Development of a polypyrrole-based human serum albumin sensor

John, Richard; Spencer, Melinda J.; Wallace, Gordon G.; Smyth, Malcolm R.

doi:10.1016/s0003-2670(00)83010-x

Cited by 82 publications

(55 citation statements)

References 12 publications

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“…A human serum albumin (HSA) sensor was constructed that utilized a polypyrrole film that was formed galvanostatically on the surface of a platinum electrode using pyrrole in the presence of the antibody to HSA, antihuman serum albumin [47]. The work was an example of direct incorporation of an antibody into the polymer during synthesis which ,simplifies immunosensor technology.…”

Section: Electropolymerizationmentioning

confidence: 99%

Use of polymer films in amperometric biosensors

1995

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Different types of polymeric films implemented in various capacities in amperometric biosensor design are reviewed. Conducting and nonconducting polymer films and composite films are considered. Methods of film fabrication such as solvent casting, electropolymerization, and adsorption are presented. Film function, including prevention of interference and fouling problems, immobilization of the enzyme or active biocomponent, extension of linear range, or to address biocompatibility problems are described. The use of polymeric films as a medium to incorporate mediators, while an important function, is too vast for review here. The applications of polymer film-modified biosensors for selected analytical determinations are also presented. This review focuses upon the use of polymer films in amperometric biosensors and emphasizes innovative biosensor designs and unique applications, and omits traditional free-standing films unless they are coupled with new types of polymer films or used innovatively.

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Section: Electropolymerizationmentioning

confidence: 99%

Use of polymer films in amperometric biosensors

1995

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“…Electropolymerized ®lms such as polypyrrole (PPy) have been used for the immobilization of biological reagents (e.g., enzymes [12] and antibodies [13]) for biosensor development. There have also been studies of reagent immobilization in PPy ®lms for trace metal complexationaaccumulation with voltammetric [14±17], potentiometric [18] or impedimetric [19] detection of the complexed metal.…”

Section: Introductionmentioning

confidence: 99%

Polypyrrole Films Doped with an Electroactive Sulfonated Chelating Reagent: Electrochemical Characterization and the Detection of Metal Ions

Arrigan¹,

Lowens

1999

Electroanalysis

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Polypyrrole ®lms doped with the electroactive chelating reagent 3-(2-pyridyl)-5,6-diphenyl-4,4H -disulfonate-1,2,4-triazine (PDTDS 2À ) were prepared and characterized. The electroactive azo bond in PDTDS 2À was detected in the polymer ®lm by cyclic voltammetry. The ratio of pyrrole to PDTDS 2À in the ®lms (as evaluated from azo reduction and polymer growth charges) was 11.7 : 1 (s 2X03, n 38), quite close to the expected ratio of 8 : 1 for a doubly charged anion-doped ®lm. The azo bond electrochemistry in the polypyrrole exhibited Nernstian pH behavior (DE f aDpH 7 54.7 AE 1.3 mV dec À1 ). Copper detection by medium-exchange cyclic voltammetry at these ®lms was examined and it was found that both reduction and stripping, oxidation responses were obtained but the latter was better as it was narrower and larger in magnitude. Thinner ®lms gave better copper responses and detection of Cu 2 down to 1610 À6 mol dm À3 was possible at ®lms prepared with a growth charge of about 40 mC. Selectivity was evaluated in mixture experiments and a range of commonly occurring ions did not interfere in the Cu 2 electrochemical detection. The most severe interferents were mercury(II) and EDTA; new voltammetric peaks for Ag(I) and Hg(II) were observed.

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“…Nevertheless, few examples have been devoted to the immobilization of coenzymes, antibodies, cells and biological tissue, illustrating the wide potentialities of this method for biomolecule retention [6][7][8].…”

Section: Introductionmentioning

confidence: 99%