Development of a sensitive polymerase chain reaction (PCR) and digoxigenin (DIG)-labeled in situ hybridization (ISH) for the detection of Vibrio bacteria in the Pacific oyster Crassostrea gigas

Kett, Gary F.; Culloty, Sarah C.; Jansen, Marcel A. K.; Lynch, Sharon A.

doi:10.1016/j.aqrep.2021.100961

Cited by 2 publications

(1 citation statement)

References 51 publications

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“…Moreover, some pathogenic bacteria from this genus may infect humans and aquaculture [85]. Another possible explanation for the enrichment of Vibrio was the high-density mariculture of coastal waters [86].…”

Section: Bacterial Relationships Within the Sediment Microbial Commun...mentioning

confidence: 99%

The Effect of the Artificial Reef on the Structure and Function of Sediment Bacterial Community

et al. 2022

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The bacterial community in sediment is sensitive to artificial disturbance, and they respond differently to human disturbance, such as changing the nutrient cycling and energy flow in marine ecosystems. However, little is known about the dynamics and distribution of bacterial community structures in marine sediments and potential biogeochemical functions during the long-time succession in marine ranching. In the present study, we compared the dynamics of the bacterial composition and potential biogeochemical functions of sediment to ten years (TR) and one-year new artificial reef (NR) areas using metagenomic next-generation sequencing technology. Results revealed that NR reduces the Pielou’s evenness and Shannon index. Similarly, nonmetric multidimensional scaling showed that the beta diversity of sediment bacterial communities in NR significantly differed between TR and non-artificial reef areas. Previously, TR biomarkers were frequently associated with organic matter decomposing and assimilating in the organically enriched sediments (i.e., Acinetobacter). The soluble reactive phosphate (SRP) and total phosphorus (TP) concentrations were thought to be the primary driving forces in shaping the microbial community in sediment. Pseudomonas, Lactobacillus, and Ralstonia have a significant positive correlation with SRP, TP, nitrate, and TN, but a negative association with pH, Salinity, Hg, and depth. NR was found to have more negative correlation nodes, indicating that taxa face more competition or predation press. Vibrio served as the module-hubs in the network in all areas. In addition, chemoheterotrophy, aerobic chemoheterotrophy, and fermentation were the three most prominent functions of the three areas, accounting for 59.96% of the relative abundance of the functional annotation. Different bacteria in sediments may change the amount of biogeochemical cycle in the marine ranching ecosystem. These findings can increase our understanding of the succession of the microecosystem for the marine ranching sedimentary environment by revealing how artificial reefs affect the indigenous sediment bacterial community and their responses to environmental variation.

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Section: Bacterial Relationships Within the Sediment Microbial Commun...mentioning

confidence: 99%

The Effect of the Artificial Reef on the Structure and Function of Sediment Bacterial Community

et al. 2022

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Immunomodulatory and Antiviral Effects of Macroalgae Sulphated Polysaccharides: Case Studies Extend Knowledge on Their Importance in Enhancing Shellfish Health, and the Control of a Global Viral Pathogen Ostreid Herpesvirus-1 microVar

et al. 2021

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Macroalgae are the primary source of non-animal sulphated polysaccharides (SPs) in the marine environment with fucoidans derived from brown algae (Phaeophyta) and carrageenans from red algae (Rhodophyta). Much research has been carried out on SP effects on Asian shrimp species (genera Penaeus and Metapenaeus) but their effect on commercially important bivalve mollusc species is limited and in Pacific oyster Crassostrea gigas is unknown. Knowledge of their impact on bivalve pathogens and Palaemon shrimp is unknown. The objectives of this study were to assess the effects of Fucus vesiculosus (Phaeophyta), Mastocarpus stellatus (Rhodophyta) and algal derivatives (fucoidan and κ-carrageenan) on C. gigas performance, and on ostreid herpesvirus-1 microvar (OsHV-1 μVar) and bacteria Vibrio spp. development. Both pathogens have been associated with significant oyster mortalities and economic losses globally. The effects of sulphated galactan from Gracilaria fisheri (Rhodophyta) on European common prawn Palaemon serratus, an important fishery species, was also assessed. Findings indicate a rapid and prolonged increase in total blood cell count, lysozyme (enzyme that destroys pathogens), and a difference in the ratio of blood cell types in treated individuals compared to their control counterparts. A significantly lower OsHV-1 μVar prevalence was observed in treated oysters and κ-carrageenan was found to suppress viral replication (loads), while OsHV-1 μVar was not detected in the fucoidan treated oysters from Day 8 of the 26-day trial. No antibacterial effect was observed however, the oysters did not succumb to vibriosis. These findings contribute further knowledge to macroalgae sulphated polysaccharide biotherapeutic properties, their twofold effect on animal health and viral suppression.

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Development of a sensitive polymerase chain reaction (PCR) and digoxigenin (DIG)-labeled in situ hybridization (ISH) for the detection of Vibrio bacteria in the Pacific oyster Crassostrea gigas

Cited by 2 publications

References 51 publications

The Effect of the Artificial Reef on the Structure and Function of Sediment Bacterial Community

The Effect of the Artificial Reef on the Structure and Function of Sediment Bacterial Community

Immunomodulatory and Antiviral Effects of Macroalgae Sulphated Polysaccharides: Case Studies Extend Knowledge on Their Importance in Enhancing Shellfish Health, and the Control of a Global Viral Pathogen Ostreid Herpesvirus-1 microVar

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