Development of a QuEChERS-based extraction method for the determination of destruxins in potato plants by UHPLC–MS/MS

Carpio, Azahara; Arroyo‐Manzanares, Natalia; Ríos-Moreno, A.; Garrido‐Jurado, Inmaculada; Gámiz‐Gracia, Laura; Garcı́a-Campaña, Ana M.; Quesada‐Moraga, Enrique; Arce, Lourdes

doi:10.1016/j.talanta.2015.06.008

Cited by 15 publications

(3 citation statements)

References 28 publications

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“…On the other hand, their presence in food products is a concern as they can pose a threat to human health and disturb the balance of the ecosystem (Taibon et al 2015). Dtxs have been found in strawberries, maize (Taibon et al 2015), and potatoes (Carpio et al 2016).…”

Section: Secondary Metabolites: Role In Infection and Possible Applicmentioning

confidence: 99%

Entomopathogenic fungi: unconventional applications

Litwin

Nowak

Różalska

2020

Rev Environ Sci Biotechnol

178

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Entomopathogenic fungi (EPF) are microorganisms that cause fatal diseases of arthropods. The infection process involves several stages that consist of direct contact of the fungus with the surface of the cuticle of the attacked insect. The factors that determine the effectiveness of the infection process include lytic enzymes, secondary metabolites, and adhesins produced by EPF. Because of their high insecticidal effectiveness, these fungi are commonly used as biopesticides in organic farming. As the environment and farmlands are contaminated with many compounds of anthropogenic origin (e.g., pesticides), the effects of these toxic compounds on EPF and the mechanisms that affect their survival in such a toxic environment have been studied in recent years. This review presents information on the capacity of EPF to remove toxic contaminants, including alkylphenols, organotin compounds, synthetic estrogens, pesticides and hydrocarbons. Moreover, these fungi produce numerous secondary metabolites that can be potentially used in medicine or as antimicrobial agents. Despite their huge potential in biocontrol processes, the use of EPF has been underestimated due to a lack of knowledge on their abilities. In our work, we have presented the available data on the possibilities of the additional and unconventional use of these microorganisms.

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Section: Secondary Metabolites: Role In Infection and Possible Applicmentioning

confidence: 99%

Entomopathogenic fungi: unconventional applications

Litwin

Nowak

Różalska

2020

Rev Environ Sci Biotechnol

178

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“…Sample preparation is an essential step in the LC-MS/MS analysis of complex matrix effect samples, and solid-phase extraction (SPE) and QuEChERS are the most widely used pretreatment methods. − SPE can provide good purification, but the procedure is usually costly, complicated, and time-consuming, which limits the pretreatment speed when working with a large number of samples containing multiple mycotoxins. The traditional QuEChERS method is carried out using salts to extract and DSPE adsorbents, including primary secondary amine (PSA), graphitized carbon black (GCB), and C18, for purification. − However, effective purification cannot be achieved by conventional DSPE adsorbents (PSA, GCB, and C18) in ultratrace simultaneous determination of mycotoxins, especially for difficult matrices like grains, which leads to unsatisfying LODs and restricts the widespread application of the QuEChERS method .…”

Section: Introductionmentioning

confidence: 86%

Using Magnetic Multiwalled Carbon Nanotubes as Modified QuEChERS Adsorbent for Simultaneous Determination of Multiple Mycotoxins in Grains by UPLC-MS/MS

Wang²,

You

et al. 2019

J. Agric. Food Chem.

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The simultaneous detection of multiple mycotoxins is important due to the increased toxic effects of combined mycotoxins in grains. In this research, a combination of modified QuEChERS with ultrahigh-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) was used for simultaneous detection of 20 mycotoxins in grains. A series of different types of magnetic (Fe3O4) nanoparticles modified with multiwalled carbon nanotubes (Fe3O4-MWCNTs) were designed as modified QuEChERS adsorbents for facile and efficient purification and for target interferences removal in the matrices. When there is an external magnetic field, the proposed modified QuEChERS method uses a shorter pretreatment time compared with the traditional QuEChERS method, which makes it possible to conduct high-throughput analyses. To optimize the QuEChERS process, the extraction solvent and the type and amount of the Fe3O4-MWCNTs were investigated. Under optimal conditions, the method was validated and showed satisfactory linearity (r 2 ≥ 0.9965), good recovery (73.5–112.9%), good precision (1.3–12.7%), and excellent sensitivity (ranging from 0.0021 to 5.4457 ng g–1), which indicates that this method can be used for detecting multiple mycotoxins in real samples.

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“…Since its inception, the QuEChERS method has been developed to analyze a wide range of compounds with high recovery, precision, accuracy, speed, and convenient operation. Recently, this method has been widely used in the determination of a variety of chemicals, including veterinary residues , food contaminants , and several active metabolites in produce . In this work, a new QuEChERS‐based method was developed and validated for the multiclass determination of 28 PGRs having various chemical characteristics.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of multiclass plant growth regulators in fruits using the quick, easy, cheap, effective, rugged, and safe method and ultra‐high performance liquid chromatography‐tandem mass spectrometry

Xu¹,

Hou

Mei

et al. 2019

J of Separation Science

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In this study, a modified quick, easy, cheap, effective, rugged, and safe method combined with ultra‐high performance liquid chromatography and tandem mass spectrometry was developed for the multiclass determination of 28 plant growth regulators in various fruits. Different extraction solvents and adsorbents, including primary secondary amine, octadecylsilyl, graphitized carbon black, and zirconia‐based sorbent, were investigated. Internal calibration and isotope internal standards, chlormequat chloride‐d4, mepiquat chloride‐d6, indole‐3‐acetic acid‐d2, and forchlorfenuron‐d5 were used to improve accuracy. For method validation, good linearity, low limits of detection and quantification were obtained. At three spiked concentrations (10, 50, and 100 µg/kg), satisfactory recoveries with relative standard deviations of 2.4–17.5% were obtained for strawberries (75.2–119.8%), grapes (70.5–114.0%), tangerines (71.7–115.4%), apples (72.7–115.4%), and kiwi fruits (71.7–119.2%). Samples analysis revealed that 15.6% of the samples (n = 96) were contaminated with one or two kinds of plant growth regulators, including chlormequat chloride, forchlorfenuron, paclobutrazol, 2,4‐dichlorophenoxyacetic acid, 2‐diethylaminoethyl hexanoate, and mepiquat chloride. Similar results were obtained by ultra‐high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry, indicating the robustness, effectiveness, and suitability of the developed method for routine monitoring of plant growth regulator residues in fruits.

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Development of a QuEChERS-based extraction method for the determination of destruxins in potato plants by UHPLC–MS/MS

Cited by 15 publications

References 28 publications

Entomopathogenic fungi: unconventional applications

Entomopathogenic fungi: unconventional applications

Using Magnetic Multiwalled Carbon Nanotubes as Modified QuEChERS Adsorbent for Simultaneous Determination of Multiple Mycotoxins in Grains by UPLC-MS/MS

Simultaneous determination of multiclass plant growth regulators in fruits using the quick, easy, cheap, effective, rugged, and safe method and ultra‐high performance liquid chromatography‐tandem mass spectrometry

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