Development of a potentiometric immunosensor for herbicide simazine and its application for food testing

Utilização de técnicas eletroanalíticas na determinação de pesticidas em alimentos

“…Após etapas de separação e "clean up", utilizando coluna C 18 , foi obtido limite de detecção de 1,0x10 -8 mol L -1 . Yulaev e colaboradores 53 utilizaram um biossensor potenciométrico com enzima peroxidase para detecção de simazina em alimentos. Este biossensor foi baseado na detecção potenciométrica da água oxigenada gerada pela reação da enzima peroxidase com o substrato, que é inibida após a reação competitiva com o pesticida.…”

Recent Advances in Electrochemical Enzyme Immunoassays

“…Even though amperometric detection is the most common, examples of voltammetric [90, 105, 107, 133 -136, 140, 147, 148, 150], potentiometric [94,153,156,181,182] or impedimetric [151,152] enzyme immunosensors have also been found in the literature in last years.…”

“…First strategy [94,103] usually requires the use of solutions of high/low pH, or high ionic strength, which may not only cleave the bonds between the immunocomplexes, but can also denature the immobilized immunoreagent. A very interesting alternative to the use of these solutions have been proposed by Yoon et al [96] based on a bound-protein displacement reaction.…”

Fabrication of Herbicide Biosensors Based on the Inhibition of Enzyme Activity That Catalyzes the Scavenging of Hydrogen Peroxide in a Thylakoid Membrane

“…Several kinds of electrochemical sensors for the determination of herbicide have been reported. [6][7][8][9][10][11][12][13][14][15][16][17] Most of them were based on enzyme-linking immunoassay, [7][8][9][10] in which herbicides were detected by competitive reactions with labeled antibodies. Although the immunoassay permits one to detect herbicide with high sensitivity, the procedure is complicated, and it is difficult to monitor mixed herbicides because of the specificity of antibodies.…”