Development of a recovery process for novobiocin

Abstract

SummaryA periodic countercurrent whole beer process was developed for the recovery of novobiocin to eliminate the high cost of mycelium filtration and accompanying antibiotic losses in the filter cake. In such a process screened, but unfiltered, novobiocin fermentation beer is contacted with a special grade of an anionicexchange resin in a series of specially designed, well mixed columns. Each column is fitted with a screen sized to retain resin within the column but allow the cells to pass. Periodically the lead column is isolated from the column train, washed free of beer solids, and eluted. A freshly eluted column is placed in the trail position to allow countercurrent operation. The eluate is then processed to crystalline product.A mathematical model for the sorption of novobiocin was developed based on a suitable continuity equation and mass transfer and equilibrium relationships determined in the laboratory. Digital computations of this model correlated well with laboratory and pilot plant data, and predicted well the performance of the production units. This simulation has been in continued use to predict and reoptimize plant operation as process changes (such as improved beer titers, increased production rate, and the evaluation of superior ion-exchange resins) occurred or were anticipated.

Development of operating conditions for protein purification using expanded bed techniques: The effect of the degree of bed expansion on adsorption performance

“…However, feedstocks containing particulates can be processed directly using fluidized or expanded bed adsorption procedures which are able to operate efficiently in the presence of particulates. 5,[8][9][10][11][12]22,23,27,37,44,48 G enerally, the behavior of expanded beds is different from conventional liquid-solid fluidized beds where both liquid and adsorbent phases are well mixed, which results in poor adsorption performance and low prod~ctivity. '~ In our work, a stable bed of adsorbent beads can be easily established without need to stabilize a fluidized bed using magnetic Previous work has described the operating principles of expanded bed ad~orption.~.…”

Separation of residual fatty acids from aqueous solutions using an agitated solution of protein and membrane filtration

“…There can be more than one mechanism and resistances to mass transfer, but their combined effect can be quantified in terms of an overall mass transfer coefficient and a concentration difference [24,25]. The mass transfer to albumin may be modeled using a linear driving force (LDF) model, which is simple and physically consistent [26].…”

Theory of Linear and Nonlinear Chromatography

“…Predictions from the equilibrium model lead to a shock. The stoichiometric time (or retention time in chromatography) is: (10) where't = L/u is the space time and ~ = m (1-£)/£ is the capacity factor.…”

“…Since percolation processes involve mass transfer between a fluid phase and a support it is possible to identify a solute, a solvent and a diluent as shown in Table 1. Recovery of novobiocin fermentation broth novobiocin ion exchange resin, [10] anionic form…”

“…The objectives of a chromatographic or percolation process can be very different [9]: i) separation of products, e.g., chromatographic analysis, large-scale chromatography; ii) purification of diluents, e.g., removal of pollutants to clean a stream; and iii) recovery of solutes, e.g., recovery of metals from a solution, recovery of antibiotics from fermentation broths [10]. Since percolation processes involve mass transfer between a fluid phase and a support it is possible to identify a solute, a solvent and a diluent as shown in Table 1.…”