Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation

Schwendimann, Livia; Kauf, Peter; Fieseler, Lars; Gantenbein-Demarchi, Corinne; Schwenninger, Susanne Miescher

doi:10.1016/j.mimet.2015.05.022

Cited by 27 publications

(13 citation statements)

References 27 publications

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“…The following references appear in the Supplemental Information: (Furet et al, 2004), (Haarman and Knol, 2005), (Herbel et al, 2013), (Ruggirello et al, 2014), and (Schwendimann, et al, 2015). (Caporaso et al, 2010) Trimmomatic N/A (Bolger et al, 2014) MetaPhlAn2 N/A (Truong et al, 2015) Bowtie2 N/A (Langmead and Salzberg, 2012) EMPANADA N/A (Manor and Borenstein, 2017) RNASeq analysis software GOrilla (Gene Ontology enRIchment anaLysis and visuaLizAtion tool) http://cbl-gorilla.cs.technion.ac.il/ (Eden et al, 2009) CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for reagents may be directed to and will be fulfilled by Eran Elinav (eran.elinav@weizmann.ac.il).…”

Section: Declaration Of Interestsmentioning

confidence: 99%

Personalized Gut Mucosal Colonization Resistance to Empiric Probiotics Is Associated with Unique Host and Microbiome Features

Zmora

Zilberman-Schapira

Suez

et al. 2018

Cell

1,144

821

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Empiric probiotics are commonly consumed by healthy individuals as means of life quality improvement and disease prevention. However, evidence of probiotic gut mucosal colonization efficacy remains sparse and controversial. We metagenomically characterized the murine and human mucosal-associated gastrointestinal microbiome and found it to only partially correlate with stool microbiome. A sequential invasive multi-omics measurement at baseline and during consumption of an 11-strain probiotic combination or placebo demonstrated that probiotics remain viable upon gastrointestinal passage. In colonized, but not germ-free mice, probiotics encountered a marked mucosal colonization resistance. In contrast, humans featured person-, region- and strain-specific mucosal colonization patterns, hallmarked by predictive baseline host and microbiome features, but indistinguishable by probiotics presence in stool. Consequently, probiotics induced a transient, individualized impact on mucosal community structure and gut transcriptome. Collectively, empiric probiotics supplementation may be limited in universally and persistently impacting the gut mucosa, meriting development of new personalized probiotic approaches.

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Section: Declaration Of Interestsmentioning

confidence: 99%

Personalized Gut Mucosal Colonization Resistance to Empiric Probiotics Is Associated with Unique Host and Microbiome Features

Zmora

Zilberman-Schapira

Suez

et al. 2018

Cell

1,144

821

View full text Add to dashboard Cite

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“…The following references appear in the Supplemental Information: Furet et al (2004), Haarman and Knol (2005), Herbel et al (2013), Ruggirello et al (2014), and Schwendimann et al (2015). (Huber et al, 2007) N/A 16S qPCR 112-967F-UC3:ATACGCGARGAACCTTACC (Huber et al, 2007) N/A 16S qPCR 113-967F-AQ:CTAACCGANGAACCTYACC (Huber et al, 2007) N/A 16S qPCR 114-967F-S:CAACGCGMARAACCTTACC (Huber et al, 2007) N/A 16S qPCR 115-1046R-S:CGACRRCCATGCANCACCT (Huber et al, 2007)…”

Section: Declaration Of Interestsmentioning

confidence: 99%

Post-Antibiotic Gut Mucosal Microbiome Reconstitution Is Impaired by Probiotics and Improved by Autologous FMT

Suez

Zmora

Zilberman-Schapira

et al. 2018

Cell

844

590

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Probiotics are widely prescribed for prevention of antibiotics-associated dysbiosis and related adverse effects. However, probiotic impact on post-antibiotic reconstitution of the gut mucosal host-microbiome niche remains elusive. We invasively examined the effects of multi-strain probiotics or autologous fecal microbiome transplantation (aFMT) on post-antibiotic reconstitution of the murine and human mucosal microbiome niche. Contrary to homeostasis, antibiotic perturbation enhanced probiotics colonization in the human mucosa but only mildly improved colonization in mice. Compared to spontaneous post-antibiotic recovery, probiotics induced a markedly delayed and persistently incomplete indigenous stool/mucosal microbiome reconstitution and host transcriptome recovery toward homeostatic configuration, while aFMT induced a rapid and near-complete recovery within days of administration. In vitro, Lactobacillus-secreted soluble factors contributed to probiotics-induced microbiome inhibition. Collectively, potential post-antibiotic probiotic benefits may be offset by a compromised gut mucosal recovery, highlighting a need of developing aFMT or personalized probiotic approaches achieving mucosal protection without compromising microbiome recolonization in the antibiotics-perturbed host.

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“…DNA extractions were performed on 500 mg of feces per sample using the FastDNA SPIN Kit for Soil (MP Biomedicals, Solon, CA, USA). L. fermentum LM1016 abundance was determined using a quantitative real-time PCR (qPCR) assay with L. fermentum-specific primers 23 and normalized to 16S rRNA-specific primers 24 . Taq-Man™ Fast Advanced Master Mix (Applied Biosystems, Foster City, CA, USA) and the StepOnePlus real-time PCR system (Applied Biosystems) were used for qPCR experiments.…”

Section: Colonization Of Lactobacillus Fermentum Lm1016mentioning

confidence: 99%

Lactobacillus fermentum promotes adipose tissue oxidative phosphorylation to protect against diet-induced obesity

et al. 2020

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The gut microbiota has pivotal roles in metabolic homeostasis and modulation of the intestinal environment. Notably, the administration of Lactobacillus spp. ameliorates diet-induced obesity in humans and mice. However, the mechanisms through which Lactobacillus spp. control host metabolic homeostasis remain unclear. Accordingly, in this study, we evaluated the physiological roles of Lactobacillus fermentum in controlling metabolic homeostasis in diet-induced obesity. Our results demonstrated that L. fermentum-potentiated oxidative phosphorylation in adipose tissue, resulting in increased energy expenditure to protect against diet-induced obesity. Indeed, oral administration of L. fermentum LM1016 markedly ameliorated glucose clearance and fatty liver in high-fat diet-fed mice. Moreover, administration of L. fermentum LM1016 markedly decreased inflammation and increased oxidative phosphorylation in gonadal white adipose tissue, as demonstrated by transcriptome analysis. Finally, metabolome analysis showed that metabolites derived from L. fermentum LM1016-attenuated adipocyte differentiation and inflammation in 3T3-L1 preadipocytes. These pronounced metabolic improvements suggested that the application of L. fermentum LM1016 could have clinical applications for the treatment of metabolic syndromes, such as diet-induced obesity.

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Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation

Cited by 27 publications

References 27 publications

Personalized Gut Mucosal Colonization Resistance to Empiric Probiotics Is Associated with Unique Host and Microbiome Features

Personalized Gut Mucosal Colonization Resistance to Empiric Probiotics Is Associated with Unique Host and Microbiome Features

Post-Antibiotic Gut Mucosal Microbiome Reconstitution Is Impaired by Probiotics and Improved by Autologous FMT

Lactobacillus fermentum promotes adipose tissue oxidative phosphorylation to protect against diet-induced obesity

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