Development of a rapid resolution HPLC method for the separation and determination of 17 phenolic compounds in crude plant extracts

Abstract

Abstract:© Versita Sp. z o.o. Keywords: Plant phenolics • Rapid Resolution HPLC • Medicinal plantRapid Resolution HPLC/DAD method, on a 1.8 μm, 4.6×50 mm column, was developed to enable a rapid separation of a mixture of 17 compounds, which consisted of hydroxybenzoic acids, hydroxycinnamic acids, flavones, flavonols, flavanone, flavonol-glycoside and antraquinone, in a single run, within 22 minutes. The developed method is precise, accurate and sensitive enough for simultaneous quantitative evaluation of major compounds in crude and hydrolyzed extracts of parsley, buckthorn, mint, caraway and birch. In order to overcome the inability to quantify all the phenolic compounds present in the samples caused by lack of external standards, HPLC approaches for the total phenolic content estimation based on sum of all integrated peak areas were made. These results were compared with the total phenolic content determined by Folin-Cioacalteu method. Although the correlation between the series of data was not significant (p<0.05), the difference between the results of total phenolic content obtained spectrophotometrically and by HPLC was not high in the case of parsley, buckthorn and mint extract. Regarding the obtained results, the HPLC approach could serve as an excellent tool for total phenolic content estimation, without the need for complete identification of the individual compounds.

Prevalence of multidrug resistance in human pathogenic Staphylococcus aureus and their sensitivity to Allamanda cathartica L. leaf extract

“…The crude leaf extract of A. cathartica L. was subjected to initial separation by TLC where Benzene : Ethyl acetate (1:1) solvent systems exhibited most effective separation with three bands. However, it was our primary attempt to separate active single compound from the crude leaf extract but to identify and characterize the bioactive compound more preciously, the crude leaf extract of A. cathartica L. can be further subjected to other chromatographic techniques (Mišan et al, 2011;Sasidharan et al, 2011;Patra et al, 2012).…”

Antihyperlipidemic, antioxidant and weightlowering effects of “Vitalplant”

“…"Vitalplant" mixture contains a wide range of phenolic compounds, free phenolic acids and phenolic acids derivatives. Flavonoids are mostly presented in glycosidic form [8].…”

“…Selected medicinal plants and the mixture were already shown to be a rich source of plant phenolics [8] and to possess antioxidant activity by in vitro tests [7]. A relatively high in vitro antioxidant activity of "Vitalplant" mixture was explained by synergistic effects of its components [7].…”

“…A relatively high in vitro antioxidant activity of "Vitalplant" mixture was explained by synergistic effects of its components [7]. In addition, regardless of the way it is incorporated into cookies, either in the form of alcoholic extracts or as a powdered mixture, "Vitalplant" addition was shown to be efficient in improving antioxidant activity and oxidative stability of the cookies in a dose-dependent manner [7][8][9].…”

Preliminary results of the effect of storage on functional properties of buckwheat

“…The extraction of phenolic compounds from the flour was performed in accordance with the method described in Jambrec et al (2015), while the HPLC/DAD analysis was consistent with the procedure argued by Mišan et al (2011). The procedure is shown in Figure 1.…”