Development of a rapid dot-blot assay for ochratoxin A (OTA) detection using peptide conjugated gold nanoparticles for bio-recognition and detection

Rahi, Shraddha; Lanjekar, Vikram; Ghormade, Vandana

doi:10.1016/j.foodcont.2022.108842

Cited by 8 publications

(3 citation statements)

References 50 publications

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“…This technique employs light scattering as well as Brownian motion to estimate NP size distribution of samples. The size analysis of the NPs was carried out through nanotracking analysis (NTA 2.3, UK) ( Rahi et al, 2022 ). The sample was loaded in liquid suspension into a chamber with a laser beam for sample illumination scattering the laser light.…”

Section: Methodsmentioning

confidence: 99%

Synergistic Activity of Rhamnolipid Biosurfactant and Nanoparticles Synthesized Using Fungal Origin Chitosan Against Phytopathogens

Karamchandani

Maurya

Dalvi

et al. 2022

Front. Bioeng. Biotechnol.

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Phytopathogens pose severe implications in the quantity and quality of food production by instigating several diseases. Biocontrol strategies comprising the application of biomaterials have offered endless opportunities for sustainable agriculture. We explored multifarious potentials of rhamnolipid-BS (RH-BS: commercial), fungal chitosan (FCH), and FCH-derived nanoparticles (FCHNPs). The high-quality FCH was extracted from Cunninghamella echinulata NCIM 691 followed by the synthesis of FCHNPs. Both, FCH and FCHNPs were characterized by UV-visible spectroscopy, DLS, zeta potential, FTIR, SEM, and Nanoparticle Tracking Analysis (NTA). The commercial chitosan (CH) and synthesized chitosan nanoparticles (CHNPs) were used along with test compounds (FCH and FCHNPs). SEM analysis revealed the spherical shape of the nanomaterials (CHNPs and FCHNPs). NTA provided high-resolution visual validation of particle size distribution for CHNPs (256.33 ± 18.80 nm) and FCHNPs (144.33 ± 10.20 nm). The antibacterial and antifungal assays conducted for RH-BS, FCH, and FCHNPs were supportive to propose their efficacies against phytopathogens. The lower MIC of RH-BS (256 μg/ml) was observed than that of FCH and FCHNPs (>1,024 μg/ml) against Xanthomonas campestris NCIM 5028, whereas a combination study of RH-BS with FCHNPs showed a reduction in MIC up to 128 and 4 μg/ml, respectively, indicating their synergistic activity. The other combination of RH-BS with FCH resulted in an additive effect reducing MIC up to 128 and 256 μg/ml, respectively. Microdilution plate assay conducted for three test compounds demonstrated inhibition of fungi, FI: Fusarium moniliforme ITCC 191, FII: Fusarium moniliforme ITCC 4432, and FIII: Fusarium graminearum ITCC 5334 (at 0.015% and 0.020% concentration). Furthermore, potency of test compounds performed through the in vitro model (poisoned food technique) displayed dose-dependent (0.005%, 0.010%, 0.015%, and 0.020% w/v) antifungal activity. Moreover, RH-BS and FCHNPs inhibited spore germination (61–90%) of the same fungi. Our efforts toward utilizing the combination of RH-BS with FCHNPs are significant to develop eco-friendly, low cytotoxic formulations in future.

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Section: Methodsmentioning

confidence: 99%

Synergistic Activity of Rhamnolipid Biosurfactant and Nanoparticles Synthesized Using Fungal Origin Chitosan Against Phytopathogens

Karamchandani

Maurya

Dalvi

et al. 2022

Front. Bioeng. Biotechnol.

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“…These drawbacks make it difficult to meet the need for fast and cost-effective OTA detection. Another commonly utilized detection approach is based on the antigen-antibody specific immune response, such as colloidal-gold immunochromatography assay (GICA) 11 and enzyme-linked immunosorbent assay (ELISA) 12,13 to achieve the detection of OTA. These methods have the advantages of robust specificity, extreme sensitivity, and basic equipment.…”

Section: Introductionmentioning

confidence: 99%

Robust and ultrasensitive electrochemical detection of ochratoxin a using a highly reactive DNAzyme wired via primer exchange reaction

Meng,

Zhang,

Guo

et al. 2024

New J. Chem.

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“…3 Especially, the OTC can be easily converted into OTA after intake even at very low concentrations. 4 Considering the hepatorenal toxicity, 5 teratogenic, carcinogenic, 6 genotoxic, 7 and immunosuppressive 8 of OTA, many countries and institutions have established strict statute on the threshold in various foods, 9 but a clear limit of OTB and OTC has not been built up. It is worth noting that the foods are rarely contaminated with a single OT; if only OTA is detected, the total intake of OTs is often underestimated.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Nanofibers Modified with Copper Nanoparticles for Simultaneous Extraction and Detection of Three Ochratoxins in Foods

Hou,

Chu,

et al. 2024

ACS Appl. Nano Mater.

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In this study, several electrospun nanofibers were prepared and characterized, and the electrospun polystyrene/ poly(ether sulfone) nanofiber coated with copper nanoparticles (PS/PES-CuNP nanofibers) was selected and utilized as solidphase extraction adsorbent. And then, the packed-fiber solid-phase extraction coupled with high-performance liquid chromatographyfluorescence detection method was established for the efficient determination of ochratoxins in foods. With the proposed method, several factors including the type and dosage of nanofibers, sample pH, extraction time, type, and volume of elution solvent were optimized. The results suggested that low limit of detection (0.102−0.126 ng/mL), limit of quantification (0.382−0.436 ng/ mL), and recoveries (85.5−111.1%) for ochratoxin A, B, and C with relative standard deviations <7% were achieved. As-synthesized PS/PES-CuNP nanofibers displayed satisfactory potential practical application in the simultaneous pretreatment and determination of mycotoxins in complex matrice samples.

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Development of a rapid dot-blot assay for ochratoxin A (OTA) detection using peptide conjugated gold nanoparticles for bio-recognition and detection

Cited by 8 publications

References 50 publications

Synergistic Activity of Rhamnolipid Biosurfactant and Nanoparticles Synthesized Using Fungal Origin Chitosan Against Phytopathogens

Synergistic Activity of Rhamnolipid Biosurfactant and Nanoparticles Synthesized Using Fungal Origin Chitosan Against Phytopathogens

Robust and ultrasensitive electrochemical detection of ochratoxin a using a highly reactive DNAzyme wired via primer exchange reaction

Electrospun Nanofibers Modified with Copper Nanoparticles for Simultaneous Extraction and Detection of Three Ochratoxins in Foods

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