Development of a reference measurement procedure for the determination of methylmercury in fish products

Sannac, Sébastien; Fisicaro, Paola; Labarraque, Guillaume; Pannier, Florence; Potin‐Gautier, Martine

doi:10.1007/s00769-009-0509-8

Cited by 10 publications

(4 citation statements)

References 10 publications

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“…Finally, Sannac and co-workers evaluated the uncertainty budgets in the determination of selenomethionine in nutritional supplements 109 and methylmercury in fish products. 110 In both studies it was observed that the factors linked to the measurement, i.e. method repeatability and instrumental variation accounted for more than 70% of the overall uncertainty and 20% from the overall method repeatability.…”

Section: Uncertainty Propagation Studiesmentioning

confidence: 92%

Recent advances in isotope dilution analysis for elemental speciation

Rodríguez-González

Alonso

2010

J. Anal. At. Spectrom.

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Section: Uncertainty Propagation Studiesmentioning

confidence: 92%

Recent advances in isotope dilution analysis for elemental speciation

Rodríguez-González

Alonso

2010

J. Anal. At. Spectrom.

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“…After extraction, Hg species were separated and analyzed using a previously published method (Sannac et al, 2009;Gygax et al, 2019;Sannac et al, 2017) by coupling a high performance liquid chromatograph (HPLC 1260 Series, Agilent Technologies, Santa Clara, United States of America) to the ICP-MS (HPLC-ICP-MS). We used a reversed phase C18 column (Zorbax C-18, 4.6 × 50 mm, Agilent Technologies, Santa Clara, United States of America) and a mobile phase consisting of 0.1% (w/v) L-cysteine (98% v/v) and methanol (2% v/v).…”

Section: Hplc-icp-ms Analysesmentioning

confidence: 99%

Organo-mercury species in a polluted agricultural flood plain: Combining speciation methods and polymerase chain reaction to investigate pathways of contamination

Gfeller

Caplette

Frossard

et al. 2022

Environmental Pollution

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“…The similar methods using a reversed-phase column with L-cysteine eluent have been reported. [26][27][28][29][30][31][32] In this study, Hg separation method based on HPLC-ICP-MS was developed for monitoring of Me-Hg and inorganic mercury (i-Hg, Hg(II)) in biological samples.…”

Section: Introductionmentioning

confidence: 99%

A Method for Methylmercury and Inorganic Mercury in Biological Samples Using High Performance Liquid Chromatography- Inductively Coupled Plasma Mass Spectrometry

et al. 2018

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A new determination method was developed for the measurement of methylmercury (Me-Hg) and inorganic mercury (i-Hg) in biological samples using high-performance liquid chromatography -inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) following alkaline extraction. Mercury species in biological samples were extracted with 10 %(w/w) tetramethylammonium hydroxide (TMAH) solution at 80 °C for 2 h. Methylmercury was completely separated from i-Hg by adamantyl type and octadecylsilyl type columns within 6 and 4 min using isocratic elution, respectively. The detection limits (3σ) of an adamantyl type and an octadecylsilyl columns using the proposed system were 0.08 ng g -1 and 0.13 ng g -1 (as Hg), respectively. Inorganic Hg completely separates from Me-Hg without tailing. The proposed determination methods were applied to several biological certified reference materials (CRMs).The measurement results of Me-Hg obtained by the present method were in good agreement within the expanded uncertainties (k=2) with the certified values. The analytical precision (n=3)of Me-Hg was less than 2 %, and the recoveries of Me-Hg and i-Hg were 101±1 % and 103± 3 %, respectively. In addition, this method enables the determination of Me-Hg and i-Hg for 20 samples in one hour.

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Development of a reference measurement procedure for the determination of methylmercury in fish products

Cited by 10 publications

References 10 publications

Recent advances in isotope dilution analysis for elemental speciation

Recent advances in isotope dilution analysis for elemental speciation

Organo-mercury species in a polluted agricultural flood plain: Combining speciation methods and polymerase chain reaction to investigate pathways of contamination

A Method for Methylmercury and Inorganic Mercury in Biological Samples Using High Performance Liquid Chromatography- Inductively Coupled Plasma Mass Spectrometry

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