal Stewardship—A Narrative Review

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