2021
DOI: 10.1016/j.bej.2020.107900
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Development of a protein-solubilizing expression method based on the synergistic action of intein ΔI-CM and the solubility enhancer elastin-like polypeptide

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Cited by 3 publications
(1 citation statement)
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“…Mini-Intein ∆I-CM, an intronic peptide with self-cleaving activity at the C-terminal only, was developed by Wood et al 23 . Then Sun et al established a novel soluble protein expression system based on the synergistic interaction of elastin-like polypeptide (ELP) and Mini-Intein ∆I-CM, the fusion of which allowed the target protein to be directly separated from ELP in a mild and enzyme-free environment, allowing for more e cient isolation and puri cation of the target protein 24 .…”
Section: Introductionmentioning
confidence: 99%
“…Mini-Intein ∆I-CM, an intronic peptide with self-cleaving activity at the C-terminal only, was developed by Wood et al 23 . Then Sun et al established a novel soluble protein expression system based on the synergistic interaction of elastin-like polypeptide (ELP) and Mini-Intein ∆I-CM, the fusion of which allowed the target protein to be directly separated from ELP in a mild and enzyme-free environment, allowing for more e cient isolation and puri cation of the target protein 24 .…”
Section: Introductionmentioning
confidence: 99%