Development of a Sensitive Luminometric Immunoassay for Determining Baseline Seasonal Changes in Serum Vitellogenin Levels in Male Flounder (<i>Pleuronectes Yokohamae</i>)

Bessho, Hiroki; Iwakami, Sunao; Hiramatsu, Naoshi; Hara, Akihiko; Hashimoto, Shinichi

doi:10.1080/03067310008034128

Cited by 9 publications

(10 citation statements)

References 17 publications

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“…After 6 months on a non-oestrogenic diet, the VTG concentrations in our stock fish were approximately 100ng/ml, and comparable to many of the lower levels reported in the literature. We cannot say, however, that these levels are now normal, as there are very few baseline data to know how our present levels would compare with those in wild fathead minnows or whether they may still be elevated compared with concentrations determined using a more sensitive assay, e.g., a luminometric immunoassay [37].…”

Section: Discussionmentioning

confidence: 79%

Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

Beresford

Brian

Runnalls

et al. 2011

Enviro Toxic and Chemistry

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Abstract-Vitellogenin (VTG) is a precursor of egg-yolk protein and is therefore present at high concentrations in the plasma of female fish. In male fish, VTG concentrations are usually undetectable or low, but can be induced upon exposure to oestrogenic substances either via the water or the diet. This work was carried out to determine the reason for apparently elevated VTG concentrations in unexposed stock male fathead minnow maintained in our laboratory. The results showed clearly that some of the food given to the fish was oestrogenic and that replacement of this with non-oestrogenic food led to a significant reduction in the basal VTG levels measured in male fish after a 6 month period. This reduction in male VTG concentrations drastically increased the sensitivity of the VTG test in further studies carried out with these fish. Moreover, a review of published concentrations of VTG in unexposed male fathead minnow suggests that this problem may exist in other laboratories. The fathead minnow is a standard ecotoxicological fish test species and so these findings will be of interest to any laboratory carrying out fish tests on endocrine disrupting chemicals.

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Section: Discussionmentioning

confidence: 79%

Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

Beresford

Brian

Runnalls

et al. 2011

Enviro Toxic and Chemistry

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“…This induction property of complete Vg (i.e., high induction levels) sets a high valuation on these subtypes as primary biomarkers for the detection of estrogenic activity in aquatic environments, especially for the initial screening of areas that exhibit high estrogenic activities and that require urgent and prioritized attention. Since VgB appeared to be the major Vg subtype in the sole, it can be concluded that the previous surveys by Hashimoto et al (1998Hashimoto et al ( , 2000 and Bessho et al (2000) were reasonably performed in terms of the selection of the biomarker (i.e., the VgB subtype). In contrast, recent studies indicate the importance of the use of multilateral biomarkers for reliable and accurate evaluation of the potential impacts of estrogenic endocrine-disrupting chemicals (Hong et al, 2009).…”

Section: Discussionmentioning

confidence: 92%

“…In addition, VgB appeared to be the major Vg subtype in the sole serum because the precipitin line of VgB (i.e., the precipitin line formed with anti-mullet LvB in IEP of female serum) was remarkably intense compared to those of other Vg subtypes (VgA and VgC). Hashimoto et al (1998Hashimoto et al ( , 2000 and Bessho et al (2000) developed an immunoassay for sole Vg and used this assay to measure Vg levels in wild populations of marbled sole. The anti-sole Lv used in the present study was the same preparation as that utilized to develop the sole Vg assay in these previous studies.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the Lvs identified here were subjected to immunological characterization with respect to the subtype specificity of the sole Lv antiserum used in our previous surveys of environmental estrogen activities in Japanese coastal areas (Hashimoto et al, 1998Bessho et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…This species lives in waters that are less than 100 m deep and that have a sandy-mud bottom (Sakamoto, 1984). In addition to its commercial importance, the marbled sole has been used as a model species for the evaluation of estrogenic activities in aquatic environments (Hashimoto et al, 1998Bessho et al, 2000). In these analyses, Vg levels in the blood of wild male and female sole were measured, and the levels were evaluated as an estrogen-sensitive biomarker.…”

Section: Introductionmentioning

confidence: 99%

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Purification and Classification of Three Lipovitellin Subtypes in the Marbled Sole (Pleuronectes yokohamae)

et al. 2009

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An immunologlcal analysis using subtype-specific antisera of the major yolk protein lipovltellin (Lv) of the grey mullet (Mugil cephalus) confirmed the presence of the three corresponding Lv subtypes (LvA, LvB, and LvC) in vitellogenic ovaries of the marbled sole (Pleuronectes yokohamae). These three Lv subtypes were purified from sole ovaries by using various combinations of anion exchange, hydroxylapatite, immunoadsorbent, and gel-filtration chromatography. Purified LvA, LvB, and LvC had an apparent native mass of approximately 482, approximately 380, and approximately 372 kDa, respectively, estimated by gel filtration. Analysis of their tertiary structures by SDS-PAGE indicated that LvA, LvB, and LvC were typical of teleost Lvs in having a heavy (H) chain (approximately 105, approximately 102, and approximately 107 kDa, respectively) and a light (L) chain (approximately 22, approximately 19.5, and approximately 25 kDa, respectively). The N-termlnal amino acid (AA) sequences were obtained for the LvA H chain, the LvB H and L chains, and the LvC L chain and compared to the deduced AA sequences of their precursors, vitellogenins (Vgs), in several species. This comparison of LvA, LvB, and LvC with various teleost VgA, VgB, and VgC sequences, respectively, revealed high identities (60-100%). The purified Lv subtypes were subjected to double immunodiffusion using an antiserum against an unclassified Lv of the sole ( Hashimoto et al., 1998 ); only the LvB subtype exhibited immunoreactivity with this antiserum. This result indicates that the previously developed immunoassay using this anti-Lv for the detection of sole Vg is effectively a VgB-specific assay.

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Colorimetric Enantiorecognition of Oligopeptide and Logic Gate Construction Based on DNA Aptamer–Ligand–Gold Nanoparticle Interactions

Ren¹,

Wang²,

Wang³

et al. 2012

Chemistry A European J

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At the gate: Colorimetric enantiorecognition of one oligopeptide was realized by virtue of a D‐enantiomer specific DNA aptamer as chiral selector and unmodified gold nanoparticles as indicator. Oligopeptide enantiomers as inputs were introduced into colorimetric logic gates, which have the potential for enantiorecognition of oligopeptides (see figure). This system may lead to a better understanding of chiral recognition for information processing in natural systems.

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Development of a Sensitive Luminometric Immunoassay for Determining Baseline Seasonal Changes in Serum Vitellogenin Levels in Male Flounder (Pleuronectes Yokohamae)

Cited by 9 publications

References 17 publications

Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

Purification and Classification of Three Lipovitellin Subtypes in the Marbled Sole (Pleuronectes yokohamae)

Colorimetric Enantiorecognition of Oligopeptide and Logic Gate Construction Based on DNA Aptamer–Ligand–Gold Nanoparticle Interactions

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