Development of a quantitative colorimetric LAMP assay for fast and targeted molecular detection of the invasive lionfishPterois milesfrom environmental DNA
Katherine Hartle-Mougiou,
Chrysoula Gubili,
Panagiota Xanthopoulou
et al.
Abstract:The Mediterranean basin has seen an increased influx of invasive species since the Suez Canal expansion in 2015. The invasive lionfish species,Pterois miles, has rapidly established new populations in the Eastern Mediterranean Sea, impacting local fish biodiversity. Here, we have developed a new, fast (< 35 min) molecular approach to detect and quantifyP. milesenvironmental DNA (eDNA) in combination with a portable device for field-based analysis. Using a species-specific real-time colorimetric loop-mediate… Show more
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