Development of a paper-based analytical device for colorimetric detection of uric acid using gold nanoparticles–graphene oxide (AuNPs–GO) conjugates

Kumar, Sanjay; Bhushan, Pulak; Bhattacharya, Shantanu

doi:10.1039/c6ay01926a

Cited by 53 publications

(36 citation statements)

References 30 publications

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“…First, graphene oxide (GO) was synthesized using modified Hummer's method. 25 Next, reduced GO was prepared by first, suspending 50 mg of as-prepared GO in 200 mL of Milli-Q ultrapure water. After rigorous stirring and ultrasonication for 30 min, 50 lL of hydrazine monohydrate solution was gradually added.…”

Section: Introductionmentioning

confidence: 99%

Tapered lateral flow immunoassay based point-of-care diagnostic device for ultrasensitive colorimetric detection of dengue NS1

et al. 2018

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Dengue virus, a family member, has emerged as a major worldwide health concern, making its early diagnosis imperative. Lateral flow immunoassays have been widely employed for point-of-care diagnosis of dengue because of their rapid naked eye readouts, ease of use, and cost-effectiveness. However, they entail a drawback of low sensitivity, limiting their usage in clinical applications. Herein, we report a novel lateral flow immunoassay for detection of dengue leveraging on the benefits of gold decorated graphene oxide sheets as detection labels and a tapered nitrocellulose membrane. The developed assay allows for rapid (10 min) and sensitive detection of dengue NS1 with a detection limit of 4.9 ng mL, ∼11-fold improvement over the previously reported values. Additionally, the clinical application of the developed assay has been demonstrated by testing it for dengue virus spiked in human serum. The reported lateral flow immunoassay shows significant promise for early and rapid detection of several target diseases.

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Section: Introductionmentioning

confidence: 99%

Tapered lateral flow immunoassay based point-of-care diagnostic device for ultrasensitive colorimetric detection of dengue NS1

et al. 2018

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“…Three distinguished branches with hydrophobic barriers made of SU-8 photoresist were patterned on chromatography paper using photolithography process and used for glucose and albumin detection. Since the development of 2D-l PAD, extensive research has been carried out in device design, performance improvement and their utilization in various point-of-care applications in the past decades (Cate et al 2014;Kumar et al 2016aKumar et al , b, 2017aKumar et al , b, 2018Morbioli et al 2017;Li et al 2017;You et al 2017;Yeh et al 2017;Whitesides 2018).…”

Section: Paper Microfluidics: Historical Perspectivementioning

confidence: 99%

A Historical Perspective on Paper Microfluidic Based Point-of-Care Diagnostics

Kumar

Bhushan

Ágarwal

et al. 2019

Advanced Functional Materials and Sensors

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Paper-based microfluidic systems have emerged as one of the most favorable technologies used in many potential applications such as point-of-care diagnostics, flexible electronics, energy storage, etc. From the past several decades, paper-based technology has readily accepted in the academic research lab and industries as well. The paper-based devices have changed the life of humankind. The distinguishing characteristics of paper substrate like low cost, biodegradability, biocompatibility, and ease of fabrication helped their adaptability in biosensing applications. This chapter gives a concise overview of the historical perspective of paper-based devices, classification of paper types, and their recent applications.

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“…Viele klassische künstliche Peroxidasen, z. B. Metalloxide (Fe 3 O 4 , FeO x H, Fe 2 O 3 , Co 3 O 4 , ZnO, CuO, Cu 2 O, CeO 2 , CoFe 2 O 4 , WO 3 ), Metallhydroxide, Metallchalkogenide (CoSe 2 , CuS, MoS 2 ), Metallnanopartikel (Pt, Pd, Au, Ag, Fe), Legierungen (PtPd, PdNi, AuPd, AuAg, CuAg, FeCo), Metallionen (Cu 2+[152] ), Komplexe (Hämin, chirale metallosupramolekulare Komplexe, Fe‐Phenanthrolin, Preußischblau), wurden verwendet, um Hybride mit CNMs herzustellen. Wegen ihrer großen spezifischen Oberflächen, ihren ausgezeichneten physikalischen Eigenschaften und ihrer vielseitigen Oberflächenchemie spielten CNMs sehr wichtige Rollen in der Regulierung der enzymatischen Aktivität von...…”

Section: Cnm‐basierte Künstliche Peroxidasenunclassified

“…Eine andere Klasse von nachweisbaren Analyten ist in der Lage, die Oxidationsprodukte aus den kolorimetrischen Reaktionen der CNM‐basierten künstlichen Peroxidasen zu reduzieren; hierzu gehören Ascorbinsäure (AA), Glutathion (GSH), Cystein (Cys), Dopamin (DA) und Harnsäure (UA) . Typischerweise kann die Farbabschwächung von oxTMB bei Einwirkung dieser reduzierenden Moleküle mit dem blanken Auge verfolgt werden (Table S3 in den Hintergrundinformationen).…”

Section: Anwendungen Von Cnm‐basierten Künstlichen Peroxidasenunclassified

Kohlenstoff‐Nanozyme: Enzymatische Eigenschaften, Katalysemechanismen und Anwendungen

Sun

Ren

2018

Angewandte Chemie

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Nanozyme haben spezifische Vorteile gegenüber natürlichen Enzymen, wie ihre einfache Produktion in großen Mengen, lange Lagerzeiten, niedrige Kosten und hohe Stabilität unter harschen Umgebungen. Kohlenstoffnanomaterialien (CNMs), einschließlich Fullerenen, Kohlenstoffnanoröhren, Graphen, Kohlenstoffquantenpunkten und Graphenquantenpunkten, sind zu einer herausragenden Substanzfamilie in den Materialwissenschaften geworden. Über die katalytischen Aktivitäten von CNMs und ihren Hybridverbindungen als eine neue Klasse von Nanozymen wurde ausführlich berichtet. In diesem Kurzaufsatz werden die jüngsten Fortschritte bei CNM‐basierten künstlichen Enzymen zusammengefasst, mit einem Schwerpunkt auf ihrer Peroxidase‐artigen Aktivität. Die enzymatischen Eigenschaften, Katalysemechanismen und neuartige Anwendungen von CNM‐Nanozymen in der Sensorik, der Therapie und der Umwelttechnologie werden diskutiert. Darüber hinaus thematisieren wir die verbleibenden Herausforderungen und ungelöste Probleme. Einhergehend mit dem raschen Voranschreiten der Bionanotechnologie haben die einzigartigen enzymatischen Eigenschaften der CNM‐Nanozyme viel Aufmerksamkeit erfahren, und sie werden auch in Zukunft ein aktives Forschungsfeld darstellen.

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Development of a paper-based analytical device for colorimetric detection of uric acid using gold nanoparticles–graphene oxide (AuNPs–GO) conjugates

Abstract: A novel paper-based device for colorimetric detection of uric acid was developed based on the peroxidase-like activity of gold nanoparticles–graphene conjugates (AuNPs–GO).

Cited by 53 publications

References 30 publications

Tapered lateral flow immunoassay based point-of-care diagnostic device for ultrasensitive colorimetric detection of dengue NS1

Tapered lateral flow immunoassay based point-of-care diagnostic device for ultrasensitive colorimetric detection of dengue NS1

A Historical Perspective on Paper Microfluidic Based Point-of-Care Diagnostics

Kohlenstoff‐Nanozyme: Enzymatische Eigenschaften, Katalysemechanismen und Anwendungen

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